Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing

Seroma formation and poor wound healing are common complications of many surgeries that create anatomical dead space (i.e., mastectomy), often causing tissue infection and even necrosis. Although negative pressure drainage and tissue adhesives are investigated to alleviate fluid accumulation post-su...

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Main Authors: Xinping Wang, Guoqing Wang, Jianfei Wang, Junqiang Xue, Gaoli Liu, Changjiang Fan
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590006424003740
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author Xinping Wang
Guoqing Wang
Jianfei Wang
Junqiang Xue
Gaoli Liu
Changjiang Fan
author_facet Xinping Wang
Guoqing Wang
Jianfei Wang
Junqiang Xue
Gaoli Liu
Changjiang Fan
author_sort Xinping Wang
collection DOAJ
description Seroma formation and poor wound healing are common complications of many surgeries that create anatomical dead space (i.e., mastectomy), often causing tissue infection and even necrosis. Although negative pressure drainage and tissue adhesives are investigated to alleviate fluid accumulation post-surgery, however, their therapeutic efficacy remains unsatisfactory in most cases. Herein, the catechol-rich chemically crosslinked gelatin microspheres (ca-CGMSs) have been developed as biodegradable reconstructive implants for preventing seroma formation and concurrently promoting subcutaneous wound healing. Compared with the most representative hydrogel adhesive, i.e. commercial porcine fibrin sealant (PFS), the loosely packed ca-CGMSs with diameters range from 50 to 350 μm, provide numerous cell-adhesive interfaces and interconnected macro-pores for enhanced cell adhesion, proliferation and migration. Subcutaneous embedding trials show the in situ swelling aggregation and wet tissue adhesion of ca-CGMSs as well as their capacity in recruiting autologous cells in rat mastectomy models. The trials in rabbit mastectomy models demonstrate that, compared with PFS gluing, the implanted dried ca-CGMSs not only significantly inhibit seroma formation, but also achieve enhanced wound healing by inducing the formation of vascularized neo-tissue. The ca-CGMSs show a great potential to be the next-generation of restorative materials for both preventing seroma formation and healing subcutaneous wounds.
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spelling doaj-art-2718e6007b3d4fca960997ce9fa7fb8e2025-08-20T01:55:30ZengElsevierMaterials Today Bio2590-00642024-12-012910131310.1016/j.mtbio.2024.101313Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healingXinping Wang0Guoqing Wang1Jianfei Wang2Junqiang Xue3Gaoli Liu4Changjiang Fan5Institute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao, 266021, Shandong, PR ChinaDepartment of Cardiovascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao Medical College, College of Medicine, Qingdao University, Qingdao, 266021, Shandong, PR ChinaInstitute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao, 266021, Shandong, PR ChinaDepartment of Rehabilitation Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao, 266000, Shandong, PR ChinaDepartment of Cardiovascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao Medical College, College of Medicine, Qingdao University, Qingdao, 266021, Shandong, PR ChinaInstitute for Translational Medicine, The Affiliated Hospital of Qingdao University, Qingdao Medical College, Qingdao University, Qingdao, 266021, Shandong, PR China; Corresponding author. Institute for Translational Medicine The Affiliated Hospital of Qingdao University Qingdao University Qingdao, 266021, PR China.Seroma formation and poor wound healing are common complications of many surgeries that create anatomical dead space (i.e., mastectomy), often causing tissue infection and even necrosis. Although negative pressure drainage and tissue adhesives are investigated to alleviate fluid accumulation post-surgery, however, their therapeutic efficacy remains unsatisfactory in most cases. Herein, the catechol-rich chemically crosslinked gelatin microspheres (ca-CGMSs) have been developed as biodegradable reconstructive implants for preventing seroma formation and concurrently promoting subcutaneous wound healing. Compared with the most representative hydrogel adhesive, i.e. commercial porcine fibrin sealant (PFS), the loosely packed ca-CGMSs with diameters range from 50 to 350 μm, provide numerous cell-adhesive interfaces and interconnected macro-pores for enhanced cell adhesion, proliferation and migration. Subcutaneous embedding trials show the in situ swelling aggregation and wet tissue adhesion of ca-CGMSs as well as their capacity in recruiting autologous cells in rat mastectomy models. The trials in rabbit mastectomy models demonstrate that, compared with PFS gluing, the implanted dried ca-CGMSs not only significantly inhibit seroma formation, but also achieve enhanced wound healing by inducing the formation of vascularized neo-tissue. The ca-CGMSs show a great potential to be the next-generation of restorative materials for both preventing seroma formation and healing subcutaneous wounds.http://www.sciencedirect.com/science/article/pii/S2590006424003740Wound healingMicrosphereSeromaCatecholTissue engineeringGelatin
spellingShingle Xinping Wang
Guoqing Wang
Jianfei Wang
Junqiang Xue
Gaoli Liu
Changjiang Fan
Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
Materials Today Bio
Wound healing
Microsphere
Seroma
Catechol
Tissue engineering
Gelatin
title Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
title_full Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
title_fullStr Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
title_full_unstemmed Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
title_short Catechol-rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
title_sort catechol rich gelatin microspheres as restorative medical implants intended for inhibiting seroma formation and promoting wound healing
topic Wound healing
Microsphere
Seroma
Catechol
Tissue engineering
Gelatin
url http://www.sciencedirect.com/science/article/pii/S2590006424003740
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