Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ
The study of bacteriophage (phage) gene products and their effects on the host helps to better understand the phage-host relationship and provides clues for the development of new antimicrobial proteins. In this study, we focused on a small protein named Icd with 73 amino acids from phage P1. It inh...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533694/full |
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| author | Kairui Zhao Shuheng Du Linlin Tian Shenping Wang Runqin Shi Haiyu Sun Yao Zhou Chenhao Huang Yanmei Sun Yanmei Sun Shiwei Wang Shiwei Wang Yaodong Chen Yaodong Chen |
| author_facet | Kairui Zhao Shuheng Du Linlin Tian Shenping Wang Runqin Shi Haiyu Sun Yao Zhou Chenhao Huang Yanmei Sun Yanmei Sun Shiwei Wang Shiwei Wang Yaodong Chen Yaodong Chen |
| author_sort | Kairui Zhao |
| collection | DOAJ |
| description | The study of bacteriophage (phage) gene products and their effects on the host helps to better understand the phage-host relationship and provides clues for the development of new antimicrobial proteins. In this study, we focused on a small protein named Icd with 73 amino acids from phage P1. It inhibits the growth of Escherichia coli and rapidly blocks the formation of Z-ring. The results of bacterial two-hybrid and pull-down experiments showed that Icd directly targets FtsZ, a key protein in bacterial division. Furthermore, we identified the core region of Icd as amino acids 12–51; this 40-amino acid protein had similar antibacterial activity to the full-length Icd, inhibiting bacterial growth and division. |
| format | Article |
| id | doaj-art-2528fa6809ec4881aef1034abd78993d |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-2528fa6809ec4881aef1034abd78993d2025-08-20T02:45:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15336941533694Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZKairui Zhao0Shuheng Du1Linlin Tian2Shenping Wang3Runqin Shi4Haiyu Sun5Yao Zhou6Chenhao Huang7Yanmei Sun8Yanmei Sun9Shiwei Wang10Shiwei Wang11Yaodong Chen12Yaodong Chen13Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaProvincial Key Laboratory of Biotechnology of Shaanxi Province, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaProvincial Key Laboratory of Biotechnology of Shaanxi Province, Northwest University, Xi’an, ChinaKey Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi’an, ChinaProvincial Key Laboratory of Biotechnology of Shaanxi Province, Northwest University, Xi’an, ChinaThe study of bacteriophage (phage) gene products and their effects on the host helps to better understand the phage-host relationship and provides clues for the development of new antimicrobial proteins. In this study, we focused on a small protein named Icd with 73 amino acids from phage P1. It inhibits the growth of Escherichia coli and rapidly blocks the formation of Z-ring. The results of bacterial two-hybrid and pull-down experiments showed that Icd directly targets FtsZ, a key protein in bacterial division. Furthermore, we identified the core region of Icd as amino acids 12–51; this 40-amino acid protein had similar antibacterial activity to the full-length Icd, inhibiting bacterial growth and division.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533694/fullbacteriophage P1ICDbacterial cell divisionFtsZphage-host |
| spellingShingle | Kairui Zhao Shuheng Du Linlin Tian Shenping Wang Runqin Shi Haiyu Sun Yao Zhou Chenhao Huang Yanmei Sun Yanmei Sun Shiwei Wang Shiwei Wang Yaodong Chen Yaodong Chen Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ Frontiers in Microbiology bacteriophage P1 ICD bacterial cell division FtsZ phage-host |
| title | Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ |
| title_full | Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ |
| title_fullStr | Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ |
| title_full_unstemmed | Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ |
| title_short | Bacteriophage P1 protein Icd inhibits bacterial division by targeting FtsZ |
| title_sort | bacteriophage p1 protein icd inhibits bacterial division by targeting ftsz |
| topic | bacteriophage P1 ICD bacterial cell division FtsZ phage-host |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1533694/full |
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