Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling
<i>Euphorbia jolkinii</i> dominates the subalpine meadows in Shangri-La (Southwest China) owing to its potent allelopathic effects. However, the effects underlying its allelopathy require further characterization at the physiological and molecular levels. In this study, the physiological...
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2025-01-01
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| author | Xue Xiao Zuyan Ma Kai Zhou Qiongmei Niu Qin Luo Xin Yang Xiaohui Chu Guilian Shan |
| author_facet | Xue Xiao Zuyan Ma Kai Zhou Qiongmei Niu Qin Luo Xin Yang Xiaohui Chu Guilian Shan |
| author_sort | Xue Xiao |
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| description | <i>Euphorbia jolkinii</i> dominates the subalpine meadows in Shangri-La (Southwest China) owing to its potent allelopathic effects. However, the effects underlying its allelopathy require further characterization at the physiological and molecular levels. In this study, the physiological, biochemical, and metabolic mechanisms underlying <i>E. jolkinii</i> allelopathy were investigated using <i>Arundinella hookeri</i> as a receptor plant. The treatment of <i>A. hookeri</i> seedlings with <i>E. jolkinii</i> aqueous extract (EJAE) disrupted their growth by inhibiting photosynthesis, disrupting oxidation systems, and increasing soluble sugar accumulation and chlorophyll synthesis. Collectively, this causes severe impairment accompanied by abnormal photosynthesis and reduced biomass accumulation. Moreover, EJAE treatment suppressed gibberellin, indoleacetic acid, zeatin, salicylic acid, and jasmonic acid levels while promoting abscisic acid accumulation. Further metabolomic analyses identified numerous differentially abundant metabolites primarily enriched in the α-linolenic, phenylpropanoid, and flavonoid biosynthesis pathways in EJAE-treated <i>A. hookeri</i> seedlings. This study demonstrated that <i>E. jolkinii</i> exhibits potent and comprehensive allelopathic effects on receptor plants, including a significant disruption of endogenous hormone synthesis, the inhibition of photosynthesis, an impairment of membrane and oxidation systems, and changes in crucial metabolic processes associated with α-linolenic, phenylpropanoid, and flavonoid biosynthesis. Thus, our study provides a solid theoretical foundation for understanding the regulatory mechanisms underlying <i>E. jolkinii</i> allelopathy. |
| format | Article |
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| institution | Kabale University |
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| language | English |
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| series | Plants |
| spelling | doaj-art-26562fa50cbc4c0db0066dc46395dc4e2025-01-10T13:19:49ZengMDPI AGPlants2223-77472025-01-0114112310.3390/plants14010123Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics ProfilingXue Xiao0Zuyan Ma1Kai Zhou2Qiongmei Niu3Qin Luo4Xin Yang5Xiaohui Chu6Guilian Shan7Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China<i>Euphorbia jolkinii</i> dominates the subalpine meadows in Shangri-La (Southwest China) owing to its potent allelopathic effects. However, the effects underlying its allelopathy require further characterization at the physiological and molecular levels. In this study, the physiological, biochemical, and metabolic mechanisms underlying <i>E. jolkinii</i> allelopathy were investigated using <i>Arundinella hookeri</i> as a receptor plant. The treatment of <i>A. hookeri</i> seedlings with <i>E. jolkinii</i> aqueous extract (EJAE) disrupted their growth by inhibiting photosynthesis, disrupting oxidation systems, and increasing soluble sugar accumulation and chlorophyll synthesis. Collectively, this causes severe impairment accompanied by abnormal photosynthesis and reduced biomass accumulation. Moreover, EJAE treatment suppressed gibberellin, indoleacetic acid, zeatin, salicylic acid, and jasmonic acid levels while promoting abscisic acid accumulation. Further metabolomic analyses identified numerous differentially abundant metabolites primarily enriched in the α-linolenic, phenylpropanoid, and flavonoid biosynthesis pathways in EJAE-treated <i>A. hookeri</i> seedlings. This study demonstrated that <i>E. jolkinii</i> exhibits potent and comprehensive allelopathic effects on receptor plants, including a significant disruption of endogenous hormone synthesis, the inhibition of photosynthesis, an impairment of membrane and oxidation systems, and changes in crucial metabolic processes associated with α-linolenic, phenylpropanoid, and flavonoid biosynthesis. Thus, our study provides a solid theoretical foundation for understanding the regulatory mechanisms underlying <i>E. jolkinii</i> allelopathy.https://www.mdpi.com/2223-7747/14/1/123<i>Euphorbia jolkinii</i>allelopathyphotosynthesisphytohormonemetabolomicsecondary metabolism |
| spellingShingle | Xue Xiao Zuyan Ma Kai Zhou Qiongmei Niu Qin Luo Xin Yang Xiaohui Chu Guilian Shan Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling Plants <i>Euphorbia jolkinii</i> allelopathy photosynthesis phytohormone metabolomic secondary metabolism |
| title | Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling |
| title_full | Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling |
| title_fullStr | Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling |
| title_full_unstemmed | Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling |
| title_short | Elucidating the Underlying Allelopathy Effects of <i>Euphorbia jolkinii</i> on <i>Arundinella hookeri</i> Using Metabolomics Profiling |
| title_sort | elucidating the underlying allelopathy effects of i euphorbia jolkinii i on i arundinella hookeri i using metabolomics profiling |
| topic | <i>Euphorbia jolkinii</i> allelopathy photosynthesis phytohormone metabolomic secondary metabolism |
| url | https://www.mdpi.com/2223-7747/14/1/123 |
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