The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection
Attachment and uptake into host cells are pivotal steps in the life cycle of the Chlamydiaceae, a family of obligate intracellular pathogens. Chlamydia trachomatis (Ctr) possesses a family of nine polymorphic membrane proteins (Pmps), which have been shown to be crucial for adhesion and internalizat...
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| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1519883/full |
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| author | Fabienne Kocher Johannes H. Hegemann |
| author_facet | Fabienne Kocher Johannes H. Hegemann |
| author_sort | Fabienne Kocher |
| collection | DOAJ |
| description | Attachment and uptake into host cells are pivotal steps in the life cycle of the Chlamydiaceae, a family of obligate intracellular pathogens. Chlamydia trachomatis (Ctr) possesses a family of nine polymorphic membrane proteins (Pmps), which have been shown to be crucial for adhesion and internalization. However, the host-cell molecules involved have so far remained unknown. Here, we show that a fragment of Ctr PmpD, which forms high-molecular-weight oligomers in solution and adheres to epithelial cells, also binds to secreted clusterin (sCLU), a chaperone-like protein that is secreted into the extracellular space by the host cell, and forms part of the chaperone- and receptor-mediated extracellular protein degradation (CRED) pathway. Using in vitro assays, we demonstrate that sCLU interacts directly with soluble rPmpD. In infection experiments, depletion of sCLU from the culture medium leads to a significant decrease in Ctr infection. Thus, sCLU is the first host-cell interaction partner identified for a Ctr Pmp and the first case in which sCLU has been shown to be a vital component for the establishment of a bacterial infection. |
| format | Article |
| id | doaj-art-269a6fd41062447493e23b12335c4b0e |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-269a6fd41062447493e23b12335c4b0e2025-01-27T06:40:35ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011410.3389/fcimb.2024.15198831519883The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infectionFabienne KocherJohannes H. HegemannAttachment and uptake into host cells are pivotal steps in the life cycle of the Chlamydiaceae, a family of obligate intracellular pathogens. Chlamydia trachomatis (Ctr) possesses a family of nine polymorphic membrane proteins (Pmps), which have been shown to be crucial for adhesion and internalization. However, the host-cell molecules involved have so far remained unknown. Here, we show that a fragment of Ctr PmpD, which forms high-molecular-weight oligomers in solution and adheres to epithelial cells, also binds to secreted clusterin (sCLU), a chaperone-like protein that is secreted into the extracellular space by the host cell, and forms part of the chaperone- and receptor-mediated extracellular protein degradation (CRED) pathway. Using in vitro assays, we demonstrate that sCLU interacts directly with soluble rPmpD. In infection experiments, depletion of sCLU from the culture medium leads to a significant decrease in Ctr infection. Thus, sCLU is the first host-cell interaction partner identified for a Ctr Pmp and the first case in which sCLU has been shown to be a vital component for the establishment of a bacterial infection.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1519883/fullPmpD adhesinsecreted human clusterininteractionChlamydia trachomatisinfection |
| spellingShingle | Fabienne Kocher Johannes H. Hegemann The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection Frontiers in Cellular and Infection Microbiology PmpD adhesin secreted human clusterin interaction Chlamydia trachomatis infection |
| title | The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection |
| title_full | The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection |
| title_fullStr | The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection |
| title_full_unstemmed | The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection |
| title_short | The secreted host-cell protein clusterin interacts with PmpD and promotes Chlamydia trachomatis infection |
| title_sort | secreted host cell protein clusterin interacts with pmpd and promotes chlamydia trachomatis infection |
| topic | PmpD adhesin secreted human clusterin interaction Chlamydia trachomatis infection |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1519883/full |
| work_keys_str_mv | AT fabiennekocher thesecretedhostcellproteinclusterininteractswithpmpdandpromoteschlamydiatrachomatisinfection AT johanneshhegemann thesecretedhostcellproteinclusterininteractswithpmpdandpromoteschlamydiatrachomatisinfection AT fabiennekocher secretedhostcellproteinclusterininteractswithpmpdandpromoteschlamydiatrachomatisinfection AT johanneshhegemann secretedhostcellproteinclusterininteractswithpmpdandpromoteschlamydiatrachomatisinfection |