3D Culture of MSCs for Clinical Application
Mesenchymal stem cells (MSCs) play an important role in regenerative medicine and drug discovery due to their multipotential differentiation capabilities and immunomodulatory effects. Compared with traditional 2D cultures of MSCs, 3D cultures of MSCs have emerged as an effective approach to enhance...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/11/12/1199 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850059195285504000 |
|---|---|
| author | Qi Gao Mehmet Sertac Cekuc Yasemin Sude Ergul Alexa K. Pius Issei Shinohara Masatoshi Murayama Yosuke Susuki Chao Ma Mayu Morita Simon Kwoon-Ho Chow Stuart B. Goodman |
| author_facet | Qi Gao Mehmet Sertac Cekuc Yasemin Sude Ergul Alexa K. Pius Issei Shinohara Masatoshi Murayama Yosuke Susuki Chao Ma Mayu Morita Simon Kwoon-Ho Chow Stuart B. Goodman |
| author_sort | Qi Gao |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) play an important role in regenerative medicine and drug discovery due to their multipotential differentiation capabilities and immunomodulatory effects. Compared with traditional 2D cultures of MSCs, 3D cultures of MSCs have emerged as an effective approach to enhance cell viability, proliferation, and functionality, and provide a more relevant physiological environment. Here, we review the therapeutic potential of 3D-cultured MSCs, highlighting their roles in tissue regeneration and repair and drug screening. We further summarize successful cases that apply 3D MSCs in modeling disease states, enabling the identification of novel therapeutic strategies. Despite these promising applications, we discuss challenges that remain in the clinical translation of 3D MSC technologies, including stability, cell heterogeneity, and regulatory issues. We conclude by addressing these obstacles and emphasizing the need for further research to fully exploit the potential of 3D MSCs in clinical practice. |
| format | Article |
| id | doaj-art-271307d7bce2408c95b47868bb49fbc8 |
| institution | DOAJ |
| issn | 2306-5354 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-271307d7bce2408c95b47868bb49fbc82025-08-20T02:50:56ZengMDPI AGBioengineering2306-53542024-11-011112119910.3390/bioengineering111211993D Culture of MSCs for Clinical ApplicationQi Gao0Mehmet Sertac Cekuc1Yasemin Sude Ergul2Alexa K. Pius3Issei Shinohara4Masatoshi Murayama5Yosuke Susuki6Chao Ma7Mayu Morita8Simon Kwoon-Ho Chow9Stuart B. Goodman10Department of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USADepartment of Orthopaedic Surgery, Stanford University, Stanford, CA 94304, USAMesenchymal stem cells (MSCs) play an important role in regenerative medicine and drug discovery due to their multipotential differentiation capabilities and immunomodulatory effects. Compared with traditional 2D cultures of MSCs, 3D cultures of MSCs have emerged as an effective approach to enhance cell viability, proliferation, and functionality, and provide a more relevant physiological environment. Here, we review the therapeutic potential of 3D-cultured MSCs, highlighting their roles in tissue regeneration and repair and drug screening. We further summarize successful cases that apply 3D MSCs in modeling disease states, enabling the identification of novel therapeutic strategies. Despite these promising applications, we discuss challenges that remain in the clinical translation of 3D MSC technologies, including stability, cell heterogeneity, and regulatory issues. We conclude by addressing these obstacles and emphasizing the need for further research to fully exploit the potential of 3D MSCs in clinical practice.https://www.mdpi.com/2306-5354/11/12/1199MSCs3D culture technologyin vivo trials |
| spellingShingle | Qi Gao Mehmet Sertac Cekuc Yasemin Sude Ergul Alexa K. Pius Issei Shinohara Masatoshi Murayama Yosuke Susuki Chao Ma Mayu Morita Simon Kwoon-Ho Chow Stuart B. Goodman 3D Culture of MSCs for Clinical Application Bioengineering MSCs 3D culture technology in vivo trials |
| title | 3D Culture of MSCs for Clinical Application |
| title_full | 3D Culture of MSCs for Clinical Application |
| title_fullStr | 3D Culture of MSCs for Clinical Application |
| title_full_unstemmed | 3D Culture of MSCs for Clinical Application |
| title_short | 3D Culture of MSCs for Clinical Application |
| title_sort | 3d culture of mscs for clinical application |
| topic | MSCs 3D culture technology in vivo trials |
| url | https://www.mdpi.com/2306-5354/11/12/1199 |
| work_keys_str_mv | AT qigao 3dcultureofmscsforclinicalapplication AT mehmetsertaccekuc 3dcultureofmscsforclinicalapplication AT yaseminsudeergul 3dcultureofmscsforclinicalapplication AT alexakpius 3dcultureofmscsforclinicalapplication AT isseishinohara 3dcultureofmscsforclinicalapplication AT masatoshimurayama 3dcultureofmscsforclinicalapplication AT yosukesusuki 3dcultureofmscsforclinicalapplication AT chaoma 3dcultureofmscsforclinicalapplication AT mayumorita 3dcultureofmscsforclinicalapplication AT simonkwoonhochow 3dcultureofmscsforclinicalapplication AT stuartbgoodman 3dcultureofmscsforclinicalapplication |