Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities
Although osteoarthritis (OA), a degenerative joint disease characterized by the degradation of joint articular cartilage and subchondral bones, is generally regarded as a degenerative rather than inflammatory disease, recent studies have indicated the involvement of inflammation in OA pathogenesis....
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/3619879 |
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| author | Jae Gwang Park Young-Su Yi Yo Han Hong Sulgi Yoo Sang Yun Han Eunji Kim Seong-Gu Jeong Adithan Aravinthan Kwang Soo Baik Su Young Choi Young-Jin Son Jong-Hoon Kim Jae Youl Cho |
| author_facet | Jae Gwang Park Young-Su Yi Yo Han Hong Sulgi Yoo Sang Yun Han Eunji Kim Seong-Gu Jeong Adithan Aravinthan Kwang Soo Baik Su Young Choi Young-Jin Son Jong-Hoon Kim Jae Youl Cho |
| author_sort | Jae Gwang Park |
| collection | DOAJ |
| description | Although osteoarthritis (OA), a degenerative joint disease characterized by the degradation of joint articular cartilage and subchondral bones, is generally regarded as a degenerative rather than inflammatory disease, recent studies have indicated the involvement of inflammation in OA pathogenesis. Tabebuia avellanedae has long been used to treat various diseases; however, its role in inflammatory response and the underlying molecular mechanisms remain poorly understood. In this study, the pharmacological effects of Tabetri (Tabebuia avellanedae ethanol extract (Ta-EE)) on OA pathogenesis induced by monoiodoacetate (MIA) and the underlying mechanisms were investigated using experiments with a rat model and in vitro cellular models. In the animal model, Ta-EE significantly ameliorated OA symptoms and reduced the serum levels of inflammatory mediators and proinflammatory cytokines without any toxicity. The anti-inflammatory activity of Ta-EE was further confirmed in a macrophage-like cell line (RAW264.7). Ta-EE dramatically suppressed the production and mRNA expressions of inflammatory mediators and proinflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 cells without any cytotoxicity. Finally, the chondroprotective effect of Ta-EE was examined in a chondrosarcoma cell line (SW1353). Ta-EE markedly suppressed the mRNA expression of matrix metalloproteinase genes. The anti-inflammatory and chondroprotective activities of Ta-EE were attributed to the targeting of the nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) signaling pathways in macrophages and chondrocytes. |
| format | Article |
| id | doaj-art-25320eb87b6e44ea97c3ebbba0d0eaa0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-25320eb87b6e44ea97c3ebbba0d0eaa02025-02-03T05:47:41ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/36198793619879Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective ActivitiesJae Gwang Park0Young-Su Yi1Yo Han Hong2Sulgi Yoo3Sang Yun Han4Eunji Kim5Seong-Gu Jeong6Adithan Aravinthan7Kwang Soo Baik8Su Young Choi9Young-Jin Son10Jong-Hoon Kim11Jae Youl Cho12Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Pharmaceutical Engineering, Cheongju University, Cheongju 28503, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaCollege of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of KoreaR&I Planning Department, Nutribiotech Co. Ltd., Seoul 06132, Republic of KoreaR&I Planning Department, Nutribiotech Co. Ltd., Seoul 06132, Republic of KoreaDepartment of Pharmacy, Sunchon National University, Suncheon 57922, Republic of KoreaCollege of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaAlthough osteoarthritis (OA), a degenerative joint disease characterized by the degradation of joint articular cartilage and subchondral bones, is generally regarded as a degenerative rather than inflammatory disease, recent studies have indicated the involvement of inflammation in OA pathogenesis. Tabebuia avellanedae has long been used to treat various diseases; however, its role in inflammatory response and the underlying molecular mechanisms remain poorly understood. In this study, the pharmacological effects of Tabetri (Tabebuia avellanedae ethanol extract (Ta-EE)) on OA pathogenesis induced by monoiodoacetate (MIA) and the underlying mechanisms were investigated using experiments with a rat model and in vitro cellular models. In the animal model, Ta-EE significantly ameliorated OA symptoms and reduced the serum levels of inflammatory mediators and proinflammatory cytokines without any toxicity. The anti-inflammatory activity of Ta-EE was further confirmed in a macrophage-like cell line (RAW264.7). Ta-EE dramatically suppressed the production and mRNA expressions of inflammatory mediators and proinflammatory cytokines in lipopolysaccharide-stimulated RAW264.7 cells without any cytotoxicity. Finally, the chondroprotective effect of Ta-EE was examined in a chondrosarcoma cell line (SW1353). Ta-EE markedly suppressed the mRNA expression of matrix metalloproteinase genes. The anti-inflammatory and chondroprotective activities of Ta-EE were attributed to the targeting of the nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) signaling pathways in macrophages and chondrocytes.http://dx.doi.org/10.1155/2017/3619879 |
| spellingShingle | Jae Gwang Park Young-Su Yi Yo Han Hong Sulgi Yoo Sang Yun Han Eunji Kim Seong-Gu Jeong Adithan Aravinthan Kwang Soo Baik Su Young Choi Young-Jin Son Jong-Hoon Kim Jae Youl Cho Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities Mediators of Inflammation |
| title | Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities |
| title_full | Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities |
| title_fullStr | Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities |
| title_full_unstemmed | Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities |
| title_short | Tabetri™ (Tabebuia avellanedae Ethanol Extract) Ameliorates Osteoarthritis Symptoms Induced by Monoiodoacetate through Its Anti-Inflammatory and Chondroprotective Activities |
| title_sort | tabetri™ tabebuia avellanedae ethanol extract ameliorates osteoarthritis symptoms induced by monoiodoacetate through its anti inflammatory and chondroprotective activities |
| url | http://dx.doi.org/10.1155/2017/3619879 |
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