<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types

Background. Vigna unguiculata (L.) Walp. is among important legume crops. Agricultural producers prefer cultivars suitable for mechanized cultivation, with a determinate growth habit type. Plant architectonics depends on the functioning of the apical meristem, while the transition to the reproductiv...

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Main Authors: E. A. Krylova, E. K. Khlestkina
Format: Article
Language:English
Published: N.I. Vavilov All-Russian Institute of Plant Genetic Resources 2025-01-01
Series:Труды по прикладной ботанике, генетике и селекции
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Online Access:https://elpub.vir.nw.ru/jour/article/view/2119
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author E. A. Krylova
E. K. Khlestkina
author_facet E. A. Krylova
E. K. Khlestkina
author_sort E. A. Krylova
collection DOAJ
description Background. Vigna unguiculata (L.) Walp. is among important legume crops. Agricultural producers prefer cultivars suitable for mechanized cultivation, with a determinate growth habit type. Plant architectonics depends on the functioning of the apical meristem, while the transition to the reproductive stage is controlled by a set of genes, including the TFL1 gene. Analyzing the genes responsible for the growth habit type is relevant for more efficient and rapid development of high-tech cultivars. Materials and methods. Using the Sanger DNA sequencing method, the primary structure of TFL1-like genes was studied in six cowpea accessions with different growth habit types and architectonics.Results. Promoter regions and coding parts of TFL1-like genes (VuTFL1.1, VuTFL1.2, VuATC, and VuBFT) were sequenced and analyzed. Information about the genes is available in the NCBI nucleotide sequence database. A comparative study showed that there were no exon differences between different genotypes. Rearrangements were found in the introns and the promoter region, but no relationship was traced between these rearrangements and the phenotype in terms of growth habit types or architectonics.Conclusion. The next step towards understanding the role of TFL1-like genes requires obtaining knockout lines based on these genes and studying their phenotype. Meanwhile, the results of this analysis call for a need to consider a wider range of cowpea genes potentially associated with the variability of stem growth habit types and plant architectonics.
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spelling doaj-art-26bd1ef04ea845c29bc053252ca7b2f62025-02-03T08:31:17ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822025-01-01185414314910.30901/2227-8834-2024-4-143-149844<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit typesE. A. Krylova0E. K. Khlestkina1N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic Resources; Sirius University of Science and Technology, Research Center of Genetics and Life SciencesBackground. Vigna unguiculata (L.) Walp. is among important legume crops. Agricultural producers prefer cultivars suitable for mechanized cultivation, with a determinate growth habit type. Plant architectonics depends on the functioning of the apical meristem, while the transition to the reproductive stage is controlled by a set of genes, including the TFL1 gene. Analyzing the genes responsible for the growth habit type is relevant for more efficient and rapid development of high-tech cultivars. Materials and methods. Using the Sanger DNA sequencing method, the primary structure of TFL1-like genes was studied in six cowpea accessions with different growth habit types and architectonics.Results. Promoter regions and coding parts of TFL1-like genes (VuTFL1.1, VuTFL1.2, VuATC, and VuBFT) were sequenced and analyzed. Information about the genes is available in the NCBI nucleotide sequence database. A comparative study showed that there were no exon differences between different genotypes. Rearrangements were found in the introns and the promoter region, but no relationship was traced between these rearrangements and the phenotype in terms of growth habit types or architectonics.Conclusion. The next step towards understanding the role of TFL1-like genes requires obtaining knockout lines based on these genes and studying their phenotype. Meanwhile, the results of this analysis call for a need to consider a wider range of cowpea genes potentially associated with the variability of stem growth habit types and plant architectonics.https://elpub.vir.nw.ru/jour/article/view/2119growth habit typecowpeatfl1-like genestfl1atcbft
spellingShingle E. A. Krylova
E. K. Khlestkina
<i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
Труды по прикладной ботанике, генетике и селекции
growth habit type
cowpea
tfl1-like genes
tfl1
atc
bft
title <i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
title_full <i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
title_fullStr <i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
title_full_unstemmed <i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
title_short <i>TFL1</i>-like genes in <i>Vigna unguiculata</i> (L.) Walp. with different growth habit types
title_sort i tfl1 i like genes in i vigna unguiculata i l walp with different growth habit types
topic growth habit type
cowpea
tfl1-like genes
tfl1
atc
bft
url https://elpub.vir.nw.ru/jour/article/view/2119
work_keys_str_mv AT eakrylova itfl1ilikegenesinivignaunguiculatailwalpwithdifferentgrowthhabittypes
AT ekkhlestkina itfl1ilikegenesinivignaunguiculatailwalpwithdifferentgrowthhabittypes