Purification of Peroxisomes in a Self-Generated Gradient
In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but n...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.836 |
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| _version_ | 1849306684769435648 |
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| author | John Graham |
| author_facet | John Graham |
| author_sort | John Graham |
| collection | DOAJ |
| description | In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in PercollⓇ there is no contamination from endoplasmic reticulum. |
| format | Article |
| id | doaj-art-2602da8a35404cd4929455f69eebacd4 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2602da8a35404cd4929455f69eebacd42025-08-20T03:55:00ZengWileyThe Scientific World Journal1537-744X2002-01-0121532153510.1100/tsw.2002.836Purification of Peroxisomes in a Self-Generated GradientJohn Graham0School of Biomolecular Sciences, Liverpool John Moores University, UKIn iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in PercollⓇ there is no contamination from endoplasmic reticulum.http://dx.doi.org/10.1100/tsw.2002.836 |
| spellingShingle | John Graham Purification of Peroxisomes in a Self-Generated Gradient The Scientific World Journal |
| title | Purification of Peroxisomes in a Self-Generated Gradient |
| title_full | Purification of Peroxisomes in a Self-Generated Gradient |
| title_fullStr | Purification of Peroxisomes in a Self-Generated Gradient |
| title_full_unstemmed | Purification of Peroxisomes in a Self-Generated Gradient |
| title_short | Purification of Peroxisomes in a Self-Generated Gradient |
| title_sort | purification of peroxisomes in a self generated gradient |
| url | http://dx.doi.org/10.1100/tsw.2002.836 |
| work_keys_str_mv | AT johngraham purificationofperoxisomesinaselfgeneratedgradient |