Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway
Background. To study the effects of z-guggulsterone on gastric cancer cell apoptosis and the mechanism related. Materials and Methods. Human gastric tumor SGC-7901 cells and GES-1 normal epithelial cells were treated with z-guggulsterone (0–75 μM) for 24 h. MTT assay was applied to evaluate cell pro...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2021/3152304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567359822364672 |
|---|---|
| author | Ruxi Lv Min Zhu Kun Chen Haitao Xie Hongxia Bai Qingfa Chen |
| author_facet | Ruxi Lv Min Zhu Kun Chen Haitao Xie Hongxia Bai Qingfa Chen |
| author_sort | Ruxi Lv |
| collection | DOAJ |
| description | Background. To study the effects of z-guggulsterone on gastric cancer cell apoptosis and the mechanism related. Materials and Methods. Human gastric tumor SGC-7901 cells and GES-1 normal epithelial cells were treated with z-guggulsterone (0–75 μM) for 24 h. MTT assay was applied to evaluate cell proliferation. Flow cytometry and Hoechst staining were used to assess cell apoptosis. Western blotting was applied to evaluate FXR, small heterodimer partner (SHP), Bcl-2, and Bax protein expression. ELISA was applied to gain the levels of active caspase-3 and the contents of TNF-α, TGF-β1, and VEGF. Results. The expression levels of FXR and SHP were higher in tumor cells than in normal epithelial cells. Inhibition of FXR signaling with z-guggulsterone dose-dependently inhibited SGC-7901 cell proliferation and promoted SGC-7901 cell apoptosis. Bcl-2 protein expression was significantly decreased, and active caspase-3 and Bax protein expression was increased in SGC-7901 cells incubated with z-guggulsterone. The content of TNF-α was significantly increased, and the contents of VEGF and TGF-β1 were decreased in SGC-7901 cells incubated with z-guggulsterone. Conclusions. Inhibition of FXR signaling with z-guggulsterone induced anticancer effects in SGC-7901 cells by decreasing cell proliferation and promoting apoptosis. Z-guggulsterone induced cell apoptosis through the mitochondria-dependent pathway. |
| format | Article |
| id | doaj-art-26db762ef49146258bb439ffaa49cfc1 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-26db762ef49146258bb439ffaa49cfc12025-02-03T01:01:43ZengWileyThe Scientific World Journal2356-61401537-744X2021-01-01202110.1155/2021/31523043152304Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent PathwayRuxi Lv0Min Zhu1Kun Chen2Haitao Xie3Hongxia Bai4Qingfa Chen5Department of Gastroenterology, Liaocheng People’s Hospital, Liaocheng 252000, ChinaClinical Laboratory, Liaocheng People’s Hospital, Liaocheng 252000, ChinaCollege of Medicine, Liaocheng University, Liaocheng 252000, ChinaCentre for Research, Xiankangda Bio-Tech Corporation, Dongguan 523000, ChinaDepartment of Gastroenterology, Liaocheng People’s Hospital, Liaocheng 252000, ChinaThe Institute for Tissue Engineering and Regenerative Medicine, The Liaocheng University/Liaocheng People’s Hospital, Liaocheng 252000, ChinaBackground. To study the effects of z-guggulsterone on gastric cancer cell apoptosis and the mechanism related. Materials and Methods. Human gastric tumor SGC-7901 cells and GES-1 normal epithelial cells were treated with z-guggulsterone (0–75 μM) for 24 h. MTT assay was applied to evaluate cell proliferation. Flow cytometry and Hoechst staining were used to assess cell apoptosis. Western blotting was applied to evaluate FXR, small heterodimer partner (SHP), Bcl-2, and Bax protein expression. ELISA was applied to gain the levels of active caspase-3 and the contents of TNF-α, TGF-β1, and VEGF. Results. The expression levels of FXR and SHP were higher in tumor cells than in normal epithelial cells. Inhibition of FXR signaling with z-guggulsterone dose-dependently inhibited SGC-7901 cell proliferation and promoted SGC-7901 cell apoptosis. Bcl-2 protein expression was significantly decreased, and active caspase-3 and Bax protein expression was increased in SGC-7901 cells incubated with z-guggulsterone. The content of TNF-α was significantly increased, and the contents of VEGF and TGF-β1 were decreased in SGC-7901 cells incubated with z-guggulsterone. Conclusions. Inhibition of FXR signaling with z-guggulsterone induced anticancer effects in SGC-7901 cells by decreasing cell proliferation and promoting apoptosis. Z-guggulsterone induced cell apoptosis through the mitochondria-dependent pathway.http://dx.doi.org/10.1155/2021/3152304 |
| spellingShingle | Ruxi Lv Min Zhu Kun Chen Haitao Xie Hongxia Bai Qingfa Chen Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway The Scientific World Journal |
| title | Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway |
| title_full | Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway |
| title_fullStr | Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway |
| title_full_unstemmed | Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway |
| title_short | Z-Guggulsterone Induces Apoptosis in Gastric Cancer Cells through the Intrinsic Mitochondria-Dependent Pathway |
| title_sort | z guggulsterone induces apoptosis in gastric cancer cells through the intrinsic mitochondria dependent pathway |
| url | http://dx.doi.org/10.1155/2021/3152304 |
| work_keys_str_mv | AT ruxilv zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway AT minzhu zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway AT kunchen zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway AT haitaoxie zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway AT hongxiabai zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway AT qingfachen zguggulsteroneinducesapoptosisingastriccancercellsthroughtheintrinsicmitochondriadependentpathway |