Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates
A simple and sensitive high-performance liquid chromatography (HPLC) method with electrochemical detection (ECD) was developed for measuring N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ) in environmental samples. The system used C30 columns for sample separation and a dual-mode...
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Elsevier
2025-05-01
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| Series: | Journal of Chromatography Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391725000301 |
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| author | Natsumi Okawa Akira Ito Vonika Ka-Man Au Nozomu Tsuchiya Takayuki Kameda |
| author_facet | Natsumi Okawa Akira Ito Vonika Ka-Man Au Nozomu Tsuchiya Takayuki Kameda |
| author_sort | Natsumi Okawa |
| collection | DOAJ |
| description | A simple and sensitive high-performance liquid chromatography (HPLC) method with electrochemical detection (ECD) was developed for measuring N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ) in environmental samples. The system used C30 columns for sample separation and a dual-mode electrochemical detector with a reduction cell, followed by an oxidation/detection cell connected in series. Under the optimal reduction voltage (–600 mV vs. Au) and optimal oxidation voltage (+600 mV vs. Ag/AgCl), the assay demonstrated high accuracy (96 %–107 %) when applied to airborne particulate sample extracts spiked with known amounts of 6-PPDQ. The detection limit was 3 fmol per injection (signal-to-noise ratio = 3), with a calibration range of 0.1–100 pmol, exhibiting excellent linearity (R2 > 0.9995). Using this newly developed HPLC-ECD analytical method with simple sample preparation, daily variations in 6-PPDQ concentrations in airborne particles collected in Kyoto, Japan, ranging from 21 to 165 fmol m–3 (n = 20), were successfully measured, highlighting the high potential of this HPLC-ECD method for routine trace analysis in environmental samples. |
| format | Article |
| id | doaj-art-2718487c72584ff3b0accbb7af3beb4e |
| institution | OA Journals |
| issn | 2772-3917 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-2718487c72584ff3b0accbb7af3beb4e2025-08-20T02:05:20ZengElsevierJournal of Chromatography Open2772-39172025-05-01710023210.1016/j.jcoa.2025.100232Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulatesNatsumi Okawa0Akira Ito1Vonika Ka-Man Au2Nozomu Tsuchiya3Takayuki Kameda4Graduate School of Energy Science, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto, 606-8501, JapanEicom Corporation, 113 Shimotobakita Enmenda-Cho, Fushimi-ku, Kyoto, 612–8497, JapanGraduate School of Energy Science, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto, 606-8501, JapanGraduate School of Energy Science, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto, 606-8501, JapanGraduate School of Energy Science, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto, 606-8501, Japan; Corresponding author.A simple and sensitive high-performance liquid chromatography (HPLC) method with electrochemical detection (ECD) was developed for measuring N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ) in environmental samples. The system used C30 columns for sample separation and a dual-mode electrochemical detector with a reduction cell, followed by an oxidation/detection cell connected in series. Under the optimal reduction voltage (–600 mV vs. Au) and optimal oxidation voltage (+600 mV vs. Ag/AgCl), the assay demonstrated high accuracy (96 %–107 %) when applied to airborne particulate sample extracts spiked with known amounts of 6-PPDQ. The detection limit was 3 fmol per injection (signal-to-noise ratio = 3), with a calibration range of 0.1–100 pmol, exhibiting excellent linearity (R2 > 0.9995). Using this newly developed HPLC-ECD analytical method with simple sample preparation, daily variations in 6-PPDQ concentrations in airborne particles collected in Kyoto, Japan, ranging from 21 to 165 fmol m–3 (n = 20), were successfully measured, highlighting the high potential of this HPLC-ECD method for routine trace analysis in environmental samples.http://www.sciencedirect.com/science/article/pii/S27723917250003016-PPD quinoneHPLC-ECDTire wear particlesAirborne particlesRoad dust |
| spellingShingle | Natsumi Okawa Akira Ito Vonika Ka-Man Au Nozomu Tsuchiya Takayuki Kameda Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates Journal of Chromatography Open 6-PPD quinone HPLC-ECD Tire wear particles Airborne particles Road dust |
| title | Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates |
| title_full | Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates |
| title_fullStr | Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates |
| title_full_unstemmed | Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates |
| title_short | Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates |
| title_sort | application of high performance liquid chromatography with electrochemical detection for the analysis of n 1 3 dimethylbutyl n phenyl p phenylenediamine quinone in ambient particulates |
| topic | 6-PPD quinone HPLC-ECD Tire wear particles Airborne particles Road dust |
| url | http://www.sciencedirect.com/science/article/pii/S2772391725000301 |
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