Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules
Xin-Rong He,1,* Jia-Min Song,1,* Jia-Peng Zhao,2 Jing Zhang,1 Jing-Tao Feng,1 Shu-Qin Chen,3 Zhi-Yuan Zhou,1 Hong-Ming Wang,4 Yue Zhang,5 Ya Feng,1 Yun-Cheng Wu,1 Xiao-Ying Zhu1 1Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medic...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-06-01
|
| Series: | Nature and Science of Sleep |
| Subjects: | |
| Online Access: | https://www.dovepress.com/transcriptomic-changes-and-pathological-mechanisms-in-familial-and-spo-peer-reviewed-fulltext-article-NSS |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849417958715031552 |
|---|---|
| author | He XR Song JM Zhao JP Zhang J Feng JT Chen SQ Zhou ZY Wang HM Zhang Y Feng Y Wu YC Zhu XY |
| author_facet | He XR Song JM Zhao JP Zhang J Feng JT Chen SQ Zhou ZY Wang HM Zhang Y Feng Y Wu YC Zhu XY |
| author_sort | He XR |
| collection | DOAJ |
| description | Xin-Rong He,1,* Jia-Min Song,1,* Jia-Peng Zhao,2 Jing Zhang,1 Jing-Tao Feng,1 Shu-Qin Chen,3 Zhi-Yuan Zhou,1 Hong-Ming Wang,4 Yue Zhang,5 Ya Feng,1 Yun-Cheng Wu,1 Xiao-Ying Zhu1 1Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China; 2Department of Orthopedics, Yongxiu County Traditional Chinese Medicine Hospital in Jiujiang City, Jiangxi, 330304, People’s Republic of China; 3Community Health Service Center, Ouyang Road District, Hongkou District, Shanghai, 200080, People’s Republic of China; 4Department of Clinical Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China; 5Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yun-Cheng Wu, Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China, Tel +86-21-63240090, Email drwu2006@163.com Xiao-Ying Zhu, Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China, Email docxiaoying@163.comPurpose: Individuals affected by restless legs syndrome (RLS) tend to have familial predispositions without fully explained by genetic variants, and transcriptomic analysis may help elucidate the pathogenic mechanisms of RLS. The study aims to investigate transcriptomic changes and underlying pathological mechanisms in familial and sporadic idiopathic RLS to uncover potential contributors to its pathogenesis.Patients and Methods: This study included 37 RLS patients, 39 unrelated healthy controls and 19 healthy relatives of RLS patients with a positive family history. Messenger RNA (mRNA) extracted from the peripheral blood mononuclear cells of these participants was analyzed via next-generation sequencing, followed by GO and KEGG pathway analyses. Differentially expressed mRNAs were validated by RT-qPCR in a subset of patients and controls. The relationships between the expression levels and clinical indices were evaluated via correlation analysis.Results: After comparing with unrelated healthy controls and excluding genes with similar expression patterns in familial healthy controls, we identified nine upregulated and 28 downregulated mRNAs specifically in RLS patients. GO enrichment analysis indicated that these mRNAs are involved in protein binding and catalytic activity. KEGG analysis revealed that inflammation-related signaling pathways and cell adhesion molecules (CAMs) may be associated with RLS. Three specific mRNAs, including SPARCL1, CCL8 and SELE, demonstrated notably downregulated expression in RLS patients and were subsequently validated in a subset of 10 patients and 19 healthy controls.Conclusion: This study revealed differentially expressed SPARCL1, CCL8 and SELE in RLS patients, indicating the potential involvement of inflammatory pathways and CAMs in RLS pathogenesis. These findings further support the association between RLS, inflammation, and synaptic transmission, providing insights into potential diagnostic and therapeutic strategies targeting these pathways.Keywords: restless legs syndrome, peripheral blood mononuclear cells, bioinformatics, transcriptomics, inflammation, message-RNA sequencing |
| format | Article |
| id | doaj-art-26fa89372eaa4a6a9336de534caefb6d |
| institution | Kabale University |
| issn | 1179-1608 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Nature and Science of Sleep |
| spelling | doaj-art-26fa89372eaa4a6a9336de534caefb6d2025-08-20T03:32:36ZengDove Medical PressNature and Science of Sleep1179-16082025-06-01Volume 17Issue 112311247103794Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion MoleculesHe XR0Song JM1Zhao JP2Zhang J3Feng JT4Chen SQ5Zhou ZY6Wang HM7Zhang Y8Feng Y9Wu YC10Zhu XY11Department of NeurologyDepartment of NeurologyDepartment of OrthopedicsDepartment of NeurologyDepartment of NeurologyDepartment of NeurologyDepartment of NeurologyDepartment of Clinical Laboratory MedicineDepartment of Bioinformatics and BiostatisticsDepartment of NeurologyDepartment of NeurologyDepartment of NeurologyXin-Rong He,1,* Jia-Min Song,1,* Jia-Peng Zhao,2 Jing Zhang,1 Jing-Tao Feng,1 Shu-Qin Chen,3 Zhi-Yuan Zhou,1 Hong-Ming Wang,4 Yue Zhang,5 Ya Feng,1 Yun-Cheng Wu,1 Xiao-Ying Zhu1 1Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China; 2Department of Orthopedics, Yongxiu County Traditional Chinese Medicine Hospital in Jiujiang City, Jiangxi, 330304, People’s Republic of China; 3Community Health Service Center, Ouyang Road District, Hongkou District, Shanghai, 200080, People’s Republic of China; 4Department of Clinical Laboratory Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China; 5Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yun-Cheng Wu, Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China, Tel +86-21-63240090, Email drwu2006@163.com Xiao-Ying Zhu, Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, People’s Republic of China, Email docxiaoying@163.comPurpose: Individuals affected by restless legs syndrome (RLS) tend to have familial predispositions without fully explained by genetic variants, and transcriptomic analysis may help elucidate the pathogenic mechanisms of RLS. The study aims to investigate transcriptomic changes and underlying pathological mechanisms in familial and sporadic idiopathic RLS to uncover potential contributors to its pathogenesis.Patients and Methods: This study included 37 RLS patients, 39 unrelated healthy controls and 19 healthy relatives of RLS patients with a positive family history. Messenger RNA (mRNA) extracted from the peripheral blood mononuclear cells of these participants was analyzed via next-generation sequencing, followed by GO and KEGG pathway analyses. Differentially expressed mRNAs were validated by RT-qPCR in a subset of patients and controls. The relationships between the expression levels and clinical indices were evaluated via correlation analysis.Results: After comparing with unrelated healthy controls and excluding genes with similar expression patterns in familial healthy controls, we identified nine upregulated and 28 downregulated mRNAs specifically in RLS patients. GO enrichment analysis indicated that these mRNAs are involved in protein binding and catalytic activity. KEGG analysis revealed that inflammation-related signaling pathways and cell adhesion molecules (CAMs) may be associated with RLS. Three specific mRNAs, including SPARCL1, CCL8 and SELE, demonstrated notably downregulated expression in RLS patients and were subsequently validated in a subset of 10 patients and 19 healthy controls.Conclusion: This study revealed differentially expressed SPARCL1, CCL8 and SELE in RLS patients, indicating the potential involvement of inflammatory pathways and CAMs in RLS pathogenesis. These findings further support the association between RLS, inflammation, and synaptic transmission, providing insights into potential diagnostic and therapeutic strategies targeting these pathways.Keywords: restless legs syndrome, peripheral blood mononuclear cells, bioinformatics, transcriptomics, inflammation, message-RNA sequencinghttps://www.dovepress.com/transcriptomic-changes-and-pathological-mechanisms-in-familial-and-spo-peer-reviewed-fulltext-article-NSSRestless legs syndromePeripheral blood mononuclear cellsBioinformaticsTranscriptomicsInflammationMessage-RNA sequencing |
| spellingShingle | He XR Song JM Zhao JP Zhang J Feng JT Chen SQ Zhou ZY Wang HM Zhang Y Feng Y Wu YC Zhu XY Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules Nature and Science of Sleep Restless legs syndrome Peripheral blood mononuclear cells Bioinformatics Transcriptomics Inflammation Message-RNA sequencing |
| title | Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules |
| title_full | Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules |
| title_fullStr | Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules |
| title_full_unstemmed | Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules |
| title_short | Transcriptomic Changes and Pathological Mechanisms in Familial and Sporadic Idiopathic Restless Legs Syndrome: Implications for Inflammation and Cell Adhesion Molecules |
| title_sort | transcriptomic changes and pathological mechanisms in familial and sporadic idiopathic restless legs syndrome implications for inflammation and cell adhesion molecules |
| topic | Restless legs syndrome Peripheral blood mononuclear cells Bioinformatics Transcriptomics Inflammation Message-RNA sequencing |
| url | https://www.dovepress.com/transcriptomic-changes-and-pathological-mechanisms-in-familial-and-spo-peer-reviewed-fulltext-article-NSS |
| work_keys_str_mv | AT hexr transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT songjm transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT zhaojp transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT zhangj transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT fengjt transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT chensq transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT zhouzy transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT wanghm transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT zhangy transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT fengy transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT wuyc transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules AT zhuxy transcriptomicchangesandpathologicalmechanismsinfamilialandsporadicidiopathicrestlesslegssyndromeimplicationsforinflammationandcelladhesionmolecules |