A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control

Bone regeneration remains a critical challenge in modern medicine. Recent advancements have focused on incorporating hierarchical microstructures into biomaterials to enhance osteogenesis. Mineralized hydrogels, while promising, face limitations in precise microstructure control due to technical com...

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Main Authors: Yun Wang, Chao Fang, Li-Bo Mao, Yan-Hui-Zhi Feng, Yu-Feng Meng, Hai-Cheng Wang, Shu-Hong Yu, Zuo-Lin Wang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Materials Today Bio
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590006425003357
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author Yun Wang
Chao Fang
Li-Bo Mao
Yan-Hui-Zhi Feng
Yu-Feng Meng
Hai-Cheng Wang
Shu-Hong Yu
Zuo-Lin Wang
author_facet Yun Wang
Chao Fang
Li-Bo Mao
Yan-Hui-Zhi Feng
Yu-Feng Meng
Hai-Cheng Wang
Shu-Hong Yu
Zuo-Lin Wang
author_sort Yun Wang
collection DOAJ
description Bone regeneration remains a critical challenge in modern medicine. Recent advancements have focused on incorporating hierarchical microstructures into biomaterials to enhance osteogenesis. Mineralized hydrogels, while promising, face limitations in precise microstructure control due to technical complexities. In this study, we present a biomimetic hierarchical structural mineralized hydrogel featuring a Liesegang pattern. In vitro experiments confirm that it significantly promotes the migration and osteogenic differentiation of bone mesenchymal stem cells (BMSCs). In vivo experiments further demonstrate its ability to significantly promote bone regeneration, with newly formed bone closely replicating the hydrogel's architecture. Notably, this hydrogel synthesis strategy eliminates time-consuming fabrication and extensive post-processing, offering a scalable and efficient route for advanced bone-regenerative materials.
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publishDate 2025-06-01
publisher Elsevier
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series Materials Today Bio
spelling doaj-art-253cd3d0f3db4d4b816249837a5ade9d2025-08-20T02:12:07ZengElsevierMaterials Today Bio2590-00642025-06-013210177510.1016/j.mtbio.2025.101775A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure controlYun Wang0Chao Fang1Li-Bo Mao2Yan-Hui-Zhi Feng3Yu-Feng Meng4Hai-Cheng Wang5Shu-Hong Yu6Zuo-Lin Wang7Department of Oral Implantology and Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, ChinaDepartment of Orthopedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, ChinaNew Cornerstone Science Laboratory, Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, ChinaDepartment of Oral Implantology and Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, ChinaNew Cornerstone Science Laboratory, Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China; Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, 7610001, IsraelDepartment of Oral Implantology and Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, ChinaNew Cornerstone Science Laboratory, Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China; Institute of Innovative Materials, Department of Chemistry, Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; Corresponding author. New Cornerstone Science Laboratory, Department of Chemistry, Institute of Biomimetic Materials & Chemistry, Anhui Engineering Laboratory of Biomimetic Materials, Division of Nanomaterials & Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.Department of Oral Implantology and Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, China; Corresponding author. Department of Oral Implantology and Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, China.Bone regeneration remains a critical challenge in modern medicine. Recent advancements have focused on incorporating hierarchical microstructures into biomaterials to enhance osteogenesis. Mineralized hydrogels, while promising, face limitations in precise microstructure control due to technical complexities. In this study, we present a biomimetic hierarchical structural mineralized hydrogel featuring a Liesegang pattern. In vitro experiments confirm that it significantly promotes the migration and osteogenic differentiation of bone mesenchymal stem cells (BMSCs). In vivo experiments further demonstrate its ability to significantly promote bone regeneration, with newly formed bone closely replicating the hydrogel's architecture. Notably, this hydrogel synthesis strategy eliminates time-consuming fabrication and extensive post-processing, offering a scalable and efficient route for advanced bone-regenerative materials.http://www.sciencedirect.com/science/article/pii/S2590006425003357Liesegang patternBiomimetic structureBiomimetic mineralizationAlginateMineralized hydrogelBone regeneration
spellingShingle Yun Wang
Chao Fang
Li-Bo Mao
Yan-Hui-Zhi Feng
Yu-Feng Meng
Hai-Cheng Wang
Shu-Hong Yu
Zuo-Lin Wang
A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
Materials Today Bio
Liesegang pattern
Biomimetic structure
Biomimetic mineralization
Alginate
Mineralized hydrogel
Bone regeneration
title A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
title_full A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
title_fullStr A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
title_full_unstemmed A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
title_short A novel Liesegang-patterned mineralized hydrogel drives bone regeneration with microstructure control
title_sort novel liesegang patterned mineralized hydrogel drives bone regeneration with microstructure control
topic Liesegang pattern
Biomimetic structure
Biomimetic mineralization
Alginate
Mineralized hydrogel
Bone regeneration
url http://www.sciencedirect.com/science/article/pii/S2590006425003357
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