Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates

Application of high-performance liquid chromatography with electrochemical detection for the analysis of N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone in ambient particulates

A simple and sensitive high-performance liquid chromatography (HPLC) method with electrochemical detection (ECD) was developed for measuring N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ) in environmental samples. The system used C30 columns for sample separation and a dual-mode...

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Bibliographic Details
Main Authors: Natsumi Okawa, Akira Ito, Vonika Ka-Man Au, Nozomu Tsuchiya, Takayuki Kameda
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Journal of Chromatography Open
Subjects:
6-PPD quinone
HPLC-ECD
Tire wear particles
Airborne particles
Road dust
Online Access:http://www.sciencedirect.com/science/article/pii/S2772391725000301
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Summary:A simple and sensitive high-performance liquid chromatography (HPLC) method with electrochemical detection (ECD) was developed for measuring N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine quinone (6-PPDQ) in environmental samples. The system used C30 columns for sample separation and a dual-mode electrochemical detector with a reduction cell, followed by an oxidation/detection cell connected in series. Under the optimal reduction voltage (–600 mV vs. Au) and optimal oxidation voltage (+600 mV vs. Ag/AgCl), the assay demonstrated high accuracy (96 %–107 %) when applied to airborne particulate sample extracts spiked with known amounts of 6-PPDQ. The detection limit was 3 fmol per injection (signal-to-noise ratio = 3), with a calibration range of 0.1–100 pmol, exhibiting excellent linearity (R2 > 0.9995). Using this newly developed HPLC-ECD analytical method with simple sample preparation, daily variations in 6-PPDQ concentrations in airborne particles collected in Kyoto, Japan, ranging from 21 to 165 fmol m–3 (n = 20), were successfully measured, highlighting the high potential of this HPLC-ECD method for routine trace analysis in environmental samples.
ISSN:2772-3917

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