The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study

Abstract Background E-cigs(E-cigs) use is increasing worldwide. Recent studies suggest that E-cigs contain harmful elements that could lead to adverse oral health outcomes. Therefore, the aim of this study was to evaluate the impact of E-cigs smoking on periodontal health among current male smokers...

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Main Authors: Banna Alnufaiy, Hanadi Ghurmallah Alzahrani, Rhodanne A. Lambarte, Mohannad Abdullah Alghamdi, Abdulelah Saad Alali, Wael Farhan Alanazi, Mohammad Hamad Alkanhal, Abdulrahman Farhan Almukhalfi
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Language:English
Published: BMC 2025-05-01
Series:BMC Oral Health
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Online Access:https://doi.org/10.1186/s12903-025-06148-2
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author Banna Alnufaiy
Hanadi Ghurmallah Alzahrani
Rhodanne A. Lambarte
Mohannad Abdullah Alghamdi
Abdulelah Saad Alali
Wael Farhan Alanazi
Mohammad Hamad Alkanhal
Abdulrahman Farhan Almukhalfi
author_facet Banna Alnufaiy
Hanadi Ghurmallah Alzahrani
Rhodanne A. Lambarte
Mohannad Abdullah Alghamdi
Abdulelah Saad Alali
Wael Farhan Alanazi
Mohammad Hamad Alkanhal
Abdulrahman Farhan Almukhalfi
author_sort Banna Alnufaiy
collection DOAJ
description Abstract Background E-cigs(E-cigs) use is increasing worldwide. Recent studies suggest that E-cigs contain harmful elements that could lead to adverse oral health outcomes. Therefore, the aim of this study was to evaluate the impact of E-cigs smoking on periodontal health among current male smokers in Al-Kharj city in Saudi Arabia by assessing periodontal parameters and proinflammatory cytokine levels. Methods Fifty-three male individuals (25 E-smokers and 28 non-smokers) participated in the study. This study compared periodontal parameters, including the plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), clinical attachment loss (CAL), and marginal bone loss (MBL), as well as levels of unstimulated whole saliva (UWS), interleukin IL-1B, and IL-6, between E-smokers and non-smokers. Results The E-cigs users consumed approximately 432.6 ± 425.22 puffs on average daily, with a nicotine content of approximately 45.2 ± 11.23 mg on average. There was no statistically significant difference between the groups in terms of BOP and PI. A statistically significant difference was detected in PPD and CAL (p < 0.05) between the two groups, in which the PPD (4.10 ± 1.87) and CAL (2.72 ± 0.89) were greater in E-smokers. The mean MBL was also higher among E-smokers, which was statistically significant (p < 0.05). The mean cytokine IL-1B level was found to be (640.75 ± 138.78) among non-smokers and (889.05 ± 540.56) among E-smokers, and this difference was statistically significant (p < 0.05). However, while IL-1B had shown a significant difference between groups in the bivariate analysis (t-test), its association with E-cigs use became non-significant in the multivariate model (OR = 1.01, 95% CI: 1.00–1.02, p = 0.194). The mean IL-6 level among non-smokers was (19.49 ± 11.90) and among E-smokers, it was (17.07 ± 8.21). And, this difference was not statistically significant (p > 0.05). Conclusions This study revealed that E-cigs smoking had a negative effect on periodontal status (especially PPD, CAL and MBL). These results may contribute to the pathogenesis of periodontal diseases and tissue destruction. Efforts must be made to educate and create awareness among patients and the general community regarding the risks associated with E-cigs usage especially in young populations.
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publishDate 2025-05-01
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spelling doaj-art-26b07ed2e4ae4f4cbfab038e5e410a332025-08-20T03:08:43ZengBMCBMC Oral Health1472-68312025-05-0125111110.1186/s12903-025-06148-2The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional studyBanna Alnufaiy0Hanadi Ghurmallah Alzahrani1Rhodanne A. Lambarte2Mohannad Abdullah Alghamdi3Abdulelah Saad Alali4Wael Farhan Alanazi5Mohammad Hamad Alkanhal6Abdulrahman Farhan Almukhalfi7Department of Preventive Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz UniversityDepartment of Preventive Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz UniversityMolecular and Cell Biology Laboratory, Prince Naif bin AbdulAziz Health Research Center, College of Dentistry, King Saud University Medical City, King Saud UniversityBDS, Prince Sattam Bin Abdulaziz UniversityBDS, Prince Sattam Bin Abdulaziz UniversityBDS, Prince Sattam Bin Abdulaziz UniversityDental student, Prince Sattam Bin Abdulaziz UniversityDental student, Prince Sattam Bin Abdulaziz UniversityAbstract Background E-cigs(E-cigs) use is increasing worldwide. Recent studies suggest that E-cigs contain harmful elements that could lead to adverse oral health outcomes. Therefore, the aim of this study was to evaluate the impact of E-cigs smoking on periodontal health among current male smokers in Al-Kharj city in Saudi Arabia by assessing periodontal parameters and proinflammatory cytokine levels. Methods Fifty-three male individuals (25 E-smokers and 28 non-smokers) participated in the study. This study compared periodontal parameters, including the plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), clinical attachment loss (CAL), and marginal bone loss (MBL), as well as levels of unstimulated whole saliva (UWS), interleukin IL-1B, and IL-6, between E-smokers and non-smokers. Results The E-cigs users consumed approximately 432.6 ± 425.22 puffs on average daily, with a nicotine content of approximately 45.2 ± 11.23 mg on average. There was no statistically significant difference between the groups in terms of BOP and PI. A statistically significant difference was detected in PPD and CAL (p < 0.05) between the two groups, in which the PPD (4.10 ± 1.87) and CAL (2.72 ± 0.89) were greater in E-smokers. The mean MBL was also higher among E-smokers, which was statistically significant (p < 0.05). The mean cytokine IL-1B level was found to be (640.75 ± 138.78) among non-smokers and (889.05 ± 540.56) among E-smokers, and this difference was statistically significant (p < 0.05). However, while IL-1B had shown a significant difference between groups in the bivariate analysis (t-test), its association with E-cigs use became non-significant in the multivariate model (OR = 1.01, 95% CI: 1.00–1.02, p = 0.194). The mean IL-6 level among non-smokers was (19.49 ± 11.90) and among E-smokers, it was (17.07 ± 8.21). And, this difference was not statistically significant (p > 0.05). Conclusions This study revealed that E-cigs smoking had a negative effect on periodontal status (especially PPD, CAL and MBL). These results may contribute to the pathogenesis of periodontal diseases and tissue destruction. Efforts must be made to educate and create awareness among patients and the general community regarding the risks associated with E-cigs usage especially in young populations.https://doi.org/10.1186/s12903-025-06148-2Electronic cigarettesCytokinesPeriodontitisClinical attachment lossInterleukin
spellingShingle Banna Alnufaiy
Hanadi Ghurmallah Alzahrani
Rhodanne A. Lambarte
Mohannad Abdullah Alghamdi
Abdulelah Saad Alali
Wael Farhan Alanazi
Mohammad Hamad Alkanhal
Abdulrahman Farhan Almukhalfi
The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
BMC Oral Health
Electronic cigarettes
Cytokines
Periodontitis
Clinical attachment loss
Interleukin
title The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
title_full The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
title_fullStr The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
title_full_unstemmed The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
title_short The impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels: a cross-sectional study
title_sort impact of electronic cigarette smoking on periodontal status and proinflammatory cytokine levels a cross sectional study
topic Electronic cigarettes
Cytokines
Periodontitis
Clinical attachment loss
Interleukin
url https://doi.org/10.1186/s12903-025-06148-2
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