Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method
Misidentification and cross-contamination of cell lines are major problems of cell cultures that can make scientific results and their reproducibility unreliable. This paper describes a PCR-based method for easily identifying or confirming the species of origin of cell lines by using a panel of olig...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02322rr05 |
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| _version_ | 1850152975517876224 |
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| author | B. Parodi O. Aresu D. Bini R. Lorenzini F. Schena P. Visconti M. Cesaro D. Ferrera V. Andreotti T. Ruzzon |
| author_facet | B. Parodi O. Aresu D. Bini R. Lorenzini F. Schena P. Visconti M. Cesaro D. Ferrera V. Andreotti T. Ruzzon |
| author_sort | B. Parodi |
| collection | DOAJ |
| description | Misidentification and cross-contamination of cell lines are major problems of cell cultures that can make scientific results and their reproducibility unreliable. This paper describes a PCR-based method for easily identifying or confirming the species of origin of cell lines by using a panel of oligonucleotides specific for the nine animal species most common in cell culture laboratories. A panel of 35 human and animal cell lines, whose species of origin were previously confirmed by isoenzyme assay, was studied with nine species-specific primer pairs that specifically anneal to DNA sequences codifying for human, cat, dog, mouse, rat, horse, rabbit, African Green monkey cytochrome c oxidase subunit I (cox I), and one primer pair specific for the cytochrome b gene of Chinese hamster. The amplified fragments were analyzed by electrophoresis in ethidium bromide-stained 2% agarose gels. The method is simple, rapid, highly sensitive, and useful for routinely monitoring the species identity of cell cultures. |
| format | Article |
| id | doaj-art-25bfb2d6834d43dbbc613faeb7fb86ab |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2002-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-25bfb2d6834d43dbbc613faeb7fb86ab2025-08-20T02:25:51ZengTaylor & Francis GroupBioTechniques0736-62051940-98182002-02-0132243244010.2144/02322rr05Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based MethodB. Parodi0O. Aresu1D. Bini2R. Lorenzini3F. Schena4P. Visconti5M. Cesaro6D. Ferrera7V. Andreotti8T. Ruzzon91National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, Italy1National Institute for Cancer Research, Genoa, ItalyMisidentification and cross-contamination of cell lines are major problems of cell cultures that can make scientific results and their reproducibility unreliable. This paper describes a PCR-based method for easily identifying or confirming the species of origin of cell lines by using a panel of oligonucleotides specific for the nine animal species most common in cell culture laboratories. A panel of 35 human and animal cell lines, whose species of origin were previously confirmed by isoenzyme assay, was studied with nine species-specific primer pairs that specifically anneal to DNA sequences codifying for human, cat, dog, mouse, rat, horse, rabbit, African Green monkey cytochrome c oxidase subunit I (cox I), and one primer pair specific for the cytochrome b gene of Chinese hamster. The amplified fragments were analyzed by electrophoresis in ethidium bromide-stained 2% agarose gels. The method is simple, rapid, highly sensitive, and useful for routinely monitoring the species identity of cell cultures.https://www.future-science.com/doi/10.2144/02322rr05 |
| spellingShingle | B. Parodi O. Aresu D. Bini R. Lorenzini F. Schena P. Visconti M. Cesaro D. Ferrera V. Andreotti T. Ruzzon Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method BioTechniques |
| title | Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method |
| title_full | Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method |
| title_fullStr | Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method |
| title_full_unstemmed | Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method |
| title_short | Species Identification and Confirmation of Human and Animal Cell Lines: A PCR-Based Method |
| title_sort | species identification and confirmation of human and animal cell lines a pcr based method |
| url | https://www.future-science.com/doi/10.2144/02322rr05 |
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