An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment

Abstract Effective antibacterial therapy while accelerating the repair of bone defects is crucial for the treatment of osteomyelitis. Inspired by the protective mechanism of Andrias davidianus, we constructed an antibacterial hydrogel scaffold with excellent rigidity and long-term slow-release activ...

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Main Authors: Chong Yin, Meng Deng, Jinshu Yu, Yonghao Chen, Kaiyuan Zheng, Yi Huang, Xudong Deng, Ye Tian, Yuwen Ma, Beilei Zeng, Xiaolan Guo, Bin Guo
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-024-75997-8
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author Chong Yin
Meng Deng
Jinshu Yu
Yonghao Chen
Kaiyuan Zheng
Yi Huang
Xudong Deng
Ye Tian
Yuwen Ma
Beilei Zeng
Xiaolan Guo
Bin Guo
author_facet Chong Yin
Meng Deng
Jinshu Yu
Yonghao Chen
Kaiyuan Zheng
Yi Huang
Xudong Deng
Ye Tian
Yuwen Ma
Beilei Zeng
Xiaolan Guo
Bin Guo
author_sort Chong Yin
collection DOAJ
description Abstract Effective antibacterial therapy while accelerating the repair of bone defects is crucial for the treatment of osteomyelitis. Inspired by the protective mechanism of Andrias davidianus, we constructed an antibacterial hydrogel scaffold with excellent rigidity and long-term slow-release activity. While retaining the toughness of the skin secretion of Andrias davidianus (SSAD), the rigidity of the hydrogel material is increased by incorporating hydroxyapatite to meet the demands of bone-defect-filling materials. It also exerted antibacterial effects via the slow-release of vancomycin from local osteomyelitis lesions. Notably, the hydrogel can also carry a high stable recombinant miR-214-3p inhibitor (MSA-anti214). By the delivery of nano vector polyvinylamine, the long-term slow-release of MSA-anti214 is achieved to promote bone repair, making this composite hydrogel a potential SSAD-based osteomyelitis alleviator (SOA). In vitro and vivo results verified that the SOA effectively eliminated Staphylococcus aureus and repaired bone defects, ultimately mitigating the progression of osteomyelitis. This composite hydrogel extends the economic application prospects of A. davidianus and has provided new insights for the treatment of osteomyelitis. The study also explored new insights for the bone filling materials of bone defection and other skeletal system diseases.
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spelling doaj-art-251a6b5200e54fcf8e59de647b3ff6d02025-08-20T02:17:37ZengNature PortfolioScientific Reports2045-23222024-10-0114111710.1038/s41598-024-75997-8An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatmentChong Yin0Meng Deng1Jinshu Yu2Yonghao Chen3Kaiyuan Zheng4Yi Huang5Xudong Deng6Ye Tian7Yuwen Ma8Beilei Zeng9Xiaolan Guo10Bin Guo11Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Rehabilitation Medicine, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeKey Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical UniversityKey Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical UniversityDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Oncology, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical CollegeAbstract Effective antibacterial therapy while accelerating the repair of bone defects is crucial for the treatment of osteomyelitis. Inspired by the protective mechanism of Andrias davidianus, we constructed an antibacterial hydrogel scaffold with excellent rigidity and long-term slow-release activity. While retaining the toughness of the skin secretion of Andrias davidianus (SSAD), the rigidity of the hydrogel material is increased by incorporating hydroxyapatite to meet the demands of bone-defect-filling materials. It also exerted antibacterial effects via the slow-release of vancomycin from local osteomyelitis lesions. Notably, the hydrogel can also carry a high stable recombinant miR-214-3p inhibitor (MSA-anti214). By the delivery of nano vector polyvinylamine, the long-term slow-release of MSA-anti214 is achieved to promote bone repair, making this composite hydrogel a potential SSAD-based osteomyelitis alleviator (SOA). In vitro and vivo results verified that the SOA effectively eliminated Staphylococcus aureus and repaired bone defects, ultimately mitigating the progression of osteomyelitis. This composite hydrogel extends the economic application prospects of A. davidianus and has provided new insights for the treatment of osteomyelitis. The study also explored new insights for the bone filling materials of bone defection and other skeletal system diseases.https://doi.org/10.1038/s41598-024-75997-8Andrias davidianusOsteomyelitisNatural polymer-based hydrogelsNucleic acid drugsBone-defect-filling materials
spellingShingle Chong Yin
Meng Deng
Jinshu Yu
Yonghao Chen
Kaiyuan Zheng
Yi Huang
Xudong Deng
Ye Tian
Yuwen Ma
Beilei Zeng
Xiaolan Guo
Bin Guo
An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
Scientific Reports
Andrias davidianus
Osteomyelitis
Natural polymer-based hydrogels
Nucleic acid drugs
Bone-defect-filling materials
title An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
title_full An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
title_fullStr An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
title_full_unstemmed An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
title_short An Andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
title_sort andrias davidianus derived composite hydrogel with enhanced antibacterial and bone repair properties for osteomyelitis treatment
topic Andrias davidianus
Osteomyelitis
Natural polymer-based hydrogels
Nucleic acid drugs
Bone-defect-filling materials
url https://doi.org/10.1038/s41598-024-75997-8
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