GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing

<b>Background/Objectives:</b> Over the last decade, the development of innovative wound products has continued to be a focus of intense research to meet the huge demand of patients. The aim of this work was to develop novel medicated Spanish broom wound dressings capable of releasing <...

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Main Authors: Martina Rossi, Simone Rossello, Valentina Sallustio, Manuela Mandrone, Teresa Cerchiara, Ilaria Chiocchio, Giuseppe Chidichimo, Michele Protti, Laura Mercolini, Barbara Luppi, Federica Bigucci, Angela Abruzzo, Concettina Cappadone
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Pharmaceutics
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Online Access:https://www.mdpi.com/1999-4923/17/5/632
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author Martina Rossi
Simone Rossello
Valentina Sallustio
Manuela Mandrone
Teresa Cerchiara
Ilaria Chiocchio
Giuseppe Chidichimo
Michele Protti
Laura Mercolini
Barbara Luppi
Federica Bigucci
Angela Abruzzo
Concettina Cappadone
author_facet Martina Rossi
Simone Rossello
Valentina Sallustio
Manuela Mandrone
Teresa Cerchiara
Ilaria Chiocchio
Giuseppe Chidichimo
Michele Protti
Laura Mercolini
Barbara Luppi
Federica Bigucci
Angela Abruzzo
Concettina Cappadone
author_sort Martina Rossi
collection DOAJ
description <b>Background/Objectives:</b> Over the last decade, the development of innovative wound products has continued to be a focus of intense research to meet the huge demand of patients. The aim of this work was to develop novel medicated Spanish broom wound dressings capable of releasing <i>Rosa canina</i> extract, recognized for its high antioxidant activity. <b>Methods:</b><i>Rosa canina</i> extract was encapsulated in two different nanocarriers, namely glycethosomes and allanthosomes. The physico-chemical and functional characteristics of the obtained vesicles were described, including their size, particle size distribution, ζ potential, and encapsulation efficiency (EE). In addition, vesicles cytotoxicity and cell proliferation were evaluated on human fibroblasts. Furthermore, loaded vesicles were sunk into Spanish broom dressings, analyzed by confocal microscopy, and, finally, evaluated for their wound healing ability by scratch test. <b>Results:</b> Both carriers are nanometric in size, with a good EE (>70%), and a negative ζ potential. Additionally, vesicles are biosafe, non-cytotoxic, and lead to complete closure of the scratch in about 30 h. <b>Conclusions:</b> The findings showed that the developed Spanish broom dressings have the potential to be an efficient and innovative wound care product for accelerating skin wounds.
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spelling doaj-art-272e3de21c76406a89c276a4daecb8cb2025-08-20T03:47:54ZengMDPI AGPharmaceutics1999-49232025-05-0117563210.3390/pharmaceutics17050632GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound HealingMartina Rossi0Simone Rossello1Valentina Sallustio2Manuela Mandrone3Teresa Cerchiara4Ilaria Chiocchio5Giuseppe Chidichimo6Michele Protti7Laura Mercolini8Barbara Luppi9Federica Bigucci10Angela Abruzzo11Concettina Cappadone12Drug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyPharmaceutical Botany Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyPharmaceutical Botany Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Irnerio 42, 40126 Bologna, ItalyDepartment of Chemistry and Chemical Technologies, Università della Calabria, Via P. Bucci, 87036 Arcavacata di Rende, ItalyPharmaco-Toxicological Analysis (PTA Laboratory), Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, 40126 Bologna, ItalyPharmaco-Toxicological Analysis (PTA Laboratory), Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via Belmeloro 6, 40126 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyDrug Delivery Research Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, ItalyPharmaceutical Biochemistry Laboratory, Department of Pharmacy and Biotechnology, Alma Mater Studiorum, University of Bologna, Via San Donato 19/2, 40127 Bologna, Italy<b>Background/Objectives:</b> Over the last decade, the development of innovative wound products has continued to be a focus of intense research to meet the huge demand of patients. The aim of this work was to develop novel medicated Spanish broom wound dressings capable of releasing <i>Rosa canina</i> extract, recognized for its high antioxidant activity. <b>Methods:</b><i>Rosa canina</i> extract was encapsulated in two different nanocarriers, namely glycethosomes and allanthosomes. The physico-chemical and functional characteristics of the obtained vesicles were described, including their size, particle size distribution, ζ potential, and encapsulation efficiency (EE). In addition, vesicles cytotoxicity and cell proliferation were evaluated on human fibroblasts. Furthermore, loaded vesicles were sunk into Spanish broom dressings, analyzed by confocal microscopy, and, finally, evaluated for their wound healing ability by scratch test. <b>Results:</b> Both carriers are nanometric in size, with a good EE (>70%), and a negative ζ potential. Additionally, vesicles are biosafe, non-cytotoxic, and lead to complete closure of the scratch in about 30 h. <b>Conclusions:</b> The findings showed that the developed Spanish broom dressings have the potential to be an efficient and innovative wound care product for accelerating skin wounds.https://www.mdpi.com/1999-4923/17/5/632<i>Rosa canina</i> L. extractSpanish broomwound dressingsencapsulationglycethosomesallanthosomes
spellingShingle Martina Rossi
Simone Rossello
Valentina Sallustio
Manuela Mandrone
Teresa Cerchiara
Ilaria Chiocchio
Giuseppe Chidichimo
Michele Protti
Laura Mercolini
Barbara Luppi
Federica Bigucci
Angela Abruzzo
Concettina Cappadone
GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
Pharmaceutics
<i>Rosa canina</i> L. extract
Spanish broom
wound dressings
encapsulation
glycethosomes
allanthosomes
title GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
title_full GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
title_fullStr GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
title_full_unstemmed GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
title_short GinExtraMed: Focus on <i>Rosa canina</i> L. Extract Encapsulated into Glycethosomes and Allanthosomes for Accelerating Skin Wound Healing
title_sort ginextramed focus on i rosa canina i l extract encapsulated into glycethosomes and allanthosomes for accelerating skin wound healing
topic <i>Rosa canina</i> L. extract
Spanish broom
wound dressings
encapsulation
glycethosomes
allanthosomes
url https://www.mdpi.com/1999-4923/17/5/632
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