Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang
Background: The present study aimed to investigate the anti-inflammatory and antioxidant effects of Sipyungtang using lipopolysaccharide (LPS)-treated RAW 264.7 cells. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromided (MTT) assay (Bio Basic)...
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| Format: | Article |
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MEDrang Inc.
2025-02-01
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| Series: | Journal of Acupuncture Research |
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| author | Na Young Jo Eun Yong Lee Jeong Du Roh Cham Kyul Lee |
| author_facet | Na Young Jo Eun Yong Lee Jeong Du Roh Cham Kyul Lee |
| author_sort | Na Young Jo |
| collection | DOAJ |
| description | Background: The present study aimed to investigate the anti-inflammatory and antioxidant effects of Sipyungtang using lipopolysaccharide (LPS)-treated RAW 264.7 cells. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromided (MTT) assay (Bio Basic). RAW 264.7 cells were cultured and treated with LPS to verify the inhibitory effects of Sipyungtang on nitric oxide (NO) production and cytokine generation and its antioxidant effect was assessed using 2,2-diphenyl-1-picrylhydrazyl. Results: Sipyungtang showed no cytotoxicity at concentrations of < 7.50 mg/mL. NO production was inhibited by Sipyungtang in a concentration-dependent manner, with the inhibitory effect observed at concentrations ranging from 0.47 mg/mL to 3.75 mg/mL. Regarding inhibiting cytokine production, interleukin (IL)-1β levels reduced markedly upon Sipyungtang treatment at concentrations of < 0.94 mg/mL, those of IL-6 at concentrations < 1.88 mg/mL, and tumor necrosis factor (TNF)-α at concentrations < 0.94 mg/mL. The antioxidant assay revealed that Sipyungtang exerted antioxidant effects ranging from 21.29% ± 1.72% to 72.20% ± 1.00% at concentrations ranging from 0.16 to 2.50 mg/mL. Conclusion: Our data indicate that Sipyungtang is safe, inhibits NO production, exhibits anti-inflammatory effects by reducing the production of cytokines IL-1β, IL-6, and TNF-α, and has antioxidant properties. Based on these findings, Sipyungtang may have potential applications in treating diseases caused by inflammation. |
| format | Article |
| id | doaj-art-25142e1cf6ea4a2bb89b0e272f237fbe |
| institution | OA Journals |
| issn | 2586-288X 2586-2898 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MEDrang Inc. |
| record_format | Article |
| series | Journal of Acupuncture Research |
| spelling | doaj-art-25142e1cf6ea4a2bb89b0e272f237fbe2025-08-20T02:02:06ZengMEDrang Inc.Journal of Acupuncture Research2586-288X2586-28982025-02-0142110311010.13045/jar.24.0068Study on the Anti-inflammatory and Antioxidant Effects of SipyungtangNa Young Jo0https://orcid.org/0000-0003-2802-2626Eun Yong Lee1https://orcid.org/0000-0001-5819-1602Jeong Du Roh2https://orcid.org/0000-0002-5512-6765Cham Kyul Lee3https://orcid.org/0000-0002-0156-310XDepartment of Acupuncture and Moxibustion Medicine, Jecheon Hospital of Korean Medicine, Semyung University, Jecheon, KoreaDepartment of Acupuncture and Moxibustion Medicine, Chungju Hospital of Korean Medicine, Semyung University, Chungju, Korea Department of Acupuncture and Moxibustion Medicine, Jecheon Hospital of Korean Medicine, Semyung University, Jecheon, Korea Department of Acupuncture and Moxibustion Medicine, Chungju Hospital of Korean Medicine, Semyung University, Chungju, KoreaBackground: The present study aimed to investigate the anti-inflammatory and antioxidant effects of Sipyungtang using lipopolysaccharide (LPS)-treated RAW 264.7 cells. Methods: Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromided (MTT) assay (Bio Basic). RAW 264.7 cells were cultured and treated with LPS to verify the inhibitory effects of Sipyungtang on nitric oxide (NO) production and cytokine generation and its antioxidant effect was assessed using 2,2-diphenyl-1-picrylhydrazyl. Results: Sipyungtang showed no cytotoxicity at concentrations of < 7.50 mg/mL. NO production was inhibited by Sipyungtang in a concentration-dependent manner, with the inhibitory effect observed at concentrations ranging from 0.47 mg/mL to 3.75 mg/mL. Regarding inhibiting cytokine production, interleukin (IL)-1β levels reduced markedly upon Sipyungtang treatment at concentrations of < 0.94 mg/mL, those of IL-6 at concentrations < 1.88 mg/mL, and tumor necrosis factor (TNF)-α at concentrations < 0.94 mg/mL. The antioxidant assay revealed that Sipyungtang exerted antioxidant effects ranging from 21.29% ± 1.72% to 72.20% ± 1.00% at concentrations ranging from 0.16 to 2.50 mg/mL. Conclusion: Our data indicate that Sipyungtang is safe, inhibits NO production, exhibits anti-inflammatory effects by reducing the production of cytokines IL-1β, IL-6, and TNF-α, and has antioxidant properties. Based on these findings, Sipyungtang may have potential applications in treating diseases caused by inflammation.anti-inflammatory agentsantioxidantscytokineskorean medicinesipyungtang |
| spellingShingle | Na Young Jo Eun Yong Lee Jeong Du Roh Cham Kyul Lee Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang Journal of Acupuncture Research anti-inflammatory agents antioxidants cytokines korean medicine sipyungtang |
| title | Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang |
| title_full | Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang |
| title_fullStr | Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang |
| title_full_unstemmed | Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang |
| title_short | Study on the Anti-inflammatory and Antioxidant Effects of Sipyungtang |
| title_sort | study on the anti inflammatory and antioxidant effects of sipyungtang |
| topic | anti-inflammatory agents antioxidants cytokines korean medicine sipyungtang |
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