Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.

This study evaluates Salmonella contamination in feed mill production facilities over a 12-year period, analyzing collection events from 12 facilities predominantly located in the southeastern United States. The genomic data reveals a historical contamination rate, with 20% of collection events test...

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Main Authors: Johnathan Likens, Jon W Bell, Patricia Rabideau, Stephanie Haynes, Steven Wilson, Sandra Tallent, Eric W Brown, Julie Haendiges
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0327222
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author Johnathan Likens
Jon W Bell
Patricia Rabideau
Stephanie Haynes
Steven Wilson
Sandra Tallent
Eric W Brown
Julie Haendiges
author_facet Johnathan Likens
Jon W Bell
Patricia Rabideau
Stephanie Haynes
Steven Wilson
Sandra Tallent
Eric W Brown
Julie Haendiges
author_sort Johnathan Likens
collection DOAJ
description This study evaluates Salmonella contamination in feed mill production facilities over a 12-year period, analyzing collection events from 12 facilities predominantly located in the southeastern United States. The genomic data reveals a historical contamination rate, with 20% of collection events testing positive for Salmonella. Utilizing next generation sequencing this study evaluated the genetic diversity in the different facilities to determine whether the Salmonella serovars that were found are transient or resident. Salmonella serovars Montevideo, Ruiru, and Senftenberg were frequently detected, with Ruiru showing a particularly high predominance across multiple facilities, suggesting possible common sources of contamination including regional fishing waters and shared additives. The study also highlights the role of transportation and storage methods as a possible cause of cross-contamination. Future research should focus on identifying specific contamination sources and optimizing control measures to reduce Salmonella risks in fish meal production.
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publisher Public Library of Science (PLoS)
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spelling doaj-art-251fec190a6e4359b67a6f65783eb0fd2025-08-20T02:36:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032722210.1371/journal.pone.0327222Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.Johnathan LikensJon W BellPatricia RabideauStephanie HaynesSteven WilsonSandra TallentEric W BrownJulie HaendigesThis study evaluates Salmonella contamination in feed mill production facilities over a 12-year period, analyzing collection events from 12 facilities predominantly located in the southeastern United States. The genomic data reveals a historical contamination rate, with 20% of collection events testing positive for Salmonella. Utilizing next generation sequencing this study evaluated the genetic diversity in the different facilities to determine whether the Salmonella serovars that were found are transient or resident. Salmonella serovars Montevideo, Ruiru, and Senftenberg were frequently detected, with Ruiru showing a particularly high predominance across multiple facilities, suggesting possible common sources of contamination including regional fishing waters and shared additives. The study also highlights the role of transportation and storage methods as a possible cause of cross-contamination. Future research should focus on identifying specific contamination sources and optimizing control measures to reduce Salmonella risks in fish meal production.https://doi.org/10.1371/journal.pone.0327222
spellingShingle Johnathan Likens
Jon W Bell
Patricia Rabideau
Stephanie Haynes
Steven Wilson
Sandra Tallent
Eric W Brown
Julie Haendiges
Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
PLoS ONE
title Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
title_full Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
title_fullStr Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
title_full_unstemmed Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
title_short Transient and resident Salmonella: A genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities.
title_sort transient and resident salmonella a genomic approach to analyzing over a decade of sampling events from fish meal production and storage facilities
url https://doi.org/10.1371/journal.pone.0327222
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