A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms
A simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of rabeprazole in bulk drug samples and formulations. Rabeprazole was analyzed by using reverse phase LC-GC column (Inertsil ODS, 4.6 mm x 25 cm, 5 micr...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/412194 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849306682089275392 |
|---|---|
| author | Uma Mahesh Karra Sanjeeva Yarkala |
| author_facet | Uma Mahesh Karra Sanjeeva Yarkala |
| author_sort | Uma Mahesh Karra |
| collection | DOAJ |
| description | A simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of rabeprazole in bulk drug samples and formulations. Rabeprazole was analyzed by using reverse phase LC-GC column (Inertsil ODS, 4.6 mm x 25 cm, 5 microns), with mobile phase consisting of methanol: water (78:22 v/v). The flow rate was set 1.0 mL/min and analysis was performed at wavelength 288 nm using Photo Diode Array (PDA) detector at ambient temperature. The method was validated and stability studies were conducted under different conditions. The retention time for rabeprazole was around 4.12 minutes. The calibration curves were linear (r≥0.9998) over a concentration range from 20.0 to 80.0 μg/mL. Limit of detection (LOD) and Limit of quantitation (LOQ) were 8 ng/mL and 24 ng/mL respectively. The developed method was successfully applied to estimate the amount of rabeprazole in tablet formulations. |
| format | Article |
| id | doaj-art-25fe5e2bd4334ca494ab95a984a93051 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-25fe5e2bd4334ca494ab95a984a930512025-08-20T03:55:00ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017256957710.1155/2010/412194A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage FormsUma Mahesh Karra0Sanjeeva Yarkala1Analytical Research and Development Laboratory, International Specialty Products India Pvt.Ltd., No. 6-3-1090/A, Somajiguda, Hydrabad-500 082, IndiaAnalytical Research and Development Laboratory, International Specialty Products India Pvt.Ltd., No. 6-3-1090/A, Somajiguda, Hydrabad-500 082, IndiaA simple and rapid reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for quantitative determination of rabeprazole in bulk drug samples and formulations. Rabeprazole was analyzed by using reverse phase LC-GC column (Inertsil ODS, 4.6 mm x 25 cm, 5 microns), with mobile phase consisting of methanol: water (78:22 v/v). The flow rate was set 1.0 mL/min and analysis was performed at wavelength 288 nm using Photo Diode Array (PDA) detector at ambient temperature. The method was validated and stability studies were conducted under different conditions. The retention time for rabeprazole was around 4.12 minutes. The calibration curves were linear (r≥0.9998) over a concentration range from 20.0 to 80.0 μg/mL. Limit of detection (LOD) and Limit of quantitation (LOQ) were 8 ng/mL and 24 ng/mL respectively. The developed method was successfully applied to estimate the amount of rabeprazole in tablet formulations.http://dx.doi.org/10.1155/2010/412194 |
| spellingShingle | Uma Mahesh Karra Sanjeeva Yarkala A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms E-Journal of Chemistry |
| title | A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms |
| title_full | A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms |
| title_fullStr | A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms |
| title_full_unstemmed | A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms |
| title_short | A Simple and Validated Reverse Phase HPLC Methodfor the Determination of Rabeprazole inPharmaceutical Dosage Forms |
| title_sort | simple and validated reverse phase hplc methodfor the determination of rabeprazole inpharmaceutical dosage forms |
| url | http://dx.doi.org/10.1155/2010/412194 |
| work_keys_str_mv | AT umamaheshkarra asimpleandvalidatedreversephasehplcmethodforthedeterminationofrabeprazoleinpharmaceuticaldosageforms AT sanjeevayarkala asimpleandvalidatedreversephasehplcmethodforthedeterminationofrabeprazoleinpharmaceuticaldosageforms AT umamaheshkarra simpleandvalidatedreversephasehplcmethodforthedeterminationofrabeprazoleinpharmaceuticaldosageforms AT sanjeevayarkala simpleandvalidatedreversephasehplcmethodforthedeterminationofrabeprazoleinpharmaceuticaldosageforms |