Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay
Peanut allergy is considered to be a major health issue with global effects. To date, no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens. The accurate and effective detection methods for the surveillance of allergens in food are sti...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001951 |
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| author | Yaozhong Hu Chuan Zhang Jing Lin Yi Wang Sihao Wu Ying Sun Bowei Zhang Huan Lv Xuemeng Ji Yang Lu Shuo Wang |
| author_facet | Yaozhong Hu Chuan Zhang Jing Lin Yi Wang Sihao Wu Ying Sun Bowei Zhang Huan Lv Xuemeng Ji Yang Lu Shuo Wang |
| author_sort | Yaozhong Hu |
| collection | DOAJ |
| description | Peanut allergy is considered to be a major health issue with global effects. To date, no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens. The accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic diseases. In this study, nanobodies (Nbs) derived from the Heavy-Chain only Antibodies (HCAbs) were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen contamination. The target antigen of the selected Nb was identified as peanut allergen Ara h 3, and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 family. The applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified. A sandwich ELISA against peanut allergen was developed, which reached a linear range of 0.2－10.6 μg/mL, and a limit of detection of 53.13 ng/mL. |
| format | Article |
| id | doaj-art-264d043b5145425a957416838246109a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-264d043b5145425a957416838246109a2025-02-03T02:34:36ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123745754Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assayYaozhong Hu0Chuan Zhang1Jing Lin2Yi Wang3Sihao Wu4Ying Sun5Bowei Zhang6Huan Lv7Xuemeng Ji8Yang Lu9Shuo Wang10Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, ChinaCollege of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China; Corresponding author at: Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.Peanut allergy is considered to be a major health issue with global effects. To date, no effective curative approach has been applied for the therapy of the anaphylaxis resulting from the peanut allergens. The accurate and effective detection methods for the surveillance of allergens in food are still the primary strategies to avoid allergic diseases. In this study, nanobodies (Nbs) derived from the Heavy-Chain only Antibodies (HCAbs) were selected against the general peanut protein extract through the unbiased strategy to facilitate the development of the sandwich ELISA for the detection and surveillance of peanut allergen contamination. The target antigen of the selected Nb was identified as peanut allergen Ara h 3, and a cross-reaction was observed with the member of Gly 1 from the Ara h 3 family. The applicability of the self-paired Nb P43 on the establishment of the immuno-assay was verified. A sandwich ELISA against peanut allergen was developed, which reached a linear range of 0.2－10.6 μg/mL, and a limit of detection of 53.13 ng/mL.http://www.sciencedirect.com/science/article/pii/S2213453022001951Peanut allergenAra h 3NanobodySandwich ELISAGly 1 |
| spellingShingle | Yaozhong Hu Chuan Zhang Jing Lin Yi Wang Sihao Wu Ying Sun Bowei Zhang Huan Lv Xuemeng Ji Yang Lu Shuo Wang Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay Food Science and Human Wellness Peanut allergen Ara h 3 Nanobody Sandwich ELISA Gly 1 |
| title | Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay |
| title_full | Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay |
| title_fullStr | Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay |
| title_full_unstemmed | Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay |
| title_short | Selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno-assay |
| title_sort | selection of specific nanobodies against peanut allergen through unbiased immunization strategy and the developed immuno assay |
| topic | Peanut allergen Ara h 3 Nanobody Sandwich ELISA Gly 1 |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001951 |
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