Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates
Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria. Methodology: a col...
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The Journal of Infection in Developing Countries
2021-01-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12482 |
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| author | Nabila Benamrouche Badia Guettou Fatma Zohra Henniche Farida Assaous Houcine Laouar Hanifa Ziane Fazia Djennane Djamal Tiouit Chafia Bentchouala Ferroudja Yamouni Kheira Rahal Hassiba Tali Maamar |
| author_facet | Nabila Benamrouche Badia Guettou Fatma Zohra Henniche Farida Assaous Houcine Laouar Hanifa Ziane Fazia Djennane Djamal Tiouit Chafia Bentchouala Ferroudja Yamouni Kheira Rahal Hassiba Tali Maamar |
| author_sort | Nabila Benamrouche |
| collection | DOAJ |
| description |
Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria.
Methodology: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST).
Results: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17.
Conclusions: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures.
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| format | Article |
| id | doaj-art-2658d02832b841c3a5cd609648bf00da |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-2658d02832b841c3a5cd609648bf00da2025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-01-01150110.3855/jidc.12482Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolatesNabila Benamrouche0Badia Guettou1Fatma Zohra Henniche2Farida Assaous3Houcine Laouar4Hanifa Ziane5Fazia Djennane6Djamal Tiouit7Chafia Bentchouala8Ferroudja Yamouni9Kheira Rahal10Hassiba Tali Maamar11Medical Bacteriology Laboratory, Institut Pasteur, Algiers, AlgeriaMedical Bacteriology Laboratory, Institut Pasteur, Algiers, AlgeriaMicrobiology Laboratory, Mohamed Seghir Nekkache Central Hospital of Army, Algiers, AlgeriaMedical Bacteriology Laboratory, Institut Pasteur, Algiers, AlgeriaDepartment of Microbiology, University Hospital of Constantine, Constantine, AlgeriaDepartment of Microbiology, Mustapha University Hospital, Algiers, AlgeriaDepartment of Microbiology, Mustapha University Hospital, Algiers, AlgeriaMicrobiology Laboratory, Mohamed Seghir Nekkache Central Hospital of Army, Algiers, AlgeriaDepartment of Microbiology, University Hospital of Constantine, Constantine, AlgeriaCentral Laboratory, University Regional Military Hospital of Oran, Oran, AlgeriaMedical Bacteriology Laboratory, Institut Pasteur, Algiers, AlgeriaMedical Bacteriology Laboratory, Institut Pasteur, Algiers, Algeria Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria. Methodology: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST). Results: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17. Conclusions: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures. https://jidc.org/index.php/journal/article/view/12482E. faeciumantimicrobial susceptibilityvanAMLSTAlgeriavancomycin resistant |
| spellingShingle | Nabila Benamrouche Badia Guettou Fatma Zohra Henniche Farida Assaous Houcine Laouar Hanifa Ziane Fazia Djennane Djamal Tiouit Chafia Bentchouala Ferroudja Yamouni Kheira Rahal Hassiba Tali Maamar Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates Journal of Infection in Developing Countries E. faecium antimicrobial susceptibility vanA MLST Algeria vancomycin resistant |
| title | Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates |
| title_full | Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates |
| title_fullStr | Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates |
| title_full_unstemmed | Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates |
| title_short | Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates |
| title_sort | vancomycin resistant enterococcus faecium in algeria phenotypic and genotypic characterization of clinical isolates |
| topic | E. faecium antimicrobial susceptibility vanA MLST Algeria vancomycin resistant |
| url | https://jidc.org/index.php/journal/article/view/12482 |
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