Shot-Noise Limited Time-Encoded Raman Spectroscopy
Raman scattering, an inelastic scattering mechanism, provides information about molecular excitation energies and can be used to identify chemical compounds. Albeit being a powerful analysis tool, especially for label-free biomedical imaging with molecular contrast, it suffers from inherently low si...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2017/9253475 |
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| author | Sebastian Karpf Matthias Eibl Wolfgang Wieser Thomas Klein Robert Huber |
| author_facet | Sebastian Karpf Matthias Eibl Wolfgang Wieser Thomas Klein Robert Huber |
| author_sort | Sebastian Karpf |
| collection | DOAJ |
| description | Raman scattering, an inelastic scattering mechanism, provides information about molecular excitation energies and can be used to identify chemical compounds. Albeit being a powerful analysis tool, especially for label-free biomedical imaging with molecular contrast, it suffers from inherently low signal levels. This practical limitation can be overcome by nonlinear enhancement techniques like stimulated Raman scattering (SRS). In SRS, an additional light source stimulates the Raman scattering process. This can lead to orders of magnitude increase in signal levels and hence faster acquisition in biomedical imaging. However, achieving a broad spectral coverage in SRS is technically challenging and the signal is no longer background-free, as either stimulated Raman gain (SRG) or loss (SRL) is measured, turning a sensitivity limit into a dynamic range limit. Thus, the signal has to be isolated from the laser background light, requiring elaborate methods for minimizing detection noise. Here, we analyze the detection sensitivity of a shot-noise limited broadband stimulated time-encoded Raman (TICO-Raman) system in detail. In time-encoded Raman, a wavelength-swept Fourier domain mode locking (FDML) laser covers a broad range of Raman transition energies while allowing a dual-balanced detection for lowering the detection noise to the fundamental shot-noise limit. |
| format | Article |
| id | doaj-art-270931bb74184c6d9cd56cd420407c4c |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-270931bb74184c6d9cd56cd420407c4c2025-02-03T00:59:22ZengWileyJournal of Spectroscopy2314-49202314-49392017-01-01201710.1155/2017/92534759253475Shot-Noise Limited Time-Encoded Raman SpectroscopySebastian Karpf0Matthias Eibl1Wolfgang Wieser2Thomas Klein3Robert Huber4Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, CA, USAInstitut für Biomedizinische Optik, Universität zu Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, GermanyOptores GmbH, Gollierstr. 70, 80339 Munich, GermanyOptores GmbH, Gollierstr. 70, 80339 Munich, GermanyInstitut für Biomedizinische Optik, Universität zu Lübeck, Peter-Monnik-Weg 4, 23562 Lübeck, GermanyRaman scattering, an inelastic scattering mechanism, provides information about molecular excitation energies and can be used to identify chemical compounds. Albeit being a powerful analysis tool, especially for label-free biomedical imaging with molecular contrast, it suffers from inherently low signal levels. This practical limitation can be overcome by nonlinear enhancement techniques like stimulated Raman scattering (SRS). In SRS, an additional light source stimulates the Raman scattering process. This can lead to orders of magnitude increase in signal levels and hence faster acquisition in biomedical imaging. However, achieving a broad spectral coverage in SRS is technically challenging and the signal is no longer background-free, as either stimulated Raman gain (SRG) or loss (SRL) is measured, turning a sensitivity limit into a dynamic range limit. Thus, the signal has to be isolated from the laser background light, requiring elaborate methods for minimizing detection noise. Here, we analyze the detection sensitivity of a shot-noise limited broadband stimulated time-encoded Raman (TICO-Raman) system in detail. In time-encoded Raman, a wavelength-swept Fourier domain mode locking (FDML) laser covers a broad range of Raman transition energies while allowing a dual-balanced detection for lowering the detection noise to the fundamental shot-noise limit.http://dx.doi.org/10.1155/2017/9253475 |
| spellingShingle | Sebastian Karpf Matthias Eibl Wolfgang Wieser Thomas Klein Robert Huber Shot-Noise Limited Time-Encoded Raman Spectroscopy Journal of Spectroscopy |
| title | Shot-Noise Limited Time-Encoded Raman Spectroscopy |
| title_full | Shot-Noise Limited Time-Encoded Raman Spectroscopy |
| title_fullStr | Shot-Noise Limited Time-Encoded Raman Spectroscopy |
| title_full_unstemmed | Shot-Noise Limited Time-Encoded Raman Spectroscopy |
| title_short | Shot-Noise Limited Time-Encoded Raman Spectroscopy |
| title_sort | shot noise limited time encoded raman spectroscopy |
| url | http://dx.doi.org/10.1155/2017/9253475 |
| work_keys_str_mv | AT sebastiankarpf shotnoiselimitedtimeencodedramanspectroscopy AT matthiaseibl shotnoiselimitedtimeencodedramanspectroscopy AT wolfgangwieser shotnoiselimitedtimeencodedramanspectroscopy AT thomasklein shotnoiselimitedtimeencodedramanspectroscopy AT roberthuber shotnoiselimitedtimeencodedramanspectroscopy |