Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression
Pneumonia virus of mice (PVM) infection is a reference animal model for human respiratory syncytial virus (hRSV), a leading cause of lower respiratory tract disease in children under 5 years of age and in the elderly. This longitudinal study employed necropsy to examine macroscopic lesions, histolog...
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2025-04-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/4/548 |
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| author | Etienne Levy Gautier Gilliaux Michaël Sarlet Daniel Desmecht Anne-Sophie Van Laere |
| author_facet | Etienne Levy Gautier Gilliaux Michaël Sarlet Daniel Desmecht Anne-Sophie Van Laere |
| author_sort | Etienne Levy |
| collection | DOAJ |
| description | Pneumonia virus of mice (PVM) infection is a reference animal model for human respiratory syncytial virus (hRSV), a leading cause of lower respiratory tract disease in children under 5 years of age and in the elderly. This longitudinal study employed necropsy to examine macroscopic lesions, histological slides to assess microscopic lesions, and qRT-PCR to measure lung viral load and cytokine expression in PVM-infected mice from three different genetic backgrounds, spanning from day 1 to day 6 post-infection. Our analysis reveals a strong correlation between viral load and microscopic lesions across the 129/Sv, BALB/c, and SJL/J mouse lines, indicating that PVM pathogenicity is partially driven by the virus itself. Additionally, a significant correlation between cytokine levels and lesion severity was observed in 129/Sv and BALB/c mice, suggesting an important role of cytokines in disease progression. This study emphasizes the interplay between viral load and cytokine-driven tissue damage, with genetic background significantly influencing disease outcomes. |
| format | Article |
| id | doaj-art-254dc5c520d849d7a096e8bd29de8a55 |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-254dc5c520d849d7a096e8bd29de8a552025-08-20T02:18:11ZengMDPI AGViruses1999-49152025-04-0117454810.3390/v17040548Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine ExpressionEtienne Levy0Gautier Gilliaux1Michaël Sarlet2Daniel Desmecht3Anne-Sophie Van Laere4Department of Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, BelgiumDepartment of Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, BelgiumDepartment of Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, BelgiumDepartment of Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, BelgiumDepartment of Pathology, FARAH Research Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, BelgiumPneumonia virus of mice (PVM) infection is a reference animal model for human respiratory syncytial virus (hRSV), a leading cause of lower respiratory tract disease in children under 5 years of age and in the elderly. This longitudinal study employed necropsy to examine macroscopic lesions, histological slides to assess microscopic lesions, and qRT-PCR to measure lung viral load and cytokine expression in PVM-infected mice from three different genetic backgrounds, spanning from day 1 to day 6 post-infection. Our analysis reveals a strong correlation between viral load and microscopic lesions across the 129/Sv, BALB/c, and SJL/J mouse lines, indicating that PVM pathogenicity is partially driven by the virus itself. Additionally, a significant correlation between cytokine levels and lesion severity was observed in 129/Sv and BALB/c mice, suggesting an important role of cytokines in disease progression. This study emphasizes the interplay between viral load and cytokine-driven tissue damage, with genetic background significantly influencing disease outcomes.https://www.mdpi.com/1999-4915/17/4/548pneumonia virus of micerespiratory syncytial viruscytokinemouse line |
| spellingShingle | Etienne Levy Gautier Gilliaux Michaël Sarlet Daniel Desmecht Anne-Sophie Van Laere Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression Viruses pneumonia virus of mice respiratory syncytial virus cytokine mouse line |
| title | Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression |
| title_full | Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression |
| title_fullStr | Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression |
| title_full_unstemmed | Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression |
| title_short | Host-Strain-Specific Responses to Pneumonia Virus of Mice Infection: A Study of Lesions, Viral Load, and Cytokine Expression |
| title_sort | host strain specific responses to pneumonia virus of mice infection a study of lesions viral load and cytokine expression |
| topic | pneumonia virus of mice respiratory syncytial virus cytokine mouse line |
| url | https://www.mdpi.com/1999-4915/17/4/548 |
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