Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage
Aim. To investigate the effect of photodynamic action of LED-activated curcumin on cell viability, membrane permeability, and intracellular reactive oxygen species of Staphylococcus aureus. Methods. Staphylococcus aureus was incubated with the different concentrations of curcumin for 60 min and then...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/637601 |
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| _version_ | 1832551935271501824 |
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| author | Yuan Jiang Albert Wingnang Leung Heyu Hua Xiancai Rao Chuanshan Xu |
| author_facet | Yuan Jiang Albert Wingnang Leung Heyu Hua Xiancai Rao Chuanshan Xu |
| author_sort | Yuan Jiang |
| collection | DOAJ |
| description | Aim. To investigate the effect of photodynamic action of LED-activated curcumin on cell viability, membrane permeability, and intracellular reactive oxygen species of Staphylococcus aureus. Methods. Staphylococcus aureus was incubated with the different concentrations of curcumin for 60 min and then irradiated by blue light with the wavelength of 470 nm and with light dose of 3 J/cm2. The colony forming unit assay was used to investigate photocytotoxicity of curcumin on Staphylococcus aureus, confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) for assaying membrane permeability, FCM analysis with DCFH-DA staining for measuring the intracellular ROS level, and transmission electron microscopy (TEM) for observing morphology and structure. Results. Blue light-activated curcumin significantly killed Staphylococcus aureus in a curcumin dose-dependent manner. TEM observed remarkable structural damages in S. aureus after light-activated curcumin. More red fluorescence of PI dye was found in S. aureus treated by blue light-activated curcumin than in those of the controlled bacterial cells. Intracellular ROS increase was observed after light-activated curcumin. Conclusion. Blue light-activated curcumin markedly damaged membrane permeability, resulting in cell death of Staphylococcus aureus and highlighted that intracellular ROS increase might be an important event in photodynamic killing of Staphylococcus aureus in the presence of curcumin. |
| format | Article |
| id | doaj-art-2706a2c552fb48b9b3f709d9e7689d68 |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-2706a2c552fb48b9b3f709d9e7689d682025-02-03T05:59:54ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/637601637601Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane DamageYuan Jiang0Albert Wingnang Leung1Heyu Hua2Xiancai Rao3Chuanshan Xu4Department of Rehabilitation Medicine, Second Affiliated Hospital, Chongqing Medical College, Chongqing 400010, ChinaSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongDepartment of Microbiology, College of Basic Medical Science, Third Military Medical University, Chongqing 400038, ChinaSchool of Chinese Medicine, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong KongAim. To investigate the effect of photodynamic action of LED-activated curcumin on cell viability, membrane permeability, and intracellular reactive oxygen species of Staphylococcus aureus. Methods. Staphylococcus aureus was incubated with the different concentrations of curcumin for 60 min and then irradiated by blue light with the wavelength of 470 nm and with light dose of 3 J/cm2. The colony forming unit assay was used to investigate photocytotoxicity of curcumin on Staphylococcus aureus, confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) for assaying membrane permeability, FCM analysis with DCFH-DA staining for measuring the intracellular ROS level, and transmission electron microscopy (TEM) for observing morphology and structure. Results. Blue light-activated curcumin significantly killed Staphylococcus aureus in a curcumin dose-dependent manner. TEM observed remarkable structural damages in S. aureus after light-activated curcumin. More red fluorescence of PI dye was found in S. aureus treated by blue light-activated curcumin than in those of the controlled bacterial cells. Intracellular ROS increase was observed after light-activated curcumin. Conclusion. Blue light-activated curcumin markedly damaged membrane permeability, resulting in cell death of Staphylococcus aureus and highlighted that intracellular ROS increase might be an important event in photodynamic killing of Staphylococcus aureus in the presence of curcumin.http://dx.doi.org/10.1155/2014/637601 |
| spellingShingle | Yuan Jiang Albert Wingnang Leung Heyu Hua Xiancai Rao Chuanshan Xu Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage International Journal of Photoenergy |
| title | Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage |
| title_full | Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage |
| title_fullStr | Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage |
| title_full_unstemmed | Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage |
| title_short | Photodynamic Action of LED-Activated Curcumin against Staphylococcus aureus Involving Intracellular ROS Increase and Membrane Damage |
| title_sort | photodynamic action of led activated curcumin against staphylococcus aureus involving intracellular ros increase and membrane damage |
| url | http://dx.doi.org/10.1155/2014/637601 |
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