Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial

To evaluate the effects of probiotic (VSL#3) and omega-3 fatty acid on insulin sensitivity, blood lipids, and inflammation, we conducted a clinical trial in 60 overweight (BMI>25), healthy adults, aged 40–60 years. After initial screening the subjects were randomized into four groups with 15 per...

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Main Authors: Hemalatha Rajkumar, Naseha Mahmood, Manoj Kumar, Sudarshan Reddy Varikuti, Hanumanth Reddy Challa, Shiva Prakash Myakala
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2014/348959
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author Hemalatha Rajkumar
Naseha Mahmood
Manoj Kumar
Sudarshan Reddy Varikuti
Hanumanth Reddy Challa
Shiva Prakash Myakala
author_facet Hemalatha Rajkumar
Naseha Mahmood
Manoj Kumar
Sudarshan Reddy Varikuti
Hanumanth Reddy Challa
Shiva Prakash Myakala
author_sort Hemalatha Rajkumar
collection DOAJ
description To evaluate the effects of probiotic (VSL#3) and omega-3 fatty acid on insulin sensitivity, blood lipids, and inflammation, we conducted a clinical trial in 60 overweight (BMI>25), healthy adults, aged 40–60 years. After initial screening the subjects were randomized into four groups with 15 per group. The four groups received, respectively, placebo, omega-3 fatty acid, probiotic VSL#3, or both omega-3 and probiotic, for 6 weeks. Blood and fecal samples were collected at baseline and after 6 weeks. The probiotic (VSL#3) supplemented group had significant reduction in total cholesterol, triglyceride, LDL, and VLDL and had increased HDL (P<0.05) value. VSL#3 improved insulin sensitivity (P<0.01), decreased hsCRP, and favorably affected the composition of gut microbiota. Omega-3 had significant effect on insulin sensitivity and hsCRP but had no effect on gut microbiota. Addition of omega-3 fatty acid with VSL#3 had more pronounced effect on HDL, insulin sensitivity and hsCRP. Subjects with low HDL, insulin resistance, and high hsCRP had significantly lower total lactobacilli and bifidobacteria count and higher E. coli and bacteroides count.
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spelling doaj-art-26ae8a531d04428b84eb3c775a49296e2025-08-20T02:04:24ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/348959348959Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled TrialHemalatha Rajkumar0Naseha Mahmood1Manoj Kumar2Sudarshan Reddy Varikuti3Hanumanth Reddy Challa4Shiva Prakash Myakala5Department of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaDepartment of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaDepartment of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaDepartment of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaDepartment of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaDepartment of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, IndiaTo evaluate the effects of probiotic (VSL#3) and omega-3 fatty acid on insulin sensitivity, blood lipids, and inflammation, we conducted a clinical trial in 60 overweight (BMI>25), healthy adults, aged 40–60 years. After initial screening the subjects were randomized into four groups with 15 per group. The four groups received, respectively, placebo, omega-3 fatty acid, probiotic VSL#3, or both omega-3 and probiotic, for 6 weeks. Blood and fecal samples were collected at baseline and after 6 weeks. The probiotic (VSL#3) supplemented group had significant reduction in total cholesterol, triglyceride, LDL, and VLDL and had increased HDL (P<0.05) value. VSL#3 improved insulin sensitivity (P<0.01), decreased hsCRP, and favorably affected the composition of gut microbiota. Omega-3 had significant effect on insulin sensitivity and hsCRP but had no effect on gut microbiota. Addition of omega-3 fatty acid with VSL#3 had more pronounced effect on HDL, insulin sensitivity and hsCRP. Subjects with low HDL, insulin resistance, and high hsCRP had significantly lower total lactobacilli and bifidobacteria count and higher E. coli and bacteroides count.http://dx.doi.org/10.1155/2014/348959
spellingShingle Hemalatha Rajkumar
Naseha Mahmood
Manoj Kumar
Sudarshan Reddy Varikuti
Hanumanth Reddy Challa
Shiva Prakash Myakala
Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
Mediators of Inflammation
title Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
title_full Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
title_fullStr Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
title_full_unstemmed Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
title_short Effect of Probiotic (VSL#3) and Omega-3 on Lipid Profile, Insulin Sensitivity, Inflammatory Markers, and Gut Colonization in Overweight Adults: A Randomized, Controlled Trial
title_sort effect of probiotic vsl 3 and omega 3 on lipid profile insulin sensitivity inflammatory markers and gut colonization in overweight adults a randomized controlled trial
url http://dx.doi.org/10.1155/2014/348959
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