Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’
Dormancy release is an important process for improving the quality of cut-flower lily production and promoting the factory production of lily bulbs. However, the regulatory mechanisms of microRNAs (miRNAs) and their target genes during the dormancy release of lily remain elusive. Anatomy, transcript...
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2024-12-01
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| author | Xiaoping Xu Chenglong Yang Yiping Zheng Wenjie Guo Zhongxiong Lai Shaozhong Fang |
| author_facet | Xiaoping Xu Chenglong Yang Yiping Zheng Wenjie Guo Zhongxiong Lai Shaozhong Fang |
| author_sort | Xiaoping Xu |
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| description | Dormancy release is an important process for improving the quality of cut-flower lily production and promoting the factory production of lily bulbs. However, the regulatory mechanisms of microRNAs (miRNAs) and their target genes during the dormancy release of lily remain elusive. Anatomy, transcriptomic, molecular biology, and transient transformation techniques involving subcellular localization were applied in our study. There were significant results showing that 0.1 mM riboflavin promoted dormancy release and floral bud differentiation and influenced the flowering time of the <i>Lilium</i> ‘Siberia’. Moreover, some differentially expressed miRNAs and their targets (miR395-y: <i>LoAPS1</i>, miR529-z: <i>LoSPL14</i>, miR396-y: <i>LoCFDP1</i>, miR1863-z: <i>LoFBA3</i>, miR399-y: <i>LoDIT1</i>, and miR11525-z: <i>Lopgm</i>) were identified and predicted. Exogenous riboflavin may activate primary metabolic processes and promote dormancy release in <i>Lilium</i> ‘Siberia’ bulbs. Furthermore, riboflavin upregulated genes related to the riboflavin pathway, H3K4me3 methylation, dormancy control, and the flowering pathway and downregulated dormancy maintenance genes. Moreover, riboflavin promoted endogenous riboflavin and acetyl-CoA accumulation. <i>LoPurple acid phosphatase17</i> (<i>LoPAP17</i>), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of <i>Acorus calamus</i>, <i>Asparagus officinalis</i>, and <i>Musa acuminata</i>. The LoPAP17 protein was subcellularly located in the nucleus. Our study revealed that miRNAs and their target genes might regulate the primary metabolic pathway, promote the accumulation of endogenous riboflavin and acetyl-CoA, and affect protein acetylation during the riboflavin-promoted release of dormancy and flower bud differentiation in the <i>Lilium</i> Oriental hybrid ‘Siberia’. |
| format | Article |
| id | doaj-art-2626b7c928a44cfa89e27c93e72988b8 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Horticulturae |
| spelling | doaj-art-2626b7c928a44cfa89e27c93e72988b82025-01-24T13:34:29ZengMDPI AGHorticulturae2311-75242024-12-011111710.3390/horticulturae11010017Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’Xiaoping Xu0Chenglong Yang1Yiping Zheng2Wenjie Guo3Zhongxiong Lai4Shaozhong Fang5Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350001, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350001, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350001, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350001, ChinaInstitute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou 350001, ChinaDormancy release is an important process for improving the quality of cut-flower lily production and promoting the factory production of lily bulbs. However, the regulatory mechanisms of microRNAs (miRNAs) and their target genes during the dormancy release of lily remain elusive. Anatomy, transcriptomic, molecular biology, and transient transformation techniques involving subcellular localization were applied in our study. There were significant results showing that 0.1 mM riboflavin promoted dormancy release and floral bud differentiation and influenced the flowering time of the <i>Lilium</i> ‘Siberia’. Moreover, some differentially expressed miRNAs and their targets (miR395-y: <i>LoAPS1</i>, miR529-z: <i>LoSPL14</i>, miR396-y: <i>LoCFDP1</i>, miR1863-z: <i>LoFBA3</i>, miR399-y: <i>LoDIT1</i>, and miR11525-z: <i>Lopgm</i>) were identified and predicted. Exogenous riboflavin may activate primary metabolic processes and promote dormancy release in <i>Lilium</i> ‘Siberia’ bulbs. Furthermore, riboflavin upregulated genes related to the riboflavin pathway, H3K4me3 methylation, dormancy control, and the flowering pathway and downregulated dormancy maintenance genes. Moreover, riboflavin promoted endogenous riboflavin and acetyl-CoA accumulation. <i>LoPurple acid phosphatase17</i> (<i>LoPAP17</i>), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of <i>Acorus calamus</i>, <i>Asparagus officinalis</i>, and <i>Musa acuminata</i>. The LoPAP17 protein was subcellularly located in the nucleus. Our study revealed that miRNAs and their target genes might regulate the primary metabolic pathway, promote the accumulation of endogenous riboflavin and acetyl-CoA, and affect protein acetylation during the riboflavin-promoted release of dormancy and flower bud differentiation in the <i>Lilium</i> Oriental hybrid ‘Siberia’.https://www.mdpi.com/2311-7524/11/1/17riboflavin<i>Lilium</i> Oriental hybrid ‘Siberia’miRNAtranscriptomicsdormancy release |
| spellingShingle | Xiaoping Xu Chenglong Yang Yiping Zheng Wenjie Guo Zhongxiong Lai Shaozhong Fang Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ Horticulturae riboflavin <i>Lilium</i> Oriental hybrid ‘Siberia’ miRNA transcriptomics dormancy release |
| title | Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ |
| title_full | Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ |
| title_fullStr | Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ |
| title_full_unstemmed | Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ |
| title_short | Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in <i>Lilium</i> ‘Siberia’ |
| title_sort | transcriptome wide identification of mirnas and their targets during riboflavin promoted dormancy release in i lilium i siberia |
| topic | riboflavin <i>Lilium</i> Oriental hybrid ‘Siberia’ miRNA transcriptomics dormancy release |
| url | https://www.mdpi.com/2311-7524/11/1/17 |
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