Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds
<b>Introduction:</b> <i>Streptococcus uberis</i> is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to underst...
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2025-03-01
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| author | Paola A. Rios Agudelo Julián Reyes Vélez Martha Olivera Angel Adam M. Blanchard Yesid Cuesta Astroz Arley Caraballo Guzmán Giovanny Torres Lindarte |
| author_facet | Paola A. Rios Agudelo Julián Reyes Vélez Martha Olivera Angel Adam M. Blanchard Yesid Cuesta Astroz Arley Caraballo Guzmán Giovanny Torres Lindarte |
| author_sort | Paola A. Rios Agudelo |
| collection | DOAJ |
| description | <b>Introduction:</b> <i>Streptococcus uberis</i> is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to understand its complex epidemiology and behavior against antimicrobials. <b>Methods:</b> A comparative genomic analysis of 10 <i>S. uberis</i> strains was performed and their antimicrobial susceptibility was assessed. <b>Results:</b> Ten different novel sequence types were found, and genes (<i>tetM, tetO</i>, <i>patB</i>, <i>lnuC</i>, <i>lnuA</i>, <i>lsaE</i>, <i>ermB</i>, <i>ANT</i>(6)-la) and mobile genetic elements previously associated with antimicrobial resistance (repUS43, IS<i>Sag</i>2, and IS<i>Enfa</i>4) and virulence (315.2 phage) were detected. Additionally, our strains had the highest relative rate of recombination to mutation (8.3) compared to other <i>S. uberis</i> strains isolated from different continents (America: 7.7, Asia: 2.9, Europe: 5.4, and Oceania: 6.6). Most of the strains (80%) tested showed phenotypic resistance to clindamycin and 70% exhibited intermediate susceptibility to penicillin. <b>Conclusions:</b> The high heterogeneity of strains observed and the presence of genetic factors linked to antimicrobial resistance represent a challenge for the implementation and surveillance of measures focused on the control and elimination of this pathogen. |
| format | Article |
| id | doaj-art-2736bb7949924d258bfbe4d6ca7e0a5d |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-2736bb7949924d258bfbe4d6ca7e0a5d2025-08-20T02:42:38ZengMDPI AGAntibiotics2079-63822025-03-0114329710.3390/antibiotics14030297Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy HerdsPaola A. Rios Agudelo0Julián Reyes Vélez1Martha Olivera Angel2Adam M. Blanchard3Yesid Cuesta Astroz4Arley Caraballo Guzmán5Giovanny Torres Lindarte6Instituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, ColombiaInstituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, ColombiaBiogenesis Research Group, Faculty of Agriculture Science, University of Antioquia, Cra 75 No. 65-87, Medellín 050010, Antioquia, ColombiaSchool of Veterinary and Science, University of Nottingham, Sutton Bonington Campus, Leicestireshire LE12 5RD, UKInstituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, ColombiaInstituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, ColombiaInstituto Colombiano de Medicina Tropical, Universidad CES, Cra 43a No. 52 Sur 99, Sabaneta 055450, Antioquia, Colombia<b>Introduction:</b> <i>Streptococcus uberis</i> is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to understand its complex epidemiology and behavior against antimicrobials. <b>Methods:</b> A comparative genomic analysis of 10 <i>S. uberis</i> strains was performed and their antimicrobial susceptibility was assessed. <b>Results:</b> Ten different novel sequence types were found, and genes (<i>tetM, tetO</i>, <i>patB</i>, <i>lnuC</i>, <i>lnuA</i>, <i>lsaE</i>, <i>ermB</i>, <i>ANT</i>(6)-la) and mobile genetic elements previously associated with antimicrobial resistance (repUS43, IS<i>Sag</i>2, and IS<i>Enfa</i>4) and virulence (315.2 phage) were detected. Additionally, our strains had the highest relative rate of recombination to mutation (8.3) compared to other <i>S. uberis</i> strains isolated from different continents (America: 7.7, Asia: 2.9, Europe: 5.4, and Oceania: 6.6). Most of the strains (80%) tested showed phenotypic resistance to clindamycin and 70% exhibited intermediate susceptibility to penicillin. <b>Conclusions:</b> The high heterogeneity of strains observed and the presence of genetic factors linked to antimicrobial resistance represent a challenge for the implementation and surveillance of measures focused on the control and elimination of this pathogen.https://www.mdpi.com/2079-6382/14/3/297bovine mastitis<i>Streptococcus uberis</i>whole genome sequencingrecombination eventsmobile genetic elements |
| spellingShingle | Paola A. Rios Agudelo Julián Reyes Vélez Martha Olivera Angel Adam M. Blanchard Yesid Cuesta Astroz Arley Caraballo Guzmán Giovanny Torres Lindarte Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds Antibiotics bovine mastitis <i>Streptococcus uberis</i> whole genome sequencing recombination events mobile genetic elements |
| title | Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds |
| title_full | Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds |
| title_fullStr | Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds |
| title_full_unstemmed | Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds |
| title_short | Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds |
| title_sort | whole genome sequencing analysis revealed high genomic variability recombination events and mobile genetic elements in i streptococcus uberis i strains isolated from bovine mastitis in colombian dairy herds |
| topic | bovine mastitis <i>Streptococcus uberis</i> whole genome sequencing recombination events mobile genetic elements |
| url | https://www.mdpi.com/2079-6382/14/3/297 |
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