Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences
Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagid...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/275085 |
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| author | Yu-Hoon Kim Sang Eon Shin Chan Seon Ham Seong Yoon Kim Kwang Soo Ko Tae-Ho Jo Gi Hoon Son Seong Hwan Park Juck-Joon Hwang |
| author_facet | Yu-Hoon Kim Sang Eon Shin Chan Seon Ham Seong Yoon Kim Kwang Soo Ko Tae-Ho Jo Gi Hoon Son Seong Hwan Park Juck-Joon Hwang |
| author_sort | Yu-Hoon Kim |
| collection | DOAJ |
| description | Identification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola. |
| format | Article |
| id | doaj-art-2696331fe7394492905fa7d4126fcab4 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2696331fe7394492905fa7d4126fcab42025-02-03T01:30:01ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/275085275085Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide SequencesYu-Hoon Kim0Sang Eon Shin1Chan Seon Ham2Seong Yoon Kim3Kwang Soo Ko4Tae-Ho Jo5Gi Hoon Son6Seong Hwan Park7Juck-Joon Hwang8Department of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Science Education, Chinju National University of Education, 3 Jinyangho-ro 369 gil, Jinju 660-756, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaDepartment of Legal Medicine, Korea University College of Medicine, Inchonro 73, Seongbukgu, Seoul 136-705, Republic of KoreaIdentification of insect species is an important task in forensic entomology. For more convenient species identification, the nucleotide sequences of cytochrome c oxidase subunit I (COI) gene have been widely utilized. We analyzed full-length COI nucleotide sequences of 10 Muscidae and 6 Sarcophagidae fly species collected in Korea. After DNA extraction from collected flies, PCR amplification and automatic sequencing of the whole COI sequence were performed. Obtained sequences were analyzed for a phylogenetic tree and a distance matrix. Our data showed very low intraspecific sequence distances and species-level monophylies. However, sequence comparison with previously reported sequences revealed a few inconsistencies or paraphylies requiring further investigation. To the best of our knowledge, this study is the first report of COI nucleotide sequences from Hydrotaea occulta, Muscina angustifrons, Muscina pascuorum, Ophyra leucostoma, Sarcophaga haemorrhoidalis, Sarcophaga harpax, and Phaonia aureola.http://dx.doi.org/10.1155/2014/275085 |
| spellingShingle | Yu-Hoon Kim Sang Eon Shin Chan Seon Ham Seong Yoon Kim Kwang Soo Ko Tae-Ho Jo Gi Hoon Son Seong Hwan Park Juck-Joon Hwang Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences The Scientific World Journal |
| title | Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences |
| title_full | Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences |
| title_fullStr | Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences |
| title_full_unstemmed | Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences |
| title_short | Molecular Identification of Necrophagous Muscidae and Sarcophagidae Fly Species Collected in Korea by Mitochondrial Cytochrome c Oxidase Subunit I Nucleotide Sequences |
| title_sort | molecular identification of necrophagous muscidae and sarcophagidae fly species collected in korea by mitochondrial cytochrome c oxidase subunit i nucleotide sequences |
| url | http://dx.doi.org/10.1155/2014/275085 |
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