Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats
ABSTRACT Objective: This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats. Materials and methods: The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione p...
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Brazilian Society of Endocrinology and Metabolism
2025-01-01
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| Series: | Archives of Endocrinology and Metabolism |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000306&lng=en&tlng=en |
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| author | Jessica de Freitas Nascimento Keciany Alves de Oliveira Paula Alexandre de Freitas Júlia de Araújo Marques Falci Renata Prado Vasconcelos Saulo Chaves Magalhães Talita Mendes Farias Maria Isabel Cardoso Alonso-Vale Adriano Cesar Carneiro Loureiro Denise Pires de Carvalho Rodrigo Soares Fortunato Ariclécio Cunha de Oliveira |
| author_facet | Jessica de Freitas Nascimento Keciany Alves de Oliveira Paula Alexandre de Freitas Júlia de Araújo Marques Falci Renata Prado Vasconcelos Saulo Chaves Magalhães Talita Mendes Farias Maria Isabel Cardoso Alonso-Vale Adriano Cesar Carneiro Loureiro Denise Pires de Carvalho Rodrigo Soares Fortunato Ariclécio Cunha de Oliveira |
| author_sort | Jessica de Freitas Nascimento |
| collection | DOAJ |
| description | ABSTRACT Objective: This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats. Materials and methods: The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, along with the production of NADPH oxidases dependent on H2O2 and gene expression of NOX1, NOX2, and NOX4. Results: The retroperitoneal fat pad in males compared with females had greater NOX2 and NOX4 expression, along with higher superoxide dismutase activity. Additionally, their subcutaneous fat pad had greater NOX4 expression and higher intracellular H2O2 production, together with greater expression and activity of both superoxide dismutase and catalase. Conclusion: The white adipose tissue of male rats had greater reactive oxygen species (ROS) production compared with that of female rats, but also a proportionally greater antioxidant response. These findings are important for ongoing investigations into how sex differences may be linked to the development of metabolic diseases and the unique susceptibilities of each sex. |
| format | Article |
| id | doaj-art-26cc4105cfe04b638363872d0719d123 |
| institution | Kabale University |
| issn | 2359-4292 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Brazilian Society of Endocrinology and Metabolism |
| record_format | Article |
| series | Archives of Endocrinology and Metabolism |
| spelling | doaj-art-26cc4105cfe04b638363872d0719d1232025-01-21T07:45:35ZengBrazilian Society of Endocrinology and MetabolismArchives of Endocrinology and Metabolism2359-42922025-01-0168spe110.20945/2359-4292-2024-0136Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male ratsJessica de Freitas Nascimentohttps://orcid.org/0009-0007-9960-2990Keciany Alves de Oliveirahttps://orcid.org/0000-0002-9928-3826Paula Alexandre de Freitashttps://orcid.org/0000-0003-1791-280XJúlia de Araújo Marques Falcihttps://orcid.org/0009-0008-1631-9039Renata Prado Vasconceloshttps://orcid.org/0000-0002-0586-126XSaulo Chaves Magalhãeshttps://orcid.org/0000-0001-9224-0850Talita Mendes Fariashttps://orcid.org/0000-0002-3307-2735Maria Isabel Cardoso Alonso-Valehttps://orcid.org/0000-0001-5847-8080Adriano Cesar Carneiro Loureirohttps://orcid.org/0000-0002-9681-1945Denise Pires de Carvalhohttps://orcid.org/0000-0001-6933-6424Rodrigo Soares Fortunatohttps://orcid.org/0000-0003-3497-8173Ariclécio Cunha de Oliveirahttps://orcid.org/0000-0002-9295-8157ABSTRACT Objective: This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats. Materials and methods: The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, along with the production of NADPH oxidases dependent on H2O2 and gene expression of NOX1, NOX2, and NOX4. Results: The retroperitoneal fat pad in males compared with females had greater NOX2 and NOX4 expression, along with higher superoxide dismutase activity. Additionally, their subcutaneous fat pad had greater NOX4 expression and higher intracellular H2O2 production, together with greater expression and activity of both superoxide dismutase and catalase. Conclusion: The white adipose tissue of male rats had greater reactive oxygen species (ROS) production compared with that of female rats, but also a proportionally greater antioxidant response. These findings are important for ongoing investigations into how sex differences may be linked to the development of metabolic diseases and the unique susceptibilities of each sex.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000306&lng=en&tlng=enAdipose tissuesexual dimorphismredox homeostasisNADPH oxidase |
| spellingShingle | Jessica de Freitas Nascimento Keciany Alves de Oliveira Paula Alexandre de Freitas Júlia de Araújo Marques Falci Renata Prado Vasconcelos Saulo Chaves Magalhães Talita Mendes Farias Maria Isabel Cardoso Alonso-Vale Adriano Cesar Carneiro Loureiro Denise Pires de Carvalho Rodrigo Soares Fortunato Ariclécio Cunha de Oliveira Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats Archives of Endocrinology and Metabolism Adipose tissue sexual dimorphism redox homeostasis NADPH oxidase |
| title | Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| title_full | Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| title_fullStr | Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| title_full_unstemmed | Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| title_short | Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| title_sort | increased nox dependent ros production and proportionally enhanced antioxidant response in white adipose tissue of male rats |
| topic | Adipose tissue sexual dimorphism redox homeostasis NADPH oxidase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2359-39972024001000306&lng=en&tlng=en |
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