Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study

Abstract Background hPG80 (circulating progastrin), initially recognized for its oncogenic properties due to its direct link to the Wnt signaling pathway, is secreted by cancer cells and detectable in the blood of cancer patients. The ONCOPRO centralized case-control study (NCT03787056) was designed...

Full description

Saved in:
Bibliographic Details
Main Authors: Benoit You, Sebastien Couraud, Philippe Ceruse, Lionel Badet, Philippe Paparel, Alice Durand, Marielle Guillet, Philippe Merle, Gaelle Lescuyer, Charles-André Philip, Francois Ducray, Mathieu Pioche, Lionel Karlin, Jean-Christophe Lifante, Olivier Glehen, Pierre-Adrien Bolze, Marion Chauvenet, Carole Langlois-Jacques, Fabien Subtil, Marie Piecyk, Aurore Carrot, Dominique Joubert, Alexandre Prieur, Berengere Vire, Sara Calattini, Lea Payen
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Biomarker Research
Subjects:
Online Access:https://doi.org/10.1186/s40364-025-00793-z
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849402050896461824
author Benoit You
Sebastien Couraud
Philippe Ceruse
Lionel Badet
Philippe Paparel
Alice Durand
Marielle Guillet
Philippe Merle
Gaelle Lescuyer
Charles-André Philip
Francois Ducray
Mathieu Pioche
Lionel Karlin
Jean-Christophe Lifante
Olivier Glehen
Pierre-Adrien Bolze
Marion Chauvenet
Carole Langlois-Jacques
Fabien Subtil
Marie Piecyk
Aurore Carrot
Dominique Joubert
Alexandre Prieur
Berengere Vire
Sara Calattini
Lea Payen
author_facet Benoit You
Sebastien Couraud
Philippe Ceruse
Lionel Badet
Philippe Paparel
Alice Durand
Marielle Guillet
Philippe Merle
Gaelle Lescuyer
Charles-André Philip
Francois Ducray
Mathieu Pioche
Lionel Karlin
Jean-Christophe Lifante
Olivier Glehen
Pierre-Adrien Bolze
Marion Chauvenet
Carole Langlois-Jacques
Fabien Subtil
Marie Piecyk
Aurore Carrot
Dominique Joubert
Alexandre Prieur
Berengere Vire
Sara Calattini
Lea Payen
author_sort Benoit You
collection DOAJ
description Abstract Background hPG80 (circulating progastrin), initially recognized for its oncogenic properties due to its direct link to the Wnt signaling pathway, is secreted by cancer cells and detectable in the blood of cancer patients. The ONCOPRO centralized case-control study (NCT03787056) was designed to prospectively evaluate the diagnostic utility of hPG80 in patients with 16 different types of cancer. Methods hPG80 levels were measured in 421 patients with 16 newly diagnosed cancers (median age 65.6 years old) using the DxPG80.Lab kit (Biodena Care). The diagnostic performance of hPG80 (the primary endpoint) was assessed by comparing baseline hPG80 levels in cancer patients with those of 330 asymptomatic aged-matched healthy subjects from the general population. Results Between 2018 and 2022, a total of 506 cancer patients were enrolled in the study, with 421 assessable across 16 distinct cancer cohorts. hPG80 concentrations were significantly higher in cancer patients compared to the healthy population (median 3.8 [IQR: 1.0-11.1] vs. 1.9 [IQR: 0.6–4.2] pM, P < 0.0001). hPG80 levels were not impacted by renal failure, liver dysfunction, or cancer-associated inflammation. The diagnostic accuracy in cancer patients was ROC AUC 0.63, 95% CI = [0.59–0.67]. The highest diagnostic accuracy was seen in patients with lung cancer (AUC 0.75, 95% CI [0.68–0.82]; specificity = 88% for hPG80 > 7.73 pM in patients aged > 58 years old) and hepatocellular carcinoma HCC (ROC AUC 0.75, 95% CI [0.66-083]; specificity = 88% for hPG80 > 7.73 pM in patients aged > 58 years old). Conclusions This large prospective study confirms that cancer patients have significantly higher hPG80 blood concentration compared to the healthy population. Incorporating this straightforward ELISA assay into screening programs is warranted. Trial registration NCT03787056.
format Article
id doaj-art-25b373bb2362477ebeef50bd868652d1
institution Kabale University
issn 2050-7771
language English
publishDate 2025-07-01
publisher BMC
record_format Article
series Biomarker Research
spelling doaj-art-25b373bb2362477ebeef50bd868652d12025-08-20T03:37:38ZengBMCBiomarker Research2050-77712025-07-0113111110.1186/s40364-025-00793-zDiagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective studyBenoit You0Sebastien Couraud1Philippe Ceruse2Lionel Badet3Philippe Paparel4Alice Durand5Marielle Guillet6Philippe Merle7Gaelle Lescuyer8Charles-André Philip9Francois Ducray10Mathieu Pioche11Lionel Karlin12Jean-Christophe Lifante13Olivier Glehen14Pierre-Adrien Bolze15Marion Chauvenet16Carole Langlois-Jacques17Fabien Subtil18Marie Piecyk19Aurore Carrot20Dominique Joubert21Alexandre Prieur22Berengere Vire23Sara Calattini24Lea Payen25Lyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityProgastrin ManufacturingProgastrin ManufacturingProgastrin ManufacturingLyon University Hospital, IC-HCL, Lyon UniversityLyon University Hospital, IC-HCL, Lyon UniversityAbstract Background hPG80 (circulating progastrin), initially recognized for its oncogenic properties due to its direct link to the Wnt signaling pathway, is secreted by cancer cells and detectable in the blood of cancer patients. The ONCOPRO centralized case-control study (NCT03787056) was designed to prospectively evaluate the diagnostic utility of hPG80 in patients with 16 different types of cancer. Methods hPG80 levels were measured in 421 patients with 16 newly diagnosed cancers (median age 65.6 years old) using the DxPG80.Lab kit (Biodena Care). The diagnostic performance of hPG80 (the primary endpoint) was assessed by comparing baseline hPG80 levels in cancer patients with those of 330 asymptomatic aged-matched healthy subjects from the general population. Results Between 2018 and 2022, a total of 506 cancer patients were enrolled in the study, with 421 assessable across 16 distinct cancer cohorts. hPG80 concentrations were significantly higher in cancer patients compared to the healthy population (median 3.8 [IQR: 1.0-11.1] vs. 1.9 [IQR: 0.6–4.2] pM, P < 0.0001). hPG80 levels were not impacted by renal failure, liver dysfunction, or cancer-associated inflammation. The diagnostic accuracy in cancer patients was ROC AUC 0.63, 95% CI = [0.59–0.67]. The highest diagnostic accuracy was seen in patients with lung cancer (AUC 0.75, 95% CI [0.68–0.82]; specificity = 88% for hPG80 > 7.73 pM in patients aged > 58 years old) and hepatocellular carcinoma HCC (ROC AUC 0.75, 95% CI [0.66-083]; specificity = 88% for hPG80 > 7.73 pM in patients aged > 58 years old). Conclusions This large prospective study confirms that cancer patients have significantly higher hPG80 blood concentration compared to the healthy population. Incorporating this straightforward ELISA assay into screening programs is warranted. Trial registration NCT03787056.https://doi.org/10.1186/s40364-025-00793-zBiomarkers, tumorEarly detection of CancerWnt signaling pathwayProspective studies
spellingShingle Benoit You
Sebastien Couraud
Philippe Ceruse
Lionel Badet
Philippe Paparel
Alice Durand
Marielle Guillet
Philippe Merle
Gaelle Lescuyer
Charles-André Philip
Francois Ducray
Mathieu Pioche
Lionel Karlin
Jean-Christophe Lifante
Olivier Glehen
Pierre-Adrien Bolze
Marion Chauvenet
Carole Langlois-Jacques
Fabien Subtil
Marie Piecyk
Aurore Carrot
Dominique Joubert
Alexandre Prieur
Berengere Vire
Sara Calattini
Lea Payen
Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
Biomarker Research
Biomarkers, tumor
Early detection of Cancer
Wnt signaling pathway
Prospective studies
title Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
title_full Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
title_fullStr Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
title_full_unstemmed Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
title_short Diagnostic value of the wnt target and cancer-associated blood biomarker hPG80: ONCOPRO case-control prospective study
title_sort diagnostic value of the wnt target and cancer associated blood biomarker hpg80 oncopro case control prospective study
topic Biomarkers, tumor
Early detection of Cancer
Wnt signaling pathway
Prospective studies
url https://doi.org/10.1186/s40364-025-00793-z
work_keys_str_mv AT benoityou diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT sebastiencouraud diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT philippeceruse diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT lionelbadet diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT philippepaparel diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT alicedurand diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT marielleguillet diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT philippemerle diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT gaellelescuyer diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT charlesandrephilip diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT francoisducray diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT mathieupioche diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT lionelkarlin diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT jeanchristophelifante diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT olivierglehen diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT pierreadrienbolze diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT marionchauvenet diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT carolelangloisjacques diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT fabiensubtil diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT mariepiecyk diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT aurorecarrot diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT dominiquejoubert diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT alexandreprieur diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT berengerevire diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT saracalattini diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy
AT leapayen diagnosticvalueofthewnttargetandcancerassociatedbloodbiomarkerhpg80oncoprocasecontrolprospectivestudy