Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan
Graphical Abstract Highlight Research • Bioflocculation technique can improve the harvesting effectiveness of semimass culture of Euglena sp. • The addition of E. texensis can significantly increase the flocculation efficiency of Euglena sp. • The addition of commercial chitosan was ab...
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| Language: | English |
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Faculty of Fisheries and Marine Universitas Airlangga
2025-01-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/57417 |
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| author | Eko Agus Suyono Siti Mudrikah Khalid Erlangga Karilanata Dedy Kurnianto Arief Budiman Nugroho Dewayanto Renata Adaranyssa Egistha Putri Brilian Ryan Sadewo Tia Erfianti |
| author_facet | Eko Agus Suyono Siti Mudrikah Khalid Erlangga Karilanata Dedy Kurnianto Arief Budiman Nugroho Dewayanto Renata Adaranyssa Egistha Putri Brilian Ryan Sadewo Tia Erfianti |
| author_sort | Eko Agus Suyono |
| collection | DOAJ |
| description | Graphical Abstract
Highlight Research
• Bioflocculation technique can improve the harvesting effectiveness of semimass culture of Euglena sp.
• The addition of E. texensis can significantly increase the flocculation efficiency of Euglena sp.
• The addition of commercial chitosan was able to increase the flocculation efficiency of Euglena sp.
• The biomass and lipid content produced by Euglena sp. with E. texensis flocculant agent showed higher results than the biomass and lipid content produced by Euglena sp. with commercial chitosan flocculant agent.
Abstract
Euglena sp. has a high potential to be developed as biofuel. However, the high cost and energy required for the harvesting process are hindering the production. Flocculation using natural substances, such as microorganisms and biopolymers, offers a promising solution to minimize energy and production costs, so it is applicable on a mass scale. Ettlia texensis is one of the autoflocculating microalgae that can excrete extracellular polymeric substances (EPS). Chitosan is a linear copolymer of D-glucosamine and N-acetyl-D-glucosamine produced by the deacetylation of chitin, which is usually exploited by marine crustaceans, shrimp, and crabs. Chitosan has a very high cation load, so it is often used for coagulation or flocculation. This study explores the potential of E. texensis and chitosan as flocculant agents to harvest the mass culture of Euglena sp. by giving different doses E. texensis with 1:0.25 (E3), 1:0.5 (E4), 1:1 (E5), and 1:2 (E6), and chitosan with 1.25 mg (C1), 2.5 mg (C2), 3.75 mg (C3), and 5 mg (C4). This research began with the cultivation of Euglena sp. and E. texensis on a 50 L scale for 12 days. The effectiveness of flocculation was measured by the spectrophotometric method. Based on this research, the best treatment for harvesting Euglena sp. culture by bioflocculation was shown by the addition of chitosan (5 mg) with the recovery of 84.83%, 0.2213 mg/mL biomass, and 0.2117 mg/mL lipid content. Meanwhile, with E. texensis, the best was shown by the ratio of 1:2 with recovery 84.71%, 0.2053 mg/mL biomass, and 0.1753 mg/mL |
| format | Article |
| id | doaj-art-2726d70441ac4e0bb870237858df0f7d |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-2726d70441ac4e0bb870237858df0f7d2025-08-20T03:36:26ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592025-01-0117226027510.20473/jipk.v17i2.5741755582Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial ChitosanEko Agus Suyono0https://orcid.org/0000-0002-9208-4541Siti Mudrikah1https://orcid.org/0009-0009-1203-5060Khalid Erlangga Karilanata2https://orcid.org/0009-0000-2374-2267Dedy Kurnianto3https://orcid.org/0000-0002-4912-6400Arief Budiman4https://orcid.org/0000-0003-4846-8270Nugroho Dewayanto5https://orcid.org/0000-0002-9435-5675Renata Adaranyssa Egistha Putri6https://orcid.org/0000-0003-0541-685XBrilian Ryan Sadewo7https://orcid.org/0000-0002-7074-9055Tia Erfianti8https://orcid.org/0000-0002-5462-1131Faculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaResearch Centre for Food Technology and Processing, National Research and Innovation Agency, Gunungkidul, YogyakartaMaster Program in System Engineering, Universitas Gadjah Mada, Yogyakarta, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Yogyakarta, IndonesiaStudy Program of Biotechnology, Graduate School of Universitas Gadjah Mada,Yogyakarta, Indonesia Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaFaculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. IndonesiaGraphical Abstract Highlight Research • Bioflocculation technique can improve the harvesting effectiveness of semimass culture of Euglena sp. • The addition of E. texensis can significantly increase the flocculation efficiency of Euglena sp. • The addition of commercial chitosan was able to increase the flocculation efficiency of Euglena sp. • The biomass and lipid content produced by Euglena sp. with E. texensis flocculant agent showed higher results than the biomass and lipid content produced by Euglena sp. with commercial chitosan flocculant agent. Abstract Euglena sp. has a high potential to be developed as biofuel. However, the high cost and energy required for the harvesting process are hindering the production. Flocculation using natural substances, such as microorganisms and biopolymers, offers a promising solution to minimize energy and production costs, so it is applicable on a mass scale. Ettlia texensis is one of the autoflocculating microalgae that can excrete extracellular polymeric substances (EPS). Chitosan is a linear copolymer of D-glucosamine and N-acetyl-D-glucosamine produced by the deacetylation of chitin, which is usually exploited by marine crustaceans, shrimp, and crabs. Chitosan has a very high cation load, so it is often used for coagulation or flocculation. This study explores the potential of E. texensis and chitosan as flocculant agents to harvest the mass culture of Euglena sp. by giving different doses E. texensis with 1:0.25 (E3), 1:0.5 (E4), 1:1 (E5), and 1:2 (E6), and chitosan with 1.25 mg (C1), 2.5 mg (C2), 3.75 mg (C3), and 5 mg (C4). This research began with the cultivation of Euglena sp. and E. texensis on a 50 L scale for 12 days. The effectiveness of flocculation was measured by the spectrophotometric method. Based on this research, the best treatment for harvesting Euglena sp. culture by bioflocculation was shown by the addition of chitosan (5 mg) with the recovery of 84.83%, 0.2213 mg/mL biomass, and 0.2117 mg/mL lipid content. Meanwhile, with E. texensis, the best was shown by the ratio of 1:2 with recovery 84.71%, 0.2053 mg/mL biomass, and 0.1753 mg/mLhttps://e-journal.unair.ac.id/JIPK/article/view/57417 |
| spellingShingle | Eko Agus Suyono Siti Mudrikah Khalid Erlangga Karilanata Dedy Kurnianto Arief Budiman Nugroho Dewayanto Renata Adaranyssa Egistha Putri Brilian Ryan Sadewo Tia Erfianti Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan Jurnal Ilmiah Perikanan dan Kelautan |
| title | Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan |
| title_full | Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan |
| title_fullStr | Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan |
| title_full_unstemmed | Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan |
| title_short | Harvesting Optimization, Biomass, and Lipid Content Analysis of Euglena sp. Culture with Ettlia texensis Bioflocculant and Commercial Chitosan |
| title_sort | harvesting optimization biomass and lipid content analysis of euglena sp culture with ettlia texensis bioflocculant and commercial chitosan |
| url | https://e-journal.unair.ac.id/JIPK/article/view/57417 |
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