Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders

Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (𝑛=2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunos...

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Main Authors: R. L. Luciano, A. L. S. P. Cardoso, G. F. Z. Stoppa, A. M. I. Kanashiro, A. G. M. de Castro, E. N. C. Tessari
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:Veterinary Medicine International
Online Access:http://dx.doi.org/10.4061/2011/304349
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author R. L. Luciano
A. L. S. P. Cardoso
G. F. Z. Stoppa
A. M. I. Kanashiro
A. G. M. de Castro
E. N. C. Tessari
author_facet R. L. Luciano
A. L. S. P. Cardoso
G. F. Z. Stoppa
A. M. I. Kanashiro
A. G. M. de Castro
E. N. C. Tessari
author_sort R. L. Luciano
collection DOAJ
description Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (𝑛=2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (𝑃≤.001), and Kappa coefficient showed a weak correlation between the two techniques (𝑘=0.25; 0.21≤𝑘<0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.
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publishDate 2011-01-01
publisher Wiley
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series Veterinary Medicine International
spelling doaj-art-2555b850a2f44aa5af15e21f7cbe336c2025-02-03T05:46:22ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/304349304349Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry BreedersR. L. Luciano0A. L. S. P. Cardoso1G. F. Z. Stoppa2A. M. I. Kanashiro3A. G. M. de Castro4E. N. C. Tessari5Centro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilCentro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilCentro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilCentro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilCentro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilCentro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, BrazilAvian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (𝑛=2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (𝑃≤.001), and Kappa coefficient showed a weak correlation between the two techniques (𝑘=0.25; 0.21≤𝑘<0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.http://dx.doi.org/10.4061/2011/304349
spellingShingle R. L. Luciano
A. L. S. P. Cardoso
G. F. Z. Stoppa
A. M. I. Kanashiro
A. G. M. de Castro
E. N. C. Tessari
Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
Veterinary Medicine International
title Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
title_full Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
title_fullStr Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
title_full_unstemmed Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
title_short Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders
title_sort comparative study of serological tests for mycoplasma synoviae diagnosis in commercial poultry breeders
url http://dx.doi.org/10.4061/2011/304349
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