Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model
A degranulation model was established using mouse bone marrow-derived mast cell (BMMC) to investigate the anti-allergic activity of milk exosomes (M-Exo). Casein was removed from cow’s milk using a rennet-assisted method, and then M-Exo was isolated from the milk by ultracentrifugation and character...
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China Food Publishing Company
2025-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-026.pdf |
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| author | FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing |
| author_facet | FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing |
| author_sort | FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing |
| collection | DOAJ |
| description | A degranulation model was established using mouse bone marrow-derived mast cell (BMMC) to investigate the anti-allergic activity of milk exosomes (M-Exo). Casein was removed from cow’s milk using a rennet-assisted method, and then M-Exo was isolated from the milk by ultracentrifugation and characterized. Different concentrations of M-Exo were used to treat sensitized BMMCs, and the release rate of activated β-hexosaminidase (β-HEX) and cytokine secretion were measured. The results showed that the prepared M-Exo had a typical tea tray-like bilayer membrane structure, with particle size ranging from 30 to 200 nm. M-Exo contained exosome marker proteins CD81, tumor susceptibility gene 101 (TSG101) and heatshockprotein 70 (HSP70). It was further found that M-Exo could be taken up by BMMCs. In the degranulation model, it was confirmed that M-Exo could significantly inhibit the release rate of β-HEX and downregulate the expression of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-13 in a dose-dependent manner, indicating that M-Exo has anti-allergic activity. |
| format | Article |
| id | doaj-art-25ea18ede9c04b16ab9e06b336b271f8 |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-25ea18ede9c04b16ab9e06b336b271f82025-08-20T03:12:32ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461222623210.7506/spkx1002-6630-20241104-016Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation ModelFU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing0(1. State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China;2. College of Food Science & Technology, Nanchang University, Nanchang 330047, China;3. Jiangxi Provincial Key Laboratory of Food Allergy, Nanchang University, Nanchang 330047, China;4. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China)A degranulation model was established using mouse bone marrow-derived mast cell (BMMC) to investigate the anti-allergic activity of milk exosomes (M-Exo). Casein was removed from cow’s milk using a rennet-assisted method, and then M-Exo was isolated from the milk by ultracentrifugation and characterized. Different concentrations of M-Exo were used to treat sensitized BMMCs, and the release rate of activated β-hexosaminidase (β-HEX) and cytokine secretion were measured. The results showed that the prepared M-Exo had a typical tea tray-like bilayer membrane structure, with particle size ranging from 30 to 200 nm. M-Exo contained exosome marker proteins CD81, tumor susceptibility gene 101 (TSG101) and heatshockprotein 70 (HSP70). It was further found that M-Exo could be taken up by BMMCs. In the degranulation model, it was confirmed that M-Exo could significantly inhibit the release rate of β-HEX and downregulate the expression of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and IL-13 in a dose-dependent manner, indicating that M-Exo has anti-allergic activity.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-026.pdfmilk exosomes; characterization; mast cells; degranulation; anti-allergic |
| spellingShingle | FU Siqi, LIU Yan, ZHANG Chao, YANG Fan, XIE Ruofan, WANG Xiaodong, LI Xin, CHEN Hongbing Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model Shipin Kexue milk exosomes; characterization; mast cells; degranulation; anti-allergic |
| title | Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model |
| title_full | Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model |
| title_fullStr | Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model |
| title_full_unstemmed | Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model |
| title_short | Evaluation of the Anti-allergic Activity of Bovine Milk Exosomes Using Mast Cell Degranulation Model |
| title_sort | evaluation of the anti allergic activity of bovine milk exosomes using mast cell degranulation model |
| topic | milk exosomes; characterization; mast cells; degranulation; anti-allergic |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-026.pdf |
| work_keys_str_mv | AT fusiqiliuyanzhangchaoyangfanxieruofanwangxiaodonglixinchenhongbing evaluationoftheantiallergicactivityofbovinemilkexosomesusingmastcelldegranulationmodel |