Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease
ObjectiveThis study aimed to evaluate the resistance to blight disease and genetic diversity of different cucumber (Cucumis sativus L.) germplasm resources from around the world, providing a basis and reference for cucumber resistance breeding.MethodThe resistance of these resources was evaluated by...
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South China Agricultural University
2025-09-01
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| Series: | Huanan Nongye Daxue xuebao |
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| Online Access: | https://journal.scau.edu.cn/article/doi/10.7671/j.issn.1001-411X.202501010 |
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| author | Xujia LI Tingquan WU Zeqin LIN Haishun CAO Zhaowan SHI Delong TAN Rui WANG |
| author_facet | Xujia LI Tingquan WU Zeqin LIN Haishun CAO Zhaowan SHI Delong TAN Rui WANG |
| author_sort | Xujia LI |
| collection | DOAJ |
| description | ObjectiveThis study aimed to evaluate the resistance to blight disease and genetic diversity of different cucumber (Cucumis sativus L.) germplasm resources from around the world, providing a basis and reference for cucumber resistance breeding.MethodThe resistance of these resources was evaluated by artificial inoculation with Phytophthora melonis. The important agronomic traits of 90 cucumber germplasm resources were recorded. Two hundred pairs of SSR primers were designed based on the whole genome to screen molecular markers and analyze genetic diversity.ResultDifferent types of cucumber materials had significant differences in blight disease resistance. There were 4 highly resistant materials and 10 resistant materials, most of which were South China type cucumbers. There were 38 susceptible materials and 38 highly susceptible materials. The results of SSR analysis showed that a total of 142 alleles were amplified from 90 cucumber resources using 45 primer pairs, with a range from 2 to 4 alleles and an average of 3.22 alleles amplified per a pair of primers. The effective allele number ranged from 1.0225 to 3.0840 with an average of 1.6684. The Nei’s genetic diversity index ranged from 0.0610 to 1.1728 with an average of 0.5816. The average Shannon diversity index was 0.2402. The genetic similarity coefficient ranged from 0.0556 to 1.0000 with an average of 0.5783. The cluster analysis showed that at the genetic similarity coefficient of 0.5500, these 90 cucumber germplasms could be clustered into 9 groups. The resistant germplasm resources were concentrated in groups Ⅰ, Ⅱ and Ⅺ, predominantly South China type cucumbers. ConclusionThrough artificial inoculation of 90 cucumber materials with P. melonis, 14 resistant materials were identified. The 45 SSR primer pairs with high polymorphism were screened out, which could effectively cluster 90 cucumber germplasm materials. This study provides the materials and theoretical basis for screening and evaluating excellent cucumber germplasm resources and resistance breeding. |
| format | Article |
| id | doaj-art-25d0fad8e3554b3388d3fdcd1e869a45 |
| institution | Kabale University |
| issn | 1001-411X |
| language | zho |
| publishDate | 2025-09-01 |
| publisher | South China Agricultural University |
| record_format | Article |
| series | Huanan Nongye Daxue xuebao |
| spelling | doaj-art-25d0fad8e3554b3388d3fdcd1e869a452025-08-20T03:45:06ZzhoSouth China Agricultural UniversityHuanan Nongye Daxue xuebao1001-411X2025-09-0146572973910.7671/j.issn.1001-411X.202501010202501010Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora diseaseXujia LI0Tingquan WU1Zeqin LIN2Haishun CAO3Zhaowan SHI4Delong TAN5Rui WANG6Institute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Facility Agriculture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaObjectiveThis study aimed to evaluate the resistance to blight disease and genetic diversity of different cucumber (Cucumis sativus L.) germplasm resources from around the world, providing a basis and reference for cucumber resistance breeding.MethodThe resistance of these resources was evaluated by artificial inoculation with Phytophthora melonis. The important agronomic traits of 90 cucumber germplasm resources were recorded. Two hundred pairs of SSR primers were designed based on the whole genome to screen molecular markers and analyze genetic diversity.ResultDifferent types of cucumber materials had significant differences in blight disease resistance. There were 4 highly resistant materials and 10 resistant materials, most of which were South China type cucumbers. There were 38 susceptible materials and 38 highly susceptible materials. The results of SSR analysis showed that a total of 142 alleles were amplified from 90 cucumber resources using 45 primer pairs, with a range from 2 to 4 alleles and an average of 3.22 alleles amplified per a pair of primers. The effective allele number ranged from 1.0225 to 3.0840 with an average of 1.6684. The Nei’s genetic diversity index ranged from 0.0610 to 1.1728 with an average of 0.5816. The average Shannon diversity index was 0.2402. The genetic similarity coefficient ranged from 0.0556 to 1.0000 with an average of 0.5783. The cluster analysis showed that at the genetic similarity coefficient of 0.5500, these 90 cucumber germplasms could be clustered into 9 groups. The resistant germplasm resources were concentrated in groups Ⅰ, Ⅱ and Ⅺ, predominantly South China type cucumbers. ConclusionThrough artificial inoculation of 90 cucumber materials with P. melonis, 14 resistant materials were identified. The 45 SSR primer pairs with high polymorphism were screened out, which could effectively cluster 90 cucumber germplasm materials. This study provides the materials and theoretical basis for screening and evaluating excellent cucumber germplasm resources and resistance breeding.https://journal.scau.edu.cn/article/doi/10.7671/j.issn.1001-411X.202501010cucumis sativus l.germplasm resourcephytophthora diseasessr markergenetic diversitydisease resistance evaluation |
| spellingShingle | Xujia LI Tingquan WU Zeqin LIN Haishun CAO Zhaowan SHI Delong TAN Rui WANG Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease Huanan Nongye Daxue xuebao cucumis sativus l. germplasm resource phytophthora disease ssr marker genetic diversity disease resistance evaluation |
| title | Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease |
| title_full | Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease |
| title_fullStr | Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease |
| title_full_unstemmed | Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease |
| title_short | Resistance identification and genetic diversity analysis of cucumber germplasm resources to Phytophthora disease |
| title_sort | resistance identification and genetic diversity analysis of cucumber germplasm resources to phytophthora disease |
| topic | cucumis sativus l. germplasm resource phytophthora disease ssr marker genetic diversity disease resistance evaluation |
| url | https://journal.scau.edu.cn/article/doi/10.7671/j.issn.1001-411X.202501010 |
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