OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis.
Vibrio cholerae is the causative agent of cholera, a dehydrating diarrheal disease. This Gram-negative pathogen is able to modulate its gene expression in order to combat stresses encountered in both aquatic and host environments, including stress posed by reactive oxygen species (ROS). In order to...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171201&type=printable |
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| author | Xiaoyun Xia Jessie Larios-Valencia Zhi Liu Fu Xiang Biao Kan Hui Wang Jun Zhu |
| author_facet | Xiaoyun Xia Jessie Larios-Valencia Zhi Liu Fu Xiang Biao Kan Hui Wang Jun Zhu |
| author_sort | Xiaoyun Xia |
| collection | DOAJ |
| description | Vibrio cholerae is the causative agent of cholera, a dehydrating diarrheal disease. This Gram-negative pathogen is able to modulate its gene expression in order to combat stresses encountered in both aquatic and host environments, including stress posed by reactive oxygen species (ROS). In order to further the understanding of V. cholerae's transcriptional response to ROS, we performed an RNA sequencing analysis to determine the transcriptional profile of V. cholerae when exposed to hydrogen hydroperoxide. Of 135 differentially expressed genes, VC0139 was amongst the genes with the largest induction. VC0139 encodes a protein homologous to the DPS (DNA-binding protein from starved cells) protein family, which are widely conserved and are implicated in ROS resistance in other bacteria. Using a promoter reporter assay, we show that during exponential growth, dps is induced by H2O2 in a manner dependent on the ROS-sensing transcriptional regulator, OxyR. Upon entry into stationary phase, the major stationary phase regulator RpoS is required to transcribe dps. Deletion of dps impaired V. cholerae resistance to both inorganic and organic hydroperoxides. Furthermore, we show that Dps is involved in resistance to multiple environmental stresses. Finally, we found that Dps is important for V. cholerae adult mouse colonization, but becomes dispensable in the presence of antioxidants. Taken together, our results suggest that Dps plays vital roles in both V. cholerae stress resistance and pathogenesis. |
| format | Article |
| id | doaj-art-26a2611c149f45bfb7da4c12cf4a42bd |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-26a2611c149f45bfb7da4c12cf4a42bd2025-08-20T03:04:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017120110.1371/journal.pone.0171201OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis.Xiaoyun XiaJessie Larios-ValenciaZhi LiuFu XiangBiao KanHui WangJun ZhuVibrio cholerae is the causative agent of cholera, a dehydrating diarrheal disease. This Gram-negative pathogen is able to modulate its gene expression in order to combat stresses encountered in both aquatic and host environments, including stress posed by reactive oxygen species (ROS). In order to further the understanding of V. cholerae's transcriptional response to ROS, we performed an RNA sequencing analysis to determine the transcriptional profile of V. cholerae when exposed to hydrogen hydroperoxide. Of 135 differentially expressed genes, VC0139 was amongst the genes with the largest induction. VC0139 encodes a protein homologous to the DPS (DNA-binding protein from starved cells) protein family, which are widely conserved and are implicated in ROS resistance in other bacteria. Using a promoter reporter assay, we show that during exponential growth, dps is induced by H2O2 in a manner dependent on the ROS-sensing transcriptional regulator, OxyR. Upon entry into stationary phase, the major stationary phase regulator RpoS is required to transcribe dps. Deletion of dps impaired V. cholerae resistance to both inorganic and organic hydroperoxides. Furthermore, we show that Dps is involved in resistance to multiple environmental stresses. Finally, we found that Dps is important for V. cholerae adult mouse colonization, but becomes dispensable in the presence of antioxidants. Taken together, our results suggest that Dps plays vital roles in both V. cholerae stress resistance and pathogenesis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171201&type=printable |
| spellingShingle | Xiaoyun Xia Jessie Larios-Valencia Zhi Liu Fu Xiang Biao Kan Hui Wang Jun Zhu OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. PLoS ONE |
| title | OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. |
| title_full | OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. |
| title_fullStr | OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. |
| title_full_unstemmed | OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. |
| title_short | OxyR-activated expression of Dps is important for Vibrio cholerae oxidative stress resistance and pathogenesis. |
| title_sort | oxyr activated expression of dps is important for vibrio cholerae oxidative stress resistance and pathogenesis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171201&type=printable |
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