Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds

Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements in <i>Streptococcus uberis</i> Strains Isolated from Bovine Mastitis in Colombian Dairy Herds

<b>Introduction:</b> <i>Streptococcus uberis</i> is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to underst...

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Main Authors: Paola A. Rios Agudelo, Julián Reyes Vélez, Martha Olivera Angel, Adam M. Blanchard, Yesid Cuesta Astroz, Arley Caraballo Guzmán, Giovanny Torres Lindarte
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Antibiotics
Subjects:
bovine mastitis
<i>Streptococcus uberis</i>
whole genome sequencing
recombination events
mobile genetic elements
Online Access:https://www.mdpi.com/2079-6382/14/3/297
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Summary:<b>Introduction:</b> <i>Streptococcus uberis</i> is a poorly controlled cause of bovine intramammary infections and a common motivation for the use antibiotics in dairy farms worldwide. Therefore, studying the genomic characteristics of this pathogen is fundamental to understand its complex epidemiology and behavior against antimicrobials. <b>Methods:</b> A comparative genomic analysis of 10 <i>S. uberis</i> strains was performed and their antimicrobial susceptibility was assessed. <b>Results:</b> Ten different novel sequence types were found, and genes (<i>tetM, tetO</i>, <i>patB</i>, <i>lnuC</i>, <i>lnuA</i>, <i>lsaE</i>, <i>ermB</i>, <i>ANT</i>(6)-la) and mobile genetic elements previously associated with antimicrobial resistance (repUS43, IS<i>Sag</i>2, and IS<i>Enfa</i>4) and virulence (315.2 phage) were detected. Additionally, our strains had the highest relative rate of recombination to mutation (8.3) compared to other <i>S. uberis</i> strains isolated from different continents (America: 7.7, Asia: 2.9, Europe: 5.4, and Oceania: 6.6). Most of the strains (80%) tested showed phenotypic resistance to clindamycin and 70% exhibited intermediate susceptibility to penicillin. <b>Conclusions:</b> The high heterogeneity of strains observed and the presence of genetic factors linked to antimicrobial resistance represent a challenge for the implementation and surveillance of measures focused on the control and elimination of this pathogen.
ISSN:2079-6382

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