Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design
Fungi efficiently biosynthesize nanoparticles by utilizing their enzymatic and metabolic functions to reduce metal ions and improve colloidal stability. This study presents a sustainable method for producing silver nanoparticles through a cell-free extract from the fungus Alternaria sp. OP242500, em...
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Elsevier
2025-05-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715625002486 |
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| author | Saydeh Fatemeh Hoseini-Nilaki Morahem Ashengroph Musa Moetasam Zorab |
| author_facet | Saydeh Fatemeh Hoseini-Nilaki Morahem Ashengroph Musa Moetasam Zorab |
| author_sort | Saydeh Fatemeh Hoseini-Nilaki |
| collection | DOAJ |
| description | Fungi efficiently biosynthesize nanoparticles by utilizing their enzymatic and metabolic functions to reduce metal ions and improve colloidal stability. This study presents a sustainable method for producing silver nanoparticles through a cell-free extract from the fungus Alternaria sp. OP242500, emphasizing its value for green nanotechnology applications. Key synthesis parameters, including silver acetate concentration, pH, temperature, and incubation time, were optimized using the Box-Behnken design. Under optimal conditions (5.5 mM silver acetate, pH 7.8, 33.5 °C, and 96 h), the synthesized nanoparticles achieved an optical density of 2.11 at 430 nm, closely matching the predicted value of 2.15 with an accuracy of 98.14 %. Structural and stability analyses conducted through UV–visible spectroscopy, scanning electron microscopy, X-ray diffraction, zeta potential analysis, and Fourier-transform infrared spectroscopy confirmed the formation of uniformly spherical, well-crystallized silver nanoparticles with a face-centered cubic structure. The biosynthesized nanoparticles exhibited sizes ranging from 10.9 to 68.5 nm and displayed excellent colloidal stability, as evidenced by a zeta potential value of −20.8 mV. These findings demonstrate the efficiency of the fungal extract-based synthesis method and underscore its promising applications in medicine, nanotechnology, and environmental science. |
| format | Article |
| id | doaj-art-26a4ea5aa5f447a8909578a623809f67 |
| institution | OA Journals |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-26a4ea5aa5f447a8909578a623809f672025-08-20T02:09:05ZengElsevierResults in Chemistry2211-71562025-05-011510226510.1016/j.rechem.2025.102265Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken designSaydeh Fatemeh Hoseini-Nilaki0Morahem Ashengroph1Musa Moetasam Zorab2Department of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, IranDepartment of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran; Research Center for Nanotechnology, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran; Corresponding author at: Department of Biological Science, Faculty of Science, University of Kurdistan, P.O. Box 416, Sanandaj, Kurdistan, Iran.Department of Physics, College of science, University of Halabja, Kurdistan region, IraqFungi efficiently biosynthesize nanoparticles by utilizing their enzymatic and metabolic functions to reduce metal ions and improve colloidal stability. This study presents a sustainable method for producing silver nanoparticles through a cell-free extract from the fungus Alternaria sp. OP242500, emphasizing its value for green nanotechnology applications. Key synthesis parameters, including silver acetate concentration, pH, temperature, and incubation time, were optimized using the Box-Behnken design. Under optimal conditions (5.5 mM silver acetate, pH 7.8, 33.5 °C, and 96 h), the synthesized nanoparticles achieved an optical density of 2.11 at 430 nm, closely matching the predicted value of 2.15 with an accuracy of 98.14 %. Structural and stability analyses conducted through UV–visible spectroscopy, scanning electron microscopy, X-ray diffraction, zeta potential analysis, and Fourier-transform infrared spectroscopy confirmed the formation of uniformly spherical, well-crystallized silver nanoparticles with a face-centered cubic structure. The biosynthesized nanoparticles exhibited sizes ranging from 10.9 to 68.5 nm and displayed excellent colloidal stability, as evidenced by a zeta potential value of −20.8 mV. These findings demonstrate the efficiency of the fungal extract-based synthesis method and underscore its promising applications in medicine, nanotechnology, and environmental science.http://www.sciencedirect.com/science/article/pii/S2211715625002486Alternaria sp.Box-BenkenCell-free extractGreen synthesisSilver nanoparticles |
| spellingShingle | Saydeh Fatemeh Hoseini-Nilaki Morahem Ashengroph Musa Moetasam Zorab Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design Results in Chemistry Alternaria sp. Box-Benken Cell-free extract Green synthesis Silver nanoparticles |
| title | Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design |
| title_full | Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design |
| title_fullStr | Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design |
| title_full_unstemmed | Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design |
| title_short | Eco-friendly synthesis of silver nanoparticles using the fungus Alternaria sp. OP242500: Optimization through box-Behnken design |
| title_sort | eco friendly synthesis of silver nanoparticles using the fungus alternaria sp op242500 optimization through box behnken design |
| topic | Alternaria sp. Box-Benken Cell-free extract Green synthesis Silver nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S2211715625002486 |
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