TLC determination of some flavanones in the buds of different genus Populus species and hybrids
Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar...
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| Format: | Article |
| Language: | English |
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Sciendo
2018-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0018 |
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| author | Pobłocka-Olech Loretta Migas Piotr Krauze-Baranowska Mirosława |
| author_facet | Pobłocka-Olech Loretta Migas Piotr Krauze-Baranowska Mirosława |
| author_sort | Pobłocka-Olech Loretta |
| collection | DOAJ |
| description | Flavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. × canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.). |
| format | Article |
| id | doaj-art-254200d492d9465d950528eed7a285e2 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-254200d492d9465d950528eed7a285e22025-02-02T15:21:58ZengSciendoActa Pharmaceutica1846-95582018-06-0168219921010.2478/acph-2018-0018acph-2018-0018TLC determination of some flavanones in the buds of different genus Populus species and hybridsPobłocka-Olech Loretta0Migas Piotr1Krauze-Baranowska Mirosława2Department of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk80-416Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk80-416Gdańsk, PolandDepartment of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk80-416Gdańsk, PolandFlavonoids in the buds of eight Populus species and hybrids were detected and compared with the aid of an optimized TLC method. Separation of 17 flavonoid aglycones belonging to different groups, namely, flavones, flavonols, flavanones and flavanonols, previously described as constituents of poplar buds, was performed on silica gel plates using a hexane/ethyl acetate/formic acid (60:40:1.3, V/V/V) mixture as the mobile phase. Pinocembrin and pinostrobin were found in the majority of analyzed poplar buds. For quantitative analysis of both compounds, two TLC evaluation modes, densitometric and videodensitometric, were compared and the established methods were validated. Concentrations of flavanones in some extracts differed slightly or significantly due to the analyzed plant matrix complexity and the TLC evaluation mode applied. Poplar buds rich in flavanones originated from P. × canadensis ‘Robusta’ (1.82 and 2.23 g per 100 g, resp.) and P. balsamifera (1.17 and 2.24 g per 100 g, resp.).https://doi.org/10.2478/acph-2018-0018poplar budspinocembrinpinostrobintlcvideo-densitometrydensitometry |
| spellingShingle | Pobłocka-Olech Loretta Migas Piotr Krauze-Baranowska Mirosława TLC determination of some flavanones in the buds of different genus Populus species and hybrids Acta Pharmaceutica poplar buds pinocembrin pinostrobin tlc video-densitometry densitometry |
| title | TLC determination of some flavanones in the buds of different genus Populus species and hybrids |
| title_full | TLC determination of some flavanones in the buds of different genus Populus species and hybrids |
| title_fullStr | TLC determination of some flavanones in the buds of different genus Populus species and hybrids |
| title_full_unstemmed | TLC determination of some flavanones in the buds of different genus Populus species and hybrids |
| title_short | TLC determination of some flavanones in the buds of different genus Populus species and hybrids |
| title_sort | tlc determination of some flavanones in the buds of different genus populus species and hybrids |
| topic | poplar buds pinocembrin pinostrobin tlc video-densitometry densitometry |
| url | https://doi.org/10.2478/acph-2018-0018 |
| work_keys_str_mv | AT pobłockaolechloretta tlcdeterminationofsomeflavanonesinthebudsofdifferentgenuspopulusspeciesandhybrids AT migaspiotr tlcdeterminationofsomeflavanonesinthebudsofdifferentgenuspopulusspeciesandhybrids AT krauzebaranowskamirosława tlcdeterminationofsomeflavanonesinthebudsofdifferentgenuspopulusspeciesandhybrids |