T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device
Introduction: The challenges related to the diagnosis of sexually transmitted infections present more complex factors in remote and hard-to-reach areas. The use of self-collection devices that facilitate the obtaining of a biological sample with high quality for sensitive molecular tests have been...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2019-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11385 |
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| author | Danielle AP Rocha Maria Joana N Azevedo Sávio José S Batista Êmille S Beltrão Cássia O Moraes Adriene F Araújo Renato S Reis Kátia L Torres José E Levi Josiane M Mariño |
| author_facet | Danielle AP Rocha Maria Joana N Azevedo Sávio José S Batista Êmille S Beltrão Cássia O Moraes Adriene F Araújo Renato S Reis Kátia L Torres José E Levi Josiane M Mariño |
| author_sort | Danielle AP Rocha |
| collection | DOAJ |
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Introduction: The challenges related to the diagnosis of sexually transmitted infections present more complex factors in remote and hard-to-reach areas. The use of self-collection devices that facilitate the obtaining of a biological sample with high quality for sensitive molecular tests have been examined. This study aimed to evaluate the performance and acceptance of the Evalyn® Brush (Rovers® Medical Devices) for detection of T. vaginalis among women living in the riverside communities of Amazonas, Brazil.
Methodology: The study included 300 riverside women. They received instructions for self-collection, carried out the task, and then answered a questionnaire on the use of the device. T. vaginalis was detected by Polymerase Chain Reaction, using primers TVK3/TVK7.
Results: The mean age of the women was 35.8 years, and most of them presented low schooling, low income, agricultural activity and lived in a marital union. All samples were positive for human genomic DNA (100%) and the prevalence of T. vaginalis infection was 5.6% (n = 17). Of the 300 women, 293 (97.7%) indicated that they liked the use of the device, 287 (95.7%) reported having had no difficulty in handling it, 265 (88.3%) did not feel any type of discomfort and 228 (76%) said they preferred the self-collection to the collection made by the professional, mainly due to privacy and comfort.
Conclusions: The Evalyn® Brush proved reliable as a device for the collection of biological samples for molecular analysis and was well-accepted by women. Its use can be indicated in remote and hard to reach places.
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| format | Article |
| id | doaj-art-250706f513614e2d8a16dd1241393222 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-250706f513614e2d8a16dd12413932222025-08-20T02:14:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-11-01131110.3855/jidc.11385T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection deviceDanielle AP Rocha0Maria Joana N Azevedo1Sávio José S Batista2Êmille S Beltrão3Cássia O Moraes4Adriene F Araújo5Renato S Reis6Kátia L Torres7José E Levi8Josiane M Mariño9Instituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, BrasilFundação Centro de Controle em Oncologia do Estado do Amazonas (FCECON), Manaus, Amazonas, BrasilInstituto de Medicina Tropical (IMT), Universidade de São Paulo (USP), São Paulo, São Paulo, BrasilInstituto de Saúde e Biotecnologia (ISB), Universidade Federal do Amazonas (UFAM), Coari, Amazonas, Brasil Introduction: The challenges related to the diagnosis of sexually transmitted infections present more complex factors in remote and hard-to-reach areas. The use of self-collection devices that facilitate the obtaining of a biological sample with high quality for sensitive molecular tests have been examined. This study aimed to evaluate the performance and acceptance of the Evalyn® Brush (Rovers® Medical Devices) for detection of T. vaginalis among women living in the riverside communities of Amazonas, Brazil. Methodology: The study included 300 riverside women. They received instructions for self-collection, carried out the task, and then answered a questionnaire on the use of the device. T. vaginalis was detected by Polymerase Chain Reaction, using primers TVK3/TVK7. Results: The mean age of the women was 35.8 years, and most of them presented low schooling, low income, agricultural activity and lived in a marital union. All samples were positive for human genomic DNA (100%) and the prevalence of T. vaginalis infection was 5.6% (n = 17). Of the 300 women, 293 (97.7%) indicated that they liked the use of the device, 287 (95.7%) reported having had no difficulty in handling it, 265 (88.3%) did not feel any type of discomfort and 228 (76%) said they preferred the self-collection to the collection made by the professional, mainly due to privacy and comfort. Conclusions: The Evalyn® Brush proved reliable as a device for the collection of biological samples for molecular analysis and was well-accepted by women. Its use can be indicated in remote and hard to reach places. https://jidc.org/index.php/journal/article/view/11385Self-collectionTrichomonas vaginalisAmazonas |
| spellingShingle | Danielle AP Rocha Maria Joana N Azevedo Sávio José S Batista Êmille S Beltrão Cássia O Moraes Adriene F Araújo Renato S Reis Kátia L Torres José E Levi Josiane M Mariño T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device Journal of Infection in Developing Countries Self-collection Trichomonas vaginalis Amazonas |
| title | T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device |
| title_full | T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device |
| title_fullStr | T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device |
| title_full_unstemmed | T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device |
| title_short | T. vaginalis in riverside women in Amazonia, Brazil: an experience using the EVALYN® BRUSH vaginal self-collection device |
| title_sort | t vaginalis in riverside women in amazonia brazil an experience using the evalyn r brush vaginal self collection device |
| topic | Self-collection Trichomonas vaginalis Amazonas |
| url | https://jidc.org/index.php/journal/article/view/11385 |
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