Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity
Background: The most effective approach to control and prevent rabies is by proper pre- and post-exposure immunization. Glycoprotein (G) is a structural protein of the rabies virus that plays a crucial function in host protection against the virus. This study assessed the effects of integrating an e...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-07-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | https://journals.lww.com/10.4103/abr.abr_480_24 |
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| author | Ashkan Alamdary Alireza Gholami Mohammad Azizi Zahra Noormohammadi |
| author_facet | Ashkan Alamdary Alireza Gholami Mohammad Azizi Zahra Noormohammadi |
| author_sort | Ashkan Alamdary |
| collection | DOAJ |
| description | Background:
The most effective approach to control and prevent rabies is by proper pre- and post-exposure immunization. Glycoprotein (G) is a structural protein of the rabies virus that plays a crucial function in host protection against the virus. This study assessed the effects of integrating an extra copy of the glycoprotein gene into the rabies virus genome using reverse genetics on the immunogenicity and titer of the recombinant virus.
Materials and Methods:
The expression of the recombinant viral glycoprotein was compared to the PV strain using qRT-PCR and western blot techniques. The virus titers were studied using a fluorescent antibody test (FAT). The inactivated recombinant virus was administered to BALB/c mice as a vaccine, and the immunogenicity was assessed using the rapid fluorescent focus inhibition test (RFFIT).
Results:
Glycoprotein overexpression was observed using qRT-PCR (2.47-fold increase) and confirmed by western blot analysis (3.4-fold increase). The double G virus showed significantly higher virus titers than the PV strain. The immunogenicity of the double G virus was also considerably increased. The VNA titers induced by the double G virus were approximately two and three times higher at 14 and 21 days post-inoculation, respectively.
Conclusions:
Based on our findings, the PV strain’s G gene duplication showed greater viral titers, better VNA induction, and higher G expression levels. Both the development of rabies vaccines and the investigation of various cellular processes involved in the virus’s life cycle may be advanced by characterizing the recombinant double G strain. |
| format | Article |
| id | doaj-art-256bdcd7fda14e3f8b409ddf104467c2 |
| institution | Kabale University |
| issn | 2277-9175 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj-art-256bdcd7fda14e3f8b409ddf104467c22025-08-20T03:39:15ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752025-07-01141757510.4103/abr.abr_480_24Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and ImmunogenicityAshkan AlamdaryAlireza GholamiMohammad AziziZahra NoormohammadiBackground: The most effective approach to control and prevent rabies is by proper pre- and post-exposure immunization. Glycoprotein (G) is a structural protein of the rabies virus that plays a crucial function in host protection against the virus. This study assessed the effects of integrating an extra copy of the glycoprotein gene into the rabies virus genome using reverse genetics on the immunogenicity and titer of the recombinant virus. Materials and Methods: The expression of the recombinant viral glycoprotein was compared to the PV strain using qRT-PCR and western blot techniques. The virus titers were studied using a fluorescent antibody test (FAT). The inactivated recombinant virus was administered to BALB/c mice as a vaccine, and the immunogenicity was assessed using the rapid fluorescent focus inhibition test (RFFIT). Results: Glycoprotein overexpression was observed using qRT-PCR (2.47-fold increase) and confirmed by western blot analysis (3.4-fold increase). The double G virus showed significantly higher virus titers than the PV strain. The immunogenicity of the double G virus was also considerably increased. The VNA titers induced by the double G virus were approximately two and three times higher at 14 and 21 days post-inoculation, respectively. Conclusions: Based on our findings, the PV strain’s G gene duplication showed greater viral titers, better VNA induction, and higher G expression levels. Both the development of rabies vaccines and the investigation of various cellular processes involved in the virus’s life cycle may be advanced by characterizing the recombinant double G strain.https://journals.lww.com/10.4103/abr.abr_480_24glycoproteinimmunogenicitypv strainrabies virusvirus titer |
| spellingShingle | Ashkan Alamdary Alireza Gholami Mohammad Azizi Zahra Noormohammadi Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity Advanced Biomedical Research glycoprotein immunogenicity pv strain rabies virus virus titer |
| title | Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity |
| title_full | Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity |
| title_fullStr | Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity |
| title_full_unstemmed | Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity |
| title_short | Duplication of the Rabies Virus Glycoprotein Enhances the Virus Propagation Rate and Immunogenicity |
| title_sort | duplication of the rabies virus glycoprotein enhances the virus propagation rate and immunogenicity |
| topic | glycoprotein immunogenicity pv strain rabies virus virus titer |
| url | https://journals.lww.com/10.4103/abr.abr_480_24 |
| work_keys_str_mv | AT ashkanalamdary duplicationoftherabiesvirusglycoproteinenhancestheviruspropagationrateandimmunogenicity AT alirezagholami duplicationoftherabiesvirusglycoproteinenhancestheviruspropagationrateandimmunogenicity AT mohammadazizi duplicationoftherabiesvirusglycoproteinenhancestheviruspropagationrateandimmunogenicity AT zahranoormohammadi duplicationoftherabiesvirusglycoproteinenhancestheviruspropagationrateandimmunogenicity |