Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses
Context: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymp...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2016-01-01
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| Series: | Indian Journal of Medical and Paediatric Oncology |
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| Online Access: | http://www.ijmpo.org/article.asp?issn=0971-5851;year=2016;volume=37;issue=3;spage=146;epage=151;aulast=Sinha |
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| author | Mahua Sinha Clementina Rama Rao C S Premalata Mohammed Shafiulla K C Lakshmaiah Linu Abraham Jacob Govind K Babu B K Viveka L Appaji Jayshree R Subramanyam |
| author_facet | Mahua Sinha Clementina Rama Rao C S Premalata Mohammed Shafiulla K C Lakshmaiah Linu Abraham Jacob Govind K Babu B K Viveka L Appaji Jayshree R Subramanyam |
| author_sort | Mahua Sinha |
| collection | DOAJ |
| description | Context: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymphomas (HLs). And recently, the lymphocyte-transforming role of hepatitis B virus (HBV) has been emphasized. Aims: The aim of this study was to elucidate the association of two potentially oncogenic, widely prevalent latent DNA viruses, EBV and HBV, in non-HL (NHL). Settings and Design: In this prospective study, we estimated plasma EBV and HBV DNA in NHL patients. Materials and Methods: Peripheral blood was obtained from newly diagnosed, treatment na ïve, histologically confirmed NHL patients. Plasma EBV DNA was quantified by real-time polymerase chain reaction (PCR) targeting Epstein–Barr Nucleic acid 1 while the plasma HBV DNA was detected using nested PCR targeting HBX gene. In a small subset of patients, follow-up plasma samples post-anticancer chemotherapy were available and retested for viral DNA. Results: Of the 110 NHL patients, ~79% were B-cell NHL and ~21% were T-cell NHL. Plasma EBV-DNA was detected in 10% NHLs with a higher EBV association in Burkitt lymphoma (33.3%) than other subtypes. Pretherapy HBV DNA was detected in 21% NHLs; most of them being diffuse large B-cell lymphoma (DLBCL). Moreover, 42% of DLBCL patients had HBV DNA in plasma. Since all patients were HBV surface antigen seronegative at diagnosis, baseline plasma HBV-DNAemia before chemotherapy was indicative of occult hepatitis B infection. Conclusions: Our findings indicate a significant association of HBV with newly diagnosed DLBCL. |
| format | Article |
| id | doaj-art-269849b7ea12452893ae38cb141b09b0 |
| institution | OA Journals |
| issn | 0971-5851 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Indian Journal of Medical and Paediatric Oncology |
| spelling | doaj-art-269849b7ea12452893ae38cb141b09b02025-08-20T01:51:27ZengThieme Medical and Scientific Publishers Pvt. Ltd.Indian Journal of Medical and Paediatric Oncology0971-58512016-01-0137314615110.4103/0971-5851.190353Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA virusesMahua SinhaClementina Rama RaoC S PremalataMohammed ShafiullaK C LakshmaiahLinu Abraham JacobGovind K BabuB K VivekaL AppajiJayshree R SubramanyamContext: There is a need to study potential infective etiologies in lymphomas. Lymphocyte-transforming viruses can directly infect lymphocytes, disrupt normal cell functions, and promote cell division. Epstein–Barr virus (EBV) is known to be associated with several lymphomas, especially Hodgkin lymphomas (HLs). And recently, the lymphocyte-transforming role of hepatitis B virus (HBV) has been emphasized. Aims: The aim of this study was to elucidate the association of two potentially oncogenic, widely prevalent latent DNA viruses, EBV and HBV, in non-HL (NHL). Settings and Design: In this prospective study, we estimated plasma EBV and HBV DNA in NHL patients. Materials and Methods: Peripheral blood was obtained from newly diagnosed, treatment na ïve, histologically confirmed NHL patients. Plasma EBV DNA was quantified by real-time polymerase chain reaction (PCR) targeting Epstein–Barr Nucleic acid 1 while the plasma HBV DNA was detected using nested PCR targeting HBX gene. In a small subset of patients, follow-up plasma samples post-anticancer chemotherapy were available and retested for viral DNA. Results: Of the 110 NHL patients, ~79% were B-cell NHL and ~21% were T-cell NHL. Plasma EBV-DNA was detected in 10% NHLs with a higher EBV association in Burkitt lymphoma (33.3%) than other subtypes. Pretherapy HBV DNA was detected in 21% NHLs; most of them being diffuse large B-cell lymphoma (DLBCL). Moreover, 42% of DLBCL patients had HBV DNA in plasma. Since all patients were HBV surface antigen seronegative at diagnosis, baseline plasma HBV-DNAemia before chemotherapy was indicative of occult hepatitis B infection. Conclusions: Our findings indicate a significant association of HBV with newly diagnosed DLBCL.http://www.ijmpo.org/article.asp?issn=0971-5851;year=2016;volume=37;issue=3;spage=146;epage=151;aulast=SinhaDiffuse large B-cell lymphomaEpstein–Barr virushepatitis B virusnon-Hodgkin lymphomaoccult hepatitis B virus infection |
| spellingShingle | Mahua Sinha Clementina Rama Rao C S Premalata Mohammed Shafiulla K C Lakshmaiah Linu Abraham Jacob Govind K Babu B K Viveka L Appaji Jayshree R Subramanyam Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses Indian Journal of Medical and Paediatric Oncology Diffuse large B-cell lymphoma Epstein–Barr virus hepatitis B virus non-Hodgkin lymphoma occult hepatitis B virus infection |
| title | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
| title_full | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
| title_fullStr | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
| title_full_unstemmed | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
| title_short | Plasma Epstein–Barr virus and Hepatitis B virus in non-Hodgkin lymphomas: Two lymphotropic, potentially oncogenic, latently occurring DNA viruses |
| title_sort | plasma epstein barr virus and hepatitis b virus in non hodgkin lymphomas two lymphotropic potentially oncogenic latently occurring dna viruses |
| topic | Diffuse large B-cell lymphoma Epstein–Barr virus hepatitis B virus non-Hodgkin lymphoma occult hepatitis B virus infection |
| url | http://www.ijmpo.org/article.asp?issn=0971-5851;year=2016;volume=37;issue=3;spage=146;epage=151;aulast=Sinha |
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