Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells
Differentiation of stem cells to Schwann is considered efficient way for nerve regeneration since the sources of human Schwann cells are limited for clinical application. It is demonstrated that mimicking micromechanical forces or micro/nanotopographical environments that stem cells are experienced...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1586718 |
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| author | Mehrdad Moosazadeh Moghaddam Shahin Bonakdar Mohammad Ali Shokrgozar Arash Zaminy Hojatollah Vali Shahab Faghihi |
| author_facet | Mehrdad Moosazadeh Moghaddam Shahin Bonakdar Mohammad Ali Shokrgozar Arash Zaminy Hojatollah Vali Shahab Faghihi |
| author_sort | Mehrdad Moosazadeh Moghaddam |
| collection | DOAJ |
| description | Differentiation of stem cells to Schwann is considered efficient way for nerve regeneration since the sources of human Schwann cells are limited for clinical application. It is demonstrated that mimicking micromechanical forces or micro/nanotopographical environments that stem cells are experienced in vivo could control their fate. Here, the potency of substrates with imprinted cell-like topographies for direct differentiation of adipose-derived mesenchymal stem cells (ADSCs) into Schwann cells (SCs) is reported. For the preparation of substrates with imprinted SC-Like topographies, SCs are isolated from the sciatic nerve, grown, fixed, and then SC morphologies are transferred to polydimethylsiloxane (PDMS) substrates by mold casting. Subsequently, mesenchymal stem cells (MSCs) are seeded on the SC-imprinted substrates and their differentiation to SCs is evaluated by immunocytochemistry, real-time PCR, and western blotting. Analysis of morphology and expression of SC-specific markers show that MSCs cultured on the imprinted substrates have the typical SC-like morphology and express SC-specific markers including S100b, p75NTR, and Sox10. It is believed that specific cell-like topographies and related micromechanical cues can be sufficient for direct differentiation of ADSCs into Schwann cells by cell-imprinting method as a physical technique. |
| format | Article |
| id | doaj-art-26d17ffeee8b47dfbd86af42c8f35400 |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-26d17ffeee8b47dfbd86af42c8f354002025-08-20T03:51:19ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711022103510.1080/21691401.2019.1586718Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cellsMehrdad Moosazadeh Moghaddam0Shahin Bonakdar1Mohammad Ali Shokrgozar2Arash Zaminy3Hojatollah Vali4Shahab Faghihi5Stem Cell and Regenerative Medicine Group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranNational Cell Bank, Pasteur Institute of Iran, Tehran, IranNational Cell Bank, Pasteur Institute of Iran, Tehran, IranNeuroscience Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, IranDepartment of Anatomy and Cell Biology, McGill University, Montréal, QC, CanadaStem Cell and Regenerative Medicine Group, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, IranDifferentiation of stem cells to Schwann is considered efficient way for nerve regeneration since the sources of human Schwann cells are limited for clinical application. It is demonstrated that mimicking micromechanical forces or micro/nanotopographical environments that stem cells are experienced in vivo could control their fate. Here, the potency of substrates with imprinted cell-like topographies for direct differentiation of adipose-derived mesenchymal stem cells (ADSCs) into Schwann cells (SCs) is reported. For the preparation of substrates with imprinted SC-Like topographies, SCs are isolated from the sciatic nerve, grown, fixed, and then SC morphologies are transferred to polydimethylsiloxane (PDMS) substrates by mold casting. Subsequently, mesenchymal stem cells (MSCs) are seeded on the SC-imprinted substrates and their differentiation to SCs is evaluated by immunocytochemistry, real-time PCR, and western blotting. Analysis of morphology and expression of SC-specific markers show that MSCs cultured on the imprinted substrates have the typical SC-like morphology and express SC-specific markers including S100b, p75NTR, and Sox10. It is believed that specific cell-like topographies and related micromechanical cues can be sufficient for direct differentiation of ADSCs into Schwann cells by cell-imprinting method as a physical technique.https://www.tandfonline.com/doi/10.1080/21691401.2019.1586718Mesenchymal stem cellSchwann cellcell-like topographyimprinted substratedirect differentiation |
| spellingShingle | Mehrdad Moosazadeh Moghaddam Shahin Bonakdar Mohammad Ali Shokrgozar Arash Zaminy Hojatollah Vali Shahab Faghihi Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells Artificial Cells, Nanomedicine, and Biotechnology Mesenchymal stem cell Schwann cell cell-like topography imprinted substrate direct differentiation |
| title | Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells |
| title_full | Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells |
| title_fullStr | Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells |
| title_full_unstemmed | Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells |
| title_short | Engineered substrates with imprinted cell-like topographies induce direct differentiation of adipose-derived mesenchymal stem cells into Schwann cells |
| title_sort | engineered substrates with imprinted cell like topographies induce direct differentiation of adipose derived mesenchymal stem cells into schwann cells |
| topic | Mesenchymal stem cell Schwann cell cell-like topography imprinted substrate direct differentiation |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1586718 |
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