Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury.
<h4>Background</h4>Shenfu injection (SFI), derived from a traditional Chinese medicine (TCM) prescription, is an effective drug for the treatment of sepsis-induced myocardial injury (SIMI) with good efficacy, but its exact therapeutic mechanism remains unclear.<h4>Methods</h4>...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0317738 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850043070834278400 |
|---|---|
| author | Hua-Jing Yuan Guo-Han Xiang Yang Liu Yan Li Wen-Li Liu Jiu-Xiang Wei Yi-Tao Xue Hao Hao |
| author_facet | Hua-Jing Yuan Guo-Han Xiang Yang Liu Yan Li Wen-Li Liu Jiu-Xiang Wei Yi-Tao Xue Hao Hao |
| author_sort | Hua-Jing Yuan |
| collection | DOAJ |
| description | <h4>Background</h4>Shenfu injection (SFI), derived from a traditional Chinese medicine (TCM) prescription, is an effective drug for the treatment of sepsis-induced myocardial injury (SIMI) with good efficacy, but its exact therapeutic mechanism remains unclear.<h4>Methods</h4>SwissTargetPrediction and GeneCards database were used to obtain relevant targets for SFI and SIMI. STRING 11.5 and MCODE were used to analyse potential therapeutic targets for SFI. DAVID 6.8 database was used to perform enrichment analysis. In addition, the SIMI model was constructed by cecal ligation and puncture (CLP) on Sprague Dawley rats and the related protein expression levels were verified by AutoDock Vina 1.1.2 and experiment.<h4>Results</h4>SFI has a total of 10 main active compounds and treats SIMI through 52 potential targets, among which LGALS3, STAT3, FGF1, and AKT1 were the core targets for treatment. Based on enrichment analysis, STAT3, FGF1, and AKT1 in the core targets were experimentally validated. The experimental results showed that SFI effectively ameliorated the inflammatory response and myocardial injury and inhibited apoptosis in SIMI. And SFI improved SIMI by decreasing caspase-9, STAT3 and phospho-AKT1 (p-AKT1) expression, and enhancing FGF1 expressions.<h4>Conclusions</h4>This study showed that SFI effectively reduced the expression of caspase-9, STAT3 and p-AKT1, enhanced the expression of FGF1, reduced the inflammatory response, inhibited apoptosis and attenuated cardiac injury to SIMI. |
| format | Article |
| id | doaj-art-264b685784d24a8a8491441ecd4d71cf |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-264b685784d24a8a8491441ecd4d71cf2025-08-20T02:55:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031773810.1371/journal.pone.0317738Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury.Hua-Jing YuanGuo-Han XiangYang LiuYan LiWen-Li LiuJiu-Xiang WeiYi-Tao XueHao Hao<h4>Background</h4>Shenfu injection (SFI), derived from a traditional Chinese medicine (TCM) prescription, is an effective drug for the treatment of sepsis-induced myocardial injury (SIMI) with good efficacy, but its exact therapeutic mechanism remains unclear.<h4>Methods</h4>SwissTargetPrediction and GeneCards database were used to obtain relevant targets for SFI and SIMI. STRING 11.5 and MCODE were used to analyse potential therapeutic targets for SFI. DAVID 6.8 database was used to perform enrichment analysis. In addition, the SIMI model was constructed by cecal ligation and puncture (CLP) on Sprague Dawley rats and the related protein expression levels were verified by AutoDock Vina 1.1.2 and experiment.<h4>Results</h4>SFI has a total of 10 main active compounds and treats SIMI through 52 potential targets, among which LGALS3, STAT3, FGF1, and AKT1 were the core targets for treatment. Based on enrichment analysis, STAT3, FGF1, and AKT1 in the core targets were experimentally validated. The experimental results showed that SFI effectively ameliorated the inflammatory response and myocardial injury and inhibited apoptosis in SIMI. And SFI improved SIMI by decreasing caspase-9, STAT3 and phospho-AKT1 (p-AKT1) expression, and enhancing FGF1 expressions.<h4>Conclusions</h4>This study showed that SFI effectively reduced the expression of caspase-9, STAT3 and p-AKT1, enhanced the expression of FGF1, reduced the inflammatory response, inhibited apoptosis and attenuated cardiac injury to SIMI.https://doi.org/10.1371/journal.pone.0317738 |
| spellingShingle | Hua-Jing Yuan Guo-Han Xiang Yang Liu Yan Li Wen-Li Liu Jiu-Xiang Wei Yi-Tao Xue Hao Hao Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. PLoS ONE |
| title | Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. |
| title_full | Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. |
| title_fullStr | Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. |
| title_full_unstemmed | Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. |
| title_short | Exploration and verification of the therapeutic mechanism of shenfu injection in sepsis-induced myocardial injury. |
| title_sort | exploration and verification of the therapeutic mechanism of shenfu injection in sepsis induced myocardial injury |
| url | https://doi.org/10.1371/journal.pone.0317738 |
| work_keys_str_mv | AT huajingyuan explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT guohanxiang explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT yangliu explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT yanli explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT wenliliu explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT jiuxiangwei explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT yitaoxue explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury AT haohao explorationandverificationofthetherapeuticmechanismofshenfuinjectioninsepsisinducedmyocardialinjury |