A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

This paper presents a LAMP- (loop-mediated isothermal amplification-) based lab-on-disk optical system that allows the simultaneous detection of hepatitis B virus, hepatitis C virus, and cytomegalovirus. The various flow stages are controlled in the proposed system using different balance among cent...

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Main Authors: Hung-Cheng Chang, Yu-Tin Chao, Jia-Yush Yen, Ya-Lin Yu, Chun-Nan Lee, Bing-Ching Ho, Kou-Chen Liu, Jiunn Fang, Chii-Wann Lin, Jiun-Haw Lee
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Advances in Materials Science and Engineering
Online Access:http://dx.doi.org/10.1155/2015/306708
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author Hung-Cheng Chang
Yu-Tin Chao
Jia-Yush Yen
Ya-Lin Yu
Chun-Nan Lee
Bing-Ching Ho
Kou-Chen Liu
Jiunn Fang
Chii-Wann Lin
Jiun-Haw Lee
author_facet Hung-Cheng Chang
Yu-Tin Chao
Jia-Yush Yen
Ya-Lin Yu
Chun-Nan Lee
Bing-Ching Ho
Kou-Chen Liu
Jiunn Fang
Chii-Wann Lin
Jiun-Haw Lee
author_sort Hung-Cheng Chang
collection DOAJ
description This paper presents a LAMP- (loop-mediated isothermal amplification-) based lab-on-disk optical system that allows the simultaneous detection of hepatitis B virus, hepatitis C virus, and cytomegalovirus. The various flow stages are controlled in the proposed system using different balance among centrifugal pumping, Coriolis pumping, and the capillary force. We have implemented a servo system for positioning and speed control for the heating and centrifugal pumping. We have also successfully employed a polymer light-emitting diode section for turbidity detection. The easy-to-use one-click system can perform diagnostics in less than 1 hour.
format Article
id doaj-art-26ebda8b5a784898b90c4dfc7f7de60e
institution OA Journals
issn 1687-8434
1687-8442
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Advances in Materials Science and Engineering
spelling doaj-art-26ebda8b5a784898b90c4dfc7f7de60e2025-08-20T02:04:23ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422015-01-01201510.1155/2015/306708306708A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMVHung-Cheng Chang0Yu-Tin Chao1Jia-Yush Yen2Ya-Lin Yu3Chun-Nan Lee4Bing-Ching Ho5Kou-Chen Liu6Jiunn Fang7Chii-Wann Lin8Jiun-Haw Lee9Department of Mechanical Engineering, National Taiwan University, Taipei 106, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 106, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 106, TaiwanDepartment of Mechanical Engineering, National Taiwan University, Taipei 106, TaiwanCollege of Medicine, National Taiwan University, Taipei 106, TaiwanCollege of Medicine, National Taiwan University, Taipei 106, TaiwanDepartment of Electronic Engineering, Chang Gung University, Taoyuan 333, TaiwanDepartment of Aerospace and System Engineering, Feng Chia University, Taichung 407, TaiwanInstitute of Biomedical Engineering, National Taiwan University, Taipei 106, TaiwanDepartment of Electronic Engineering, National Taiwan University, Taipei 106, TaiwanThis paper presents a LAMP- (loop-mediated isothermal amplification-) based lab-on-disk optical system that allows the simultaneous detection of hepatitis B virus, hepatitis C virus, and cytomegalovirus. The various flow stages are controlled in the proposed system using different balance among centrifugal pumping, Coriolis pumping, and the capillary force. We have implemented a servo system for positioning and speed control for the heating and centrifugal pumping. We have also successfully employed a polymer light-emitting diode section for turbidity detection. The easy-to-use one-click system can perform diagnostics in less than 1 hour.http://dx.doi.org/10.1155/2015/306708
spellingShingle Hung-Cheng Chang
Yu-Tin Chao
Jia-Yush Yen
Ya-Lin Yu
Chun-Nan Lee
Bing-Ching Ho
Kou-Chen Liu
Jiunn Fang
Chii-Wann Lin
Jiun-Haw Lee
A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
Advances in Materials Science and Engineering
title A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
title_full A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
title_fullStr A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
title_full_unstemmed A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
title_short A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV
title_sort turbidity test based centrifugal microfluidics diagnostic system for simultaneous detection of hbv hcv and cmv
url http://dx.doi.org/10.1155/2015/306708
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