Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)

This study aimed to develop for in vitro germination of zygotic embryos and to establish an acclimatization procedure for the production of Butia yatay palm plants under controlled conditions. B. yatay fruits were mechanically scarified for seeds extraction prior to isolation of zygotic embryos. Emb...

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Main Authors: Flachsland Eduardo A., Terada Graciela, Mignolli Francesco, Vidoz María L., Medina Ricardo D.
Format: Article
Language:English
Published: Sciendo 2025-06-01
Series:Journal of Horticultural Research
Subjects:
Online Access:https://doi.org/10.2478/johr-2025-0008
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author Flachsland Eduardo A.
Terada Graciela
Mignolli Francesco
Vidoz María L.
Medina Ricardo D.
author_facet Flachsland Eduardo A.
Terada Graciela
Mignolli Francesco
Vidoz María L.
Medina Ricardo D.
author_sort Flachsland Eduardo A.
collection DOAJ
description This study aimed to develop for in vitro germination of zygotic embryos and to establish an acclimatization procedure for the production of Butia yatay palm plants under controlled conditions. B. yatay fruits were mechanically scarified for seeds extraction prior to isolation of zygotic embryos. Embryos were cultured on Murashige and Skoog medium with a combination of plant growth regulators. Acclimatization consisted of transferring plants to sterilized or unsterilized soil-sand substrates, with or without polyethylene cover. Embryos cultivated on Murashige and Skoog medium with 0.1 mg·L−1 1-naphthaleneacetic acid and 0.1 mg·L−1 6-benzylaminopurine achieved 90% germination. After fungicide treatment and transplanting into sterilized soil mix in shaded conditions, the plants were covered with polyethylene, which resulted in a 67% acclimatization success rate.
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series Journal of Horticultural Research
spelling doaj-art-271df10ace52449ea932cb1d890c19c22025-08-20T02:44:34ZengSciendoJournal of Horticultural Research2353-39782025-06-01331495410.2478/johr-2025-0008Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)Flachsland Eduardo A.0Terada Graciela1Mignolli Francesco2Vidoz María L.3Medina Ricardo D.4Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2131, 3400 Corrientes, ArgentinaFacultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2131, 3400 Corrientes, ArgentinaFacultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2131, 3400 Corrientes, ArgentinaFacultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2131, 3400 Corrientes, ArgentinaFacultad de Ciencias Agrarias, Universidad Nacional del Nordeste (UNNE), Sargento Cabral 2131, 3400 Corrientes, ArgentinaThis study aimed to develop for in vitro germination of zygotic embryos and to establish an acclimatization procedure for the production of Butia yatay palm plants under controlled conditions. B. yatay fruits were mechanically scarified for seeds extraction prior to isolation of zygotic embryos. Embryos were cultured on Murashige and Skoog medium with a combination of plant growth regulators. Acclimatization consisted of transferring plants to sterilized or unsterilized soil-sand substrates, with or without polyethylene cover. Embryos cultivated on Murashige and Skoog medium with 0.1 mg·L−1 1-naphthaleneacetic acid and 0.1 mg·L−1 6-benzylaminopurine achieved 90% germination. After fungicide treatment and transplanting into sterilized soil mix in shaded conditions, the plants were covered with polyethylene, which resulted in a 67% acclimatization success rate.https://doi.org/10.2478/johr-2025-0008acclimatizationpalm treesterilization
spellingShingle Flachsland Eduardo A.
Terada Graciela
Mignolli Francesco
Vidoz María L.
Medina Ricardo D.
Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
Journal of Horticultural Research
acclimatization
palm tree
sterilization
title Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
title_full Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
title_fullStr Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
title_full_unstemmed Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
title_short Plant Production from in vitro Culture of Isolated Zygotic Embryos of Butia yatay (Mart.) Becc. (Arecaceae)
title_sort plant production from in vitro culture of isolated zygotic embryos of butia yatay mart becc arecaceae
topic acclimatization
palm tree
sterilization
url https://doi.org/10.2478/johr-2025-0008
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