SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING
Aim. To compare structural and tensile properties of electrospun vascular grafts fabricated of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen solution utilizing either joint or separate feed of these polymers, and to further evaluate adhesion and viabili...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State Medical University
2017-12-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/64 |
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| author | ELENA A. Velikanova LARISA V. Antonova VICTORIA V. Sevostyanova ANTON G. Kutikhin TATIANA V. Glushkova VERA G. Matveeva EVGENIYA O. Krivkina OLGA L. Barbarash LEONID S. Barbarash |
| author_facet | ELENA A. Velikanova LARISA V. Antonova VICTORIA V. Sevostyanova ANTON G. Kutikhin TATIANA V. Glushkova VERA G. Matveeva EVGENIYA O. Krivkina OLGA L. Barbarash LEONID S. Barbarash |
| author_sort | ELENA A. Velikanova |
| collection | DOAJ |
| description | Aim. To compare structural and tensile properties of electrospun vascular grafts fabricated of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen solution utilizing either joint or separate feed of these polymers, and to further evaluate adhesion and viability of endothelial cells on both types of the grafts. Materials and Methods. Either 4 mm or 8 mm diameter electrospun grafts were assessed by scanning electron microscopy and uniaxial tension test following culture of human umbilical vein endothelial cells on the graft surface, either Hoechst 33342/PKH26 or acridine orange/ethidium bromide staining, and fluorescence microscopy. Results. Grafts fabricated using the separate feed had poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen located in core and sheath of electrospun fibers, respectively. This two-layer structure of fibers resulted in 5-fold increase in adhesion and 1.5-fold increase in viability of endothelial cells cultured on the graft surface; therefore, the total number of viable endothelial cells increased more than 8-fold. Both types of the grafts demonstrated higher durability and elasticity compared to internal mammary artery, a widely applied vascular conduit. Conclusion. Separate feed of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) blend and type I collagen solution significantly increases the biocompatibility of the electrospun vascular grafts while not affecting their mechanical competence. |
| format | Article |
| id | doaj-art-26e79102b88c4de4a4cde69255ee89eb |
| institution | DOAJ |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2017-12-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-26e79102b88c4de4a4cde69255ee89eb2025-08-20T02:49:09ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412017-12-012461363SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTINGELENA A. Velikanova0LARISA V. Antonova1VICTORIA V. Sevostyanova2ANTON G. Kutikhin3TATIANA V. Glushkova4VERA G. Matveeva5EVGENIYA O. Krivkina6OLGA L. Barbarash7LEONID S. Barbarash8Research Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesResearch Institute for Complex Issues of Cardiovascular DiseasesAim. To compare structural and tensile properties of electrospun vascular grafts fabricated of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen solution utilizing either joint or separate feed of these polymers, and to further evaluate adhesion and viability of endothelial cells on both types of the grafts. Materials and Methods. Either 4 mm or 8 mm diameter electrospun grafts were assessed by scanning electron microscopy and uniaxial tension test following culture of human umbilical vein endothelial cells on the graft surface, either Hoechst 33342/PKH26 or acridine orange/ethidium bromide staining, and fluorescence microscopy. Results. Grafts fabricated using the separate feed had poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/ poly(ε-caprolactone) blend and type I collagen located in core and sheath of electrospun fibers, respectively. This two-layer structure of fibers resulted in 5-fold increase in adhesion and 1.5-fold increase in viability of endothelial cells cultured on the graft surface; therefore, the total number of viable endothelial cells increased more than 8-fold. Both types of the grafts demonstrated higher durability and elasticity compared to internal mammary artery, a widely applied vascular conduit. Conclusion. Separate feed of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) blend and type I collagen solution significantly increases the biocompatibility of the electrospun vascular grafts while not affecting their mechanical competence.https://fcm.kemsmu.ru/jour/article/view/64сосудистые графтыколлаген i типаэлектроспиннингбиосовместимостьэндотелиальные клеткиvascular graftstype i collagenelectrospinningbiocompatibilityendothelial cells |
| spellingShingle | ELENA A. Velikanova LARISA V. Antonova VICTORIA V. Sevostyanova ANTON G. Kutikhin TATIANA V. Glushkova VERA G. Matveeva EVGENIYA O. Krivkina OLGA L. Barbarash LEONID S. Barbarash SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING Фундаментальная и клиническая медицина сосудистые графты коллаген i типа электроспиннинг биосовместимость эндотелиальные клетки vascular grafts type i collagen electrospinning biocompatibility endothelial cells |
| title | SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING |
| title_full | SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING |
| title_fullStr | SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING |
| title_full_unstemmed | SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING |
| title_short | SEPARATE FEED OF TYPE I COLLAGEN SOLUTION AND POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)/POLY(ε-CAPROLACTONE) BLEND DURING ELECTROSPINNING INCREASES BIOCOMPATIBILITY OF VASCULAR GRAFTS: IN VITRO TESTING |
| title_sort | separate feed of type i collagen solution and poly 3 hydroxybutyrate co 3 hydroxyvalerate poly ε caprolactone blend during electrospinning increases biocompatibility of vascular grafts in vitro testing |
| topic | сосудистые графты коллаген i типа электроспиннинг биосовместимость эндотелиальные клетки vascular grafts type i collagen electrospinning biocompatibility endothelial cells |
| url | https://fcm.kemsmu.ru/jour/article/view/64 |
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