The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco
Potassium (K+) plays a role in enzyme activation, membrane transport, and osmotic regulation processes. An increase in potassium content can significantly improve the elasticity and combustibility of tobacco and reduce the content of harmful substances. Here, we report that the expression analysis o...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2024.2359257 |
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| author | Li Xu Yifei Lu Jiarui Jiang Qian Chen Yong Xu Qili Mi Haiying Xiang Liming Lu Xuemei Li Qian Gao Liqin Li |
| author_facet | Li Xu Yifei Lu Jiarui Jiang Qian Chen Yong Xu Qili Mi Haiying Xiang Liming Lu Xuemei Li Qian Gao Liqin Li |
| author_sort | Li Xu |
| collection | DOAJ |
| description | Potassium (K+) plays a role in enzyme activation, membrane transport, and osmotic regulation processes. An increase in potassium content can significantly improve the elasticity and combustibility of tobacco and reduce the content of harmful substances. Here, we report that the expression analysis of Nt GF14e, a 14-3-3 gene, increased markedly after low-potassium treatment (LK). Then, chlorophyll content, POD activity and potassium content, were significantly increased in overexpression of Nt GF14e transgenic tobacco lines compared with those in the wild type plants. The net K+ efflux rates were severely lower in the transgenic plants than in the wild type under LK stress. Furthermore, transcriptome analysis identified 5708 upregulated genes and 2787 downregulated genes between Nt GF14e overexpressing transgenic tobacco plants. The expression levels of some potassium-related genes were increased, such as CBL-interacting protein kinase 2 (CIPK2), Nt CIPK23, Nt CIPK25, H+-ATPase isoform 2 a (AHA2a), Nt AHA4a, Stelar K+ outward rectifier 1(SKOR1), and high affinity K+ transporter 5 (HAK5). The result of yeast two-hybrid and luciferase complementation imaging experiments suggested Nt GF14e could interact with CIPK2. Overall, these findings indicate that NtGF14e plays a vital roles in improving tobacco LK tolerance and enhancing potassium nutrition signaling pathways in tobacco plants. |
| format | Article |
| id | doaj-art-251e7d89bddf47dbb5a78c7d8a5ae5a4 |
| institution | DOAJ |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-251e7d89bddf47dbb5a78c7d8a5ae5a42025-08-20T02:57:44ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242024-12-0119110.1080/15592324.2024.23592572359257The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobaccoLi Xu0Yifei Lu1Jiarui Jiang2Qian Chen3Yong Xu4Qili Mi5Haiying Xiang6Liming Lu7Xuemei Li8Qian Gao9Liqin Li10R&D Center of China Tobacco Yunnan Industrial Co. LtdSichuan Agriculture UniversityR&D Center of China Tobacco Yunnan Industrial Co. LtdSichuan Agriculture UniversityR&D Center of China Tobacco Yunnan Industrial Co. LtdR&D Center of China Tobacco Yunnan Industrial Co. LtdR&D Center of China Tobacco Yunnan Industrial Co. LtdSichuan Agriculture UniversityR&D Center of China Tobacco Yunnan Industrial Co. LtdR&D Center of China Tobacco Yunnan Industrial Co. LtdSichuan Agriculture UniversityPotassium (K+) plays a role in enzyme activation, membrane transport, and osmotic regulation processes. An increase in potassium content can significantly improve the elasticity and combustibility of tobacco and reduce the content of harmful substances. Here, we report that the expression analysis of Nt GF14e, a 14-3-3 gene, increased markedly after low-potassium treatment (LK). Then, chlorophyll content, POD activity and potassium content, were significantly increased in overexpression of Nt GF14e transgenic tobacco lines compared with those in the wild type plants. The net K+ efflux rates were severely lower in the transgenic plants than in the wild type under LK stress. Furthermore, transcriptome analysis identified 5708 upregulated genes and 2787 downregulated genes between Nt GF14e overexpressing transgenic tobacco plants. The expression levels of some potassium-related genes were increased, such as CBL-interacting protein kinase 2 (CIPK2), Nt CIPK23, Nt CIPK25, H+-ATPase isoform 2 a (AHA2a), Nt AHA4a, Stelar K+ outward rectifier 1(SKOR1), and high affinity K+ transporter 5 (HAK5). The result of yeast two-hybrid and luciferase complementation imaging experiments suggested Nt GF14e could interact with CIPK2. Overall, these findings indicate that NtGF14e plays a vital roles in improving tobacco LK tolerance and enhancing potassium nutrition signaling pathways in tobacco plants.http://dx.doi.org/10.1080/15592324.2024.2359257nt14-3-3potassiumtranscriptomecipktobacco |
| spellingShingle | Li Xu Yifei Lu Jiarui Jiang Qian Chen Yong Xu Qili Mi Haiying Xiang Liming Lu Xuemei Li Qian Gao Liqin Li The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco Plant Signaling & Behavior nt14-3-3 potassium transcriptome cipk tobacco |
| title | The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco |
| title_full | The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco |
| title_fullStr | The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco |
| title_full_unstemmed | The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco |
| title_short | The 14-3-3 protein nt GF14e interacts with CIPK2 and increases low potassium stress in tobacco |
| title_sort | 14 3 3 protein nt gf14e interacts with cipk2 and increases low potassium stress in tobacco |
| topic | nt14-3-3 potassium transcriptome cipk tobacco |
| url | http://dx.doi.org/10.1080/15592324.2024.2359257 |
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