THE EFFECT OF DECELLULARIZATION TECHNIQUE ON COLLAGEN TYPE II AND MATRICES POROSITY OF CARTILAGE BOVINE SCAFFOLD
Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of t...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2019-12-01
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| Series: | Journal Orthopaedi and Traumatology Surabaya |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/9268 |
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| Summary: | Background: Cartilage has limited capacity for self-repair in case of defect. Stem cell based tissue engineering has become an alternative therapy for the treatment of cartilage defect. The use of decellularized cartilage bovine can serve as scaffold to support proliferation dan differentiation of the stem cell.
Purpose: This research is to compare the effect of decellularization technique on collagen type II and matrices porosity of cartilage bovine scaffold.
Methods: This experimental laboratory research used cartilage bovine scaffold which was decellularized using physical, chemical and enzymatic techniques with different time and concentration variations, then collagen type II measurement was performed by immunohistochemical method and evaluation of porosity size with electron microscopy.
Results: In the preparations with the highest concentration and the longest immersion time of 5% SDS for 72 hours, the decellularization process occurs perfectly. The collagen type II content was found to have significantly higher concentrations than the controls and other preparations, and have interconnected porosities in large size.
Conclusion: High concentrations of collagen type II and large diameter of pores after decellularization process in cartilage bovine scaffold confirm that this scaffold still has optimum biochemical cues that can be a good mechanical framework and microenvironment stem cell in effort to repair the cartilage defects. This result was expected to become a standard for the decellularization making of cartilage bovine scaffold. |
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| ISSN: | 2722-712X 2460-8742 |