Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves
Summary: Leaf color patterns in nature, shaped by genetic and environmental factors, can be analyzed using hyperspectral reflectance imaging. This protocol details step-by-step procedures for hyperspectral image acquisition, correction of uneven lighting, and spectral component analysis to reveal di...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002606 |
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| author | Shalini Krishnamoorthi Daisuke Urano |
| author_facet | Shalini Krishnamoorthi Daisuke Urano |
| author_sort | Shalini Krishnamoorthi |
| collection | DOAJ |
| description | Summary: Leaf color patterns in nature, shaped by genetic and environmental factors, can be analyzed using hyperspectral reflectance imaging. This protocol details step-by-step procedures for hyperspectral image acquisition, correction of uneven lighting, and spectral component analysis to reveal distinct and sometimes previously undetectable features on leaves. We outline how to identify key spectral components and project hyperspectral cubes onto them to highlight specific spectral traits.For complete details of this protocol, please refer to Krishnamoorthi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-2678039f4f8244c4a2d7d4c3f9bc1eff |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-2678039f4f8244c4a2d7d4c3f9bc1eff2025-08-20T02:37:38ZengElsevierSTAR Protocols2666-16672025-06-016210385410.1016/j.xpro.2025.103854Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leavesShalini Krishnamoorthi0Daisuke Urano1Temasek Life Sciences Laboratory, Singapore 117604, SingaporeTemasek Life Sciences Laboratory, Singapore 117604, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore; Corresponding authorSummary: Leaf color patterns in nature, shaped by genetic and environmental factors, can be analyzed using hyperspectral reflectance imaging. This protocol details step-by-step procedures for hyperspectral image acquisition, correction of uneven lighting, and spectral component analysis to reveal distinct and sometimes previously undetectable features on leaves. We outline how to identify key spectral components and project hyperspectral cubes onto them to highlight specific spectral traits.For complete details of this protocol, please refer to Krishnamoorthi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002606High Throughput ScreeningMicroscopyPlant sciencesComputer sciences |
| spellingShingle | Shalini Krishnamoorthi Daisuke Urano Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves STAR Protocols High Throughput Screening Microscopy Plant sciences Computer sciences |
| title | Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| title_full | Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| title_fullStr | Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| title_full_unstemmed | Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| title_short | Hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| title_sort | hyperspectral reflectance imaging and spectral component analysis techniques to reveal distinct color patterns on plant leaves |
| topic | High Throughput Screening Microscopy Plant sciences Computer sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002606 |
| work_keys_str_mv | AT shalinikrishnamoorthi hyperspectralreflectanceimagingandspectralcomponentanalysistechniquestorevealdistinctcolorpatternsonplantleaves AT daisukeurano hyperspectralreflectanceimagingandspectralcomponentanalysistechniquestorevealdistinctcolorpatternsonplantleaves |